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RISS 인기검색어
- 검색키워드
- 저자 : Kyung Ho Kang(姜京鎬)Soo Yeon Cho(趙守衍) (검색결과 2 건)
- 무료
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Huhn Pal Moon(文憲八),Kyung Ho Kang(姜京鎬)Soo Yeon Cho(趙守衍) 한국육종학회 1991 한국육종학회지 Vol.23 No.2
One-step culture in which the pollen plantlets are produced directly from either embryoids or embryogenic callus from microspores in a single operation of the culture procedure would save labors, time, laboratory materials and spaces required with two-step culture in which callus are induced in a medium and subsequently transferred to the different medium for plant development. Three phytohormones, 2,4-D, kinetin and ABA were added with combined formulations in N6 basal medium to determine the effects on the development of pollen plant through one-step procedure of anther culture without transferring callus. Two japonica(Cheonmabyeo and Chucheongbyeo) and two Tongil-type(indica/japonica) varieties(Milyang 23 and Taebaegbyeo) were used as donor plant materials. All the varieties used produced pollen plants in any one formulation of 24 treatment combinations from 4 weeks after anther plating with the large between- and within-varietal variability:0.6~26.3% for Cheonmabyeo, 0.3~13.5 % for Chucheongbyeo, 0~2.3 % for Milyang 23 and 01.5% for Taebaegbyeo. In general, the N₆ basic medium supplemented with 0.002-0.02mg/ℓ of 2,4-D showed higher plant regenerations for japonica varieties in one-step culture. The combination of 0.002mg/ℓ 2,4-D and 0.5mg/ℓ kinetin enhanced the frequency of direct plant production, while the effect of abscisic acid was not clear.
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벼 葯培養에서 低溫 前處理가 callus 및 植物體 分化率에 미치는 影響
Huhn Pal Moon(文憲八),Sun Hee Choi(崔善嬉),Kyung Ho Kang(姜京鎬),Soo Yeon Cho(趙守衍) 한국육종학회 1989 한국육종학회지 Vol.21 No.3
The increasing callus induction and plant regeneration is the first target to apply anther culture technique to rice varietal improvement. The effects of low-temperature pretreatment on the callus induction and plant regeneration were examined with different temperatures and different treatment durations in four rice varieties. Low-temperature treatment on rice anther prior to anther plating increased remarkably both callus induction and plant regeneration, especially in the callus induction. The treatment temperature was more critical than the duration. In general, the proper treatment would be 8-12℃ in temperature and 8-16 days in duration with higher temperature for Tongil type rice varieties, which were derived from indical/japonica hybridization. Highly significant correlation was found between the callus induction and plant regeneration in Tongil type rice varieties, Milyang 23 and Taebaegbyeo, while no significant correlation in Japonica varieties. The ratio of haploid and diploid plants among the anther-derived plants with lowtemperature pertreatment was varied from different rice varieties, averaging approximately 7 haploids : 3 diploids.
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