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Sérgio Lúcio Pereira de Castro Lopes,Isadora Luana Flores,Thiago de Oliveira Gamba,Rivea Ines Ferreira-Santos,Mari Eli Leonelli de Moraes,Aline Alvarez Cabello,Paula Nascimento Moutinho 대한구강악안면외과학회 2017 대한구강악안면외과학회지 Vol.43 No.2
Maxillofacial images must be examined to find pathologies not identified during clinical examination. Unicystic ameloblastoma (UA) extending to the mandibular body and ramus was neglected on initial panoramic radiographic examination. After orthodontic therapy, a huge lesion was observed clinically and through imaging exams. After the conservative surgery, no recurrence was observed during five years of follow-up. This case emphasized the need for careful evaluation of patient images focusing on the oral diagnosis before any dental treatment planning, including orthodontic therapy.
Lateral pterygoid muscle volume and migraine in patients with temporomandibular disorders
Sérgio Lúcio Pereira de Castro Lopes,André Luiz Ferreira Costa,Thiago de Oliveira Gamba,Isadora Luana Flores,Adriana Dibo Cruz,Li Li Min 대한영상치의학회 2015 Imaging Science in Dentistry Vol.45 No.1
Purpose: Lateral pterygoid muscle (LPM) plays an important role in jaw movement and has been implicated in Temporomandibular disorders (TMDs). Migraine has been described as a common symptom in patients with TMDs and may be related to muscle hyperactivity. This study aimed to compare LPM volume in individuals with and without migraine, using segmentation of the LPM in magnetic resonance (MR) imaging of the TMJ. Materials and Methods: Twenty patients with migraine and 20 volunteers without migraine underwent a clinical examination of the TMJ, according to the Research Diagnostic Criteria for TMDs. MR imaging was performed and the LPM was segmented using the ITK-SNAP 1.4.1 software, which calculates the volume of each segmented structure in voxels per cubic millimeter. The chi-squared test and the Fisher’s exact test were used to relate the TMD variables obtained from the MR images and clinical examinations to the presence of migraine. Logistic binary regression was used to determine the importance of each factor for predicting the presence of a migraine headache. Results: Patients with TMDs and migraine tended to have hypertrophy of the LPM (58.7%). In addition, abnormal mandibular movements (61.2%) and disc displacement (70.0%) were found to be the most common signs in patients with TMDs and migraine. Conclusion: In patients with TMDs and simultaneous migraine, the LPM tends to be hypertrophic. LPM segmentation on MR imaging may be an alternative method to study this muscle in such patients because the hypertrophic LPM is not always palpable.
Lentinula edodes (Shiitake) Modulates Chemically Induced Mutagenesis by Enhancing Pitting
Patrı´cia L. Alves de Lima,Marina M. Sugui,Angela I.M. Petrı´cio,Lı´zia C. Vilela,Andre´a V.F. Pinto,Priscila R. Martins,Ramon Kaneno,Daniel A. Ribeiro,Daisy M.F. Salvadori,Lu´cia R. Ribeiro 한국식품영양과학회 2013 Journal of medicinal food Vol.16 No.8
This study was undertaken to understand how Lentinula edodes modulates in vivo mutagenesis induced by alkylating agents in bone marrow and peripheral blood as described in our previous article. Male Swiss mice were pretreated for 15 consecutive days with aqueous extracts prepared from L. edodes, after which, the number of circulating blood cells, normal erythroid bone marrow cell cycling, and phagocytosis of micronucleated reticulocyte (MNRET) and activation of spleen macrophages were assessed. The results indicate that the antimutagenicity seen in bone marrow and peripheral blood is exerted by distinct compounds with different actions. The antimutagenic effect in bone marrow is exerted by compounds subject to degradation at deep-freeze storage temperature of - 20 C. On the other hand, compounds responsible for antimutagenicity in peripheral blood are not subject to degradation at - 20 C. The results also indicate that the antimutagenic action in peripheral blood leading to the reduction of circulating MNRET occurs in the spleen primarily through a phagocytic activity due to higher macrophage numbers and probably not due to the enhanced activation state of individual cells.
Regina Mambeli Barros,Geraldo Lúcio Tiago Filho,Ivan Felipe Silva dos Santos,Fernando das Graças Braga da Silva 한국유체기계학회 2015 International journal of fluid machinery and syste Vol.8 No.1
This study aims to present a hydraulic transitory study as MOC applications for solving the Saint-Venant equations in two case studies: 1) in a penstock of a small hydropower system as a simple pipeline in the case of valve-closure in the downstream boundary with a reservoir in the upstream boundary; and 2) for discharge propagation into a channel by velocity and depth of the flow channel along space evaluation. The proposed methodology by Chaudry [5] concerning the development of hydrodynamic models was used. The obtained results for first and second case study has been confirmed that MOC numerical approach is useful for several engineering purposes, including cases of hydraulic transients and discharge propagation in hydraulic systems.
Xanthomonas axonopodis pv. eucalyptorum pv. nov. Causing Bacterial Leaf Blight on Eucalypt in Brazil
Hélvio Gledson Maciel Ferraz,Jorge Luis Badel,Lúcio Mauro da Silva Guimarães,Bruna Paolinelli Reis,Marcos Rogério Tótola,Rivadalve Coelho Gonçalves,Acelino Couto Alfenas 한국식물병리학회 2018 Plant Pathology Journal Vol.34 No.4
Bacterial leaf blight is a major disease of eucalypt, especially under nursery conditions. Different bacterial species have been associated with the disease in several countries, and despite its importance worldwide, it is not clear to date whether similar disease symptoms are caused by the same or by different etiological agents. In this study, 43 bacterial strains were isolated from blighted eucalypt leaves collected in different geographic areas of Brazil and inoculated onto a susceptible eucalypt clone. Polyphasic taxonomy, including morphological, physiological, biochemical, molecular, and pathogenicity tests showed that only certain strains of Xanthomonas axonopodis caused symptoms of the disease. Strains varied in their aggressiveness, but no correlation with geographic origin was observed. MLSA-based phylogenetic analysis using concatenated dnaK, fyuA, gyrB and rpoD gene sequences allocated the strains in a well-defined clade, corresponding to Rademarker’s group RG 9.6. Inoculation of nineteen plant species belonging to seven botanical families with representative strain LPF 602 showed it to be pathogenic only on Eucalyptus spp, and Corymbia spp. Based on distinct biochemical and pathogenic characteristics that differentiate the eucalypt strains from other pathovars of the X. axonopodis species, here we propose their allocation into the new pathovar X. axonopodis pv. eucalyptorum pv. nov.
Abdala-Junior Reinaldo,No-Cortes Juliana,Arita Emiko Saito,Ackerman Jerome L.,da Silva Renan Lúcio Berbel,Kim Jun Ho,Cortes Arthur Rodriguez Gonzalez 대한영상치의학회 2021 Imaging Science in Dentistry Vol.51 No.4
Purpose: The aim of this in vitro study was to assess the role of bandwidth on the area of magnetic resonance imaging (MRI) artifacts caused by orthodontic appliances composed of different alloys, using different pulse sequences in 1.5 T and 3.0 T magnetic fields. Materials and Methods: Different phantoms containing orthodontic brackets (ceramic, ceramic bracket with a stainless-steel slot, and stainless steel) were immersed in agar gel and imaged in 1.5 T and 3.0 T MRI scanners. Pairs of gradient-echo (GE), spin-echo (SE), and ultrashort echo time (UTE) pulse sequences were used differing in bandwidth only. The area of artifacts from orthodontic devices was automatically estimated from pixel value thresholds within a region of interest (ROI). Mean values for similar pulse sequences differing in bandwidth were compared at 1.5 T and 3.0 T using analysis of variance. Results: The comparison of groups revealed a significant inverse association between bandwidth values and artifact areas of the stainless-steel bracket and the self-ligating ceramic bracket with a stainless-steel slot (P<0.05). The areas of artifacts from the ceramic bracket were the smallest, but were not reduced significantly in pulse sequences with higher bandwidth values (P<0.05). Significant differences were also observed between 1.5 T and 3.0 T MRI using SE and UTE, but not using GE 2-dimensional or 3-dimensional pulse sequences. Conclusion: Higher receiver bandwidth might be indicated to prevent artifacts from orthodontic appliances in 1.5 T and 3.0 T MRI using SE and UTE pulse sequences.
Queiroz Polyane Mazucatto,Fardim Karolina Castilho,Costa André Luiz Ferreira,Matheus Ricardo Alves,Lopes Sérgio Lúcio Pereira Castro 대한영상치의학회 2023 Imaging Science in Dentistry Vol.53 No.2
Purpose: The aim of this study was to evaluate changes in the trabecular bone through texture analysis and compare the texture analysis characteristics of different areas in patients with medication-related osteonecrosis of the jaw (MRONJ). Materials and Methods: Cone-beam computed tomographic images of 16 patients diagnosed with MRONJ were used. In sagittal images, 3 regions were chosen: active osteonecrosis (AO); intermediate tissue (IT), which presented a zone of apparently healthy tissue adjacent to the AO area; and healthy bone tissue (HT) (control area). Texture analysis was performed evaluating 7 parameters: secondary angular momentum, contrast, correlation, sum of squares, inverse moment of difference, sum of entropies, and entropy. Data were analyzed using the Kruskal-Wallis test with a significance level of 5%. Results: Comparing the areas of AO, IT, and HT, significant differences (P<0.05) were observed. The IT and AO area images showed higher values for parameters such as contrast, entropy, and secondary angular momentum than the HT area, indicating greater disorder in these tissues. Conclusion: Through texture analysis, changes in the bone pattern could be observed in areas of osteonecrosis. The texture analysis demonstrated that areas visually identified and classified as IT still had necrotic tissue, thereby increasing the accuracy of delimiting the real extension of MRONJ.
Costa Andre Luiz Ferreira,Fardim Karolina Aparecida Castilho,Ribeiro Isabela Teixeira,Jardini Maria Aparecida Neves,Braz-Silva Paulo Henrique,Orhan Kaan,de Castro Lopes Sérgio Lúcio Pereira 대한영상치의학회 2023 Imaging Science in Dentistry Vol.53 No.1
Purpose: This study aimed to assess texture analysis (TA) of cone-beam computed tomography (CBCT) images as a quantitative tool for the differential diagnosis of odontogenic and non-odontogenic maxillary sinusitis (OS and NOS, respectively). Materials and Methods: CBCT images of 40 patients diagnosed with OS (N = 20) and NOS (N = 20) were evaluated. The gray level co-occurrence (GLCM) matrix parameters, and gray level run length matrix texture (GLRLM) parameters were extracted using manually placed regions of interest on lesion images. Seven texture parameters were calculated using GLCM and 4 parameters using GLRLM. The Mann-Whitney test was used for comparisons between the groups, and the Levene test was performed to confirm the homogeneity of variance (α = 5%). Results: The results showed statistically significant differences (P<0.05) between the OS and NOS patients regarding 3 TA parameters. NOS patients presented higher values for contrast, while OS patients presented higher values for correlation and inverse difference moment. Greater textural homogeneity was observed in the OS patients than in the NOS patients, with statistically significant differences in standard deviations between the groups for correlation, sum of squares, sum of entropy, and entropy. Conclusion: TA enabled quantitative differentiation between OS and NOS on CBCT images by using the parameters of contrast, correlation, and inverse difference moment.
Cláudia Santos Gai,Paulo Teixeira Lacava,Maria Carolina Quecine,Marie-Christine Auriac,João Roberto Spotti Lopes,Welington Luiz Araújo,Thomas Albert Miller,João Lúcio Azevedo 한국미생물학회 2009 The journal of microbiology Vol.47 No.4
Methylobacterium mesophilicum, originally isolated as an endophytic bacterium from citrus plants, was genetically transformed to express green fluorescent protein (GFP). The GFP-labeled strain of M. mesophilicum was inoculated into Catharanthus roseus (model plant) seedlings and further observed colonizing its xylem vessels. The transmission of this endophyte by Bucephalogonia xanthophis, one of the insect vectors that transmit Xylella fastidiosa subsp. pauca, was verified by insects feeding from fluids containing the GFP bacterium followed by transmission to plants and isolating the endophyte from C. roseus plants. Forty-five days after inoculation, the plants exhibited endophytic colonization by M. mesophilicum, confirming this bacterium as a nonpathogenic, xylem-associated endophyte. Our data demonstrate that M. mesophilicum not only occupy the same niche of X. fastidiosa subsp. pauca inside plants but also may be transmitted by B. xanthophis. The transmission, colonization, and genetic manipulation of M. mesophilicum is a prerequisite to examining the potential use of symbiotic control to interrupt the transmission of X. fastidiosa subsp. pauca, the bacterial pathogen causing Citrus variegated chlorosis by insect vectors.
Wagner L. Gonçalves,Guilherme F. Gomes,Yohan D. Mendéz,Fabrício A. Almeida,Valquíria C. Santos,Sebastião S. Cunha Jr. 사단법인 한국계산역학회 2018 Computers and Concrete, An International Journal Vol.22 No.1
Reinforced concrete structures are widely used in civil engineering projects around the world in different designs. Due to the great evolution in computational equipment and numerical methods, structural analysis has become more and more reliable, and in turn more closely approximates reality. Thus among the many numerical methods used to carry out these types of analyses, the finite element method has been highlighted as an optimized tool option, combined with the non-linear and linear analysis techniques of structures. In this paper, the behavior of reinforced concrete beams was analyzed in two different configurations: i) with welding and ii) conventionally lashed stirrups using annealed wire. The structures were subjected to normal and tangential forces up to the limit of their bending resistance capacities to observe the cracking process and growth of the concrete structure. This study was undertaken to evaluate the effectiveness of welded wire fabric as shear reinforcement in concrete prismatic beams under static loading conditions. Experimental analysis was carried out in order compare the maximum load of both configurations, the experimental load-time profile applied in the first configuration was used to reproduce the same loading conditions in the numerical simulations. Thus, comparisons between the numerical and experimental results of the welded frame beam show that the proposed model can estimate the concrete strength and failure behavior accurately.