청반손상 후에 L-dopa 를 투여한 쥐에서 나타나는 주의장애

조옥경,한정수,김기석 한국심리학회 1989 한국심리학회지 생물 및 생리 Vol.1 No.1

도파민 효능제인 L-dopa를 쥐에게 투여하면 강화와 짝지어지지 않은 부적절자극을 무시하는 주의 과정에 장애가 나타난다. 이같은 장애는 청반을 파괴하여 대뇌 노어에피네프린계의 이상을 초래한 경우에도 나타나므로 청반손상 후에 L-dopa를 투여하면 주의장애가 심해질 것으로 예상하였다. 왕복상자에서 회피반응을 쥐에게 학습시킨 후 검사시행에서는 훈련에 사용되었던 소리자극을 전기쇼크없이 제시하는 중간중간에 부적절자극인 빛자극을 무선적으로 제시하였다. 실험 결과, L-dopa집단이 주의장애를 보였고 청반손상집단은 주의장애가 없었으나 청반손상과 L-dopa 투여가 같이 처치된 집단은 부적절자극을 무시하는 능력에 상당한 장애를 보였는데 이는 L-dopa 단독 투여 효과를 훨씬 넘어서는 것이었다. 이같은 결과는 주의과정에 도파민과 노어에피네프린이 상호작용할 가능성을 시사하며 이는 정신분열증 환자에서 나타나는 주의장애에 대한 동물모형을 제시해 줄 수 있다고 생각된다. The attentional process by which animals learn to ignore an irrelevant stimulus was examined after the treatment with L-dopa or bilateral electrolytic lesions of locus coeruleus(LC). Rats received daily 100 trials of two-way avoidance training in which each trial was initiated by a 10-sec tone stimulus and terminated either by a shuttle response during the tone(avoidance) or by a response during the electric shock(escape). The training sessions continued until the animals met a criterion of eight avoidance responses during any ten consecutive trials. The same rats then received a 50 trials test session. A light, which was irrelevant because it had never been paired with shock, was randomly presented during the test period. The administration of L-dopa produced marked impairment in tuning out the irrelevant stimulus, but LC lesion group did not show any attentional deficits. When L-dopa injection was superimposed to LC lesions, the deficit was increased. It may be interpreted that the result is due to an interaction between the noradrenergic and dopaminergic system. This interaction in ignoring an irrelevant stimulus may provide an animal model of selective attention deficit observed among some schizophrenic patients.

運動負荷前ㆍ後 血中 GLUCOSE, 蛋白質, 脂肪의 含量變化에 관한 硏究

文正玉,金斗卿 仁荷大學校 스포츠科學硏究所 1991 論文集 Vol.3 No.-

This study is to analyze and to measure blood by blood - gathering over the second investigation before and after 90 minutes (the first and latter half each 45 min and the middle rest 10 min.) soccer game the subject of 22 soccer players in business team in order to know the content change of Glucose, Fat and Protein in blood after game. The result is all follows : 1. The Glucose in blood was increased by 5.5mg/dl(7.0%), 78.82mg/dl efore game and 84.32mg/dl after game but it didn't regard in level of 95%. 2. The content of Fat in blood was increased by 11.45mg/dl(11.9% ), 96.6mg/dl before game and 108.09mg/dl after game but it didn't regard in level of 95%. 3. The content of protein in blood shows to increase 0.44mg/dl(6.4%),7.20mg/dl before game and 7.66mg/dl after game (p<0.05)

한국인에게 적절한 기관삽관 위치

이승한,최옥경,정구영,정성필 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

Endotracheal intubation is one of the important procedures in ED and various fields. Endobronchial intubation can lead to hypoxemia secondary to tension pneumothorax of intubated side and atelectasis of contralateral side. The placement of tube beyond the vocal cords may results in inadvertent extubation, larygeal spasm and aspiration pneumonia. The conformation methods of endotracheal intubation are auscultation of the chest, observation of a vapor trail, oximetry and capnography, and radiologic evaluation. Owen and associates recommended that oral intubation tubes at the upper incisor teeth at the 23cm in men and the 21cm in women of average adult size would have led to proper placement. We studied that whether the proper depth of endotracheal tube in American is appropriate to Korean. We studied adult patients admitted to ED (of both hospitals during March in 1995. Endotracheal tubes in men were positioned at the 23cm and women had positioned at the 21cm at the upper incisor teeth. The distance of the tip of the endotracheal tube to the tracheal carina on the postintubation chest X-ray then determined. Correct placement of intubation was defined with the tip of the tube≥3cm above the carina and below the level of the larynx. Fifty adult patients were enrolled in the study ; 29 men (58%) and 31 women (42%). Mean height and weight were 170±5.2cm, 68±9kg in men and 158±6.5cm, 56±10kg in women. Reasons for intubation were ; respiratory failure(38%), trauma(16%), increased intracramial pressare(16%), drug intoxication(12%), shock(8%), cardiac arrest(7%)and others(3%). The mean distance from the tip to carina on chest X-ray was 4.13±1.5cm in men and 3.45±2.1cm in women. Proper position was 27/29(93%) in men, 14/21(67%) in women or 41/50(82%). this means the placement of endotracheal tube of our patients was positioned deeper than prion study group(97.6%).(p<.05) We recommend that the 22cm tube mark for men and 20cm tube mard for women at upper incisor teeth would have led to proper placement in Korean adult patient.

병원전 응급처치의 시행정도와 정확도

이승한,최옥경,정구영 大韓應急醫學會 1996 대한응급의학회지 Vol.7 No.1

The Emergency Medical Technitian(EMT) is the main personnel of Emergency Medical Service Systems, requiring a whole set of special rescue skills and the knowledges for emergent patients in prehospital area. The most important aspects of the EMT system is the medical direction, which is education, protocol, and quality assurance especially in implementing phase. The EMT system just has been started in Korea with legislative effort of Emergency Medical Act since 1994. There has been no data about the frequencies of prehospital emergency care and it`s medical evaluation. Therefore, we investigated the volume of the prehospital emergency care performed by 119 rescue personnel, and assessed the accuracy of cardiopulmonary resuscitation(CPR) skills. We also analyzed which personal factors influence the accuracy of CPR skills by using the multiple regression test in SAS program. One hundred sixty four(64.5%) of the 259 rescuer graduated highschool, and 66.4% had less than 4 years career as a rescuer. Sixty one percentage has less than four times of CPR training. The interval from the last CPR training was less than 6 months in 59.1%, and less then 1 year in 71.4%. While over 75% of 119 rescuers had the experiences of primary emergency cares, about 50% had of CPR and of vital sign check. The accuracy of chest compression were 48.3%, 50.2%, and 52.2% in the rate, depth, and the site, but the accuracy of ventilation, and the ratio of inspiration and expiration. The accuracy of CPR was influenced only by times of CPR training(p=0.0083), not by the education level, the professional career, nor interval after the last training. From the above results, we could have conclusion that EMT training program should be focused to the initial patient assessment, and CPR training should be shared more time in ventilation than in chest compression. Also we could reach to a conclusion that it is important to increase the times of CPR training for improving the accuracy of CPR and that the proper interval of the training might be less than 3 months in early phase.

응급실에서 succinylcholine을 이용한 기관내 삽관시 혈청 칼륨의 변화

김무수,최옥경,어은경,정구영 大韓應急醫學會 1997 대한응급의학회지 Vol.8 No.1

Succinylcholine is a depolarizing neuromuscular blocking agent(NMB) and is used in induction of anesthesia. Succinylcholine-assisted endotracheal intubation has been routinely used in the operating room and rapid sequence intubation with NMB agent has been the method for airway management in emergency department(ED). Adverse effects of succinylcholine are hyperkalemia, malignant hyperthermia, transient hypotension, arrhythmia, and increased intracranial, intraocular and gastric pressure, etc. This study carried out the change of serum potassium as an adverse effects of succinylcholine during endotracheal intubation in ED. A prospective, noncontrolled design was used to study serial serum potassium levels in pre-and postendotracheal intubation(5min) from February 1996 to August 1996. Succinycholine was selectively used in patient with a ploblem of difficult endotracheal intubation (alert to drowsy mental status with intact gag reflex). The dose of succinylcholine was 1.5 mg/kg and injected into intravenous line during preoxygenation with bag-valve-mask device. Student's two-tailed t-test was used and p-value was<0.05. Among the total of 48 patients(male 27, female 21), the postintubation level of the serum potassium of 25 patients were increased and those of 21 persons were decreased compared with preintubation level. Two patient showed no change in amount. The average serum potassium indicated 3.7±0.8 mEq/L before the injection of the succinylcholine and 3.8±0.9 mEq/L 5 minutes after its injection. P-value showed more than 0.05 and as the result statistically there was no noteworthy difference. All patients were intubated within 30 sec and no patients were failed. We concluded that succinylcholine-assisted endotracheal intubation is a rapid and safe method as an endotracheal intubation practiced in ED and succinylcholin-induced hyperkalemia is miniimal and no adverse effect.

도시지역 응급센터를 내원한 약물중독 환자

유지영,최옥경,김무수,정구영 대한응급의학회 1995 대한응급의학회지 Vol.6 No.2

Acute drug intoxication is a important part of emergency medicine. The outcome of intoxicated patients depends upon a kind of drug, a dose of drug, the time of initial management and the knowledge of variable drugs. Our study was made on 249cases of acute drug intoxication, who visited to emergency department of Ehwa Wonams University Mokdong Hospital during 15months from June 1994 to August 1995. The patients of early 10 months period were treated in department in IM and patients of late 5 months period treated in department of EM. The results were as follows : 1) Total nember of patients was 249 which is 0.59% fo total ED patients. The male to female ratio was 1:2 (81pts : 168 pts) The age distribution showed the highest incidences in 2nd (35%) and 3rd (23%) decades. 2) The most patients (78%) were visited to ED within 4 hour after drug ingestion. 3) The most common drug which activated charcoal was indicated was sedatives and hynotics (63%) The most common drug which activated charcoal was contraindicated was strong acid-alkali (4%) 4) The total mortality of intoxicated patients was 8%. 5) The outcomes of the patients( early 10 months vs. late 5 months) : the percentage of admission was 20% vs 24%, discharge against admission was 36% vs 46% discharge was 21% vs 22%, transfer to other hospital was 22% vs 9%

요로결석 환자에 대한 응급실에서의 진단방법

유지영,최옥경,유진현,정구영 大韓應急醫學會 1997 대한응급의학회지 Vol.8 No.1

Nephrolithiasis is one of the most common disease seen in the Emergency Department. Among the patients who visited in the Emergency Department with acute abdominal pain, many people were suspected urolithiasis. Because of limitation of diagnostic method on Emergency Department we planned this study in order to confirm the our accuracy of diagnosis and the possibility of misdiagnosis. During 5 months from April 1996 to August 1996, the 189 patients was suspected the urolithiasis. Among this patient, 51 cases was excluded due to incomplete study. After performing P/Ex, U/A, KUB in the Emergency Department, they were followed out patients department of Urology and perfomed Intravenous Pyelogram. The sensitivity and specificity for KUB were 84.5% and 94.0%, respectively. The positive predictive value was 93.8%, the nagative predictive value was 93.8%. Diagnostic accuracy of urolithiasis in our Emergency Department is 89.1%. There was no relationship between urinary occult blood and KUB or IVP. There was no relationship between degree of occult blood and diagnosis of urolithiasis. For differential diagnosis of urolithisis, emergency ultrasound and IVP will be available. So combination of this diagnostic methods will improve accuracy and safty of diagnosis and treatment of urolithiasis.

피하기종,기종격을 동반한 제초제 중독 1례

신준섭,최옥경,정구영 대한응급의학회 1996 대한응급의학회지 Vol.7 No.2

Paraquat(1,1-dimethyl-4,4-bipyridium dichloride) is lethal herbicide and it is thought to act by turning into superoxide and peroxide free radical in the presence of reducing agents. Among the multiple organ failure, the pulmonary complication is always fatal. We present the case of a patient with subcutaneous emphysema and pneumomediastinum without pneumothorax on 6days later after acute paraquat ingestion. A 28 years old female patient was admitted ED at 10:40 on September 8, 1995(Day 1) after ingestion of 10cc-15cc of paraquat (20% solution) at 20:00 on yesterday. By the sodium dithionite test, the presence of paraquat was negative in the urine. She was alert without oropharyngeal ulceration and had stable vital signs. Her hepatic function was moderately deteriorated (SGOT/SGPT 389/867, ALP 233, TB 5.7) and the others laboratory findings were normal limits. She was transferred to ICU and treatment was according to a our protocole(Table 1). On day 6, she complainted numbness on the right lateral neck and respiratory difficulty. The chest X-ray showed subcutaneous emphysema, pneumomediastinum and pneumomediastinum without pneumothorax. Day after day, the size of subcutaneous emphysema and pneumomediastinum were decreased on chest x-ray and it was not found inflammatory signs. The chest CT showed pulmonary fibrosis on the both lower lung field(onday 10) and the esophagography showed no specific findings without dye leakage(on day 14). On day 16, The high resolution chest CT was showed diffuse pulmonary opacity with cystic lesions and peribronchovascular air densities were found with pericardial emphysema and pneumomediastinum. At 4:30 a.m. on day 18, the respiratory arrest was developed and she was expired at 4:35 a.m. under DNR.

The Ubiquitin-like with PHD and Ring Finger Domains 1 (UHRF1)/DNA Methyltransferase 1 (DNMT1) Axis Is a Primary Regulator of Cell Senescence

Jung, Hyun-Jung,Byun, Hae-Ok,Jee, Byul A.,Min, Seongki,Jeoun, Un-woo,Lee, Young-Kyoung,Seo, Yonghak,Woo, Hyun Goo,Yoon, Gyesoon American Society for Biochemistry and Molecular Bi 2017 The Journal of biological chemistry Vol.292 No.9

Interactions of Lysozyme with Phospholipid Liposmes

Jung, Kyoung-Ok,Kim, Mi-Young,Jeong, In-Cheol,Kim, Andre,Kim, Do-Hui,Kang, Shin-Won,Park, Jang-Su Korean Society of Photoscience 2000 Journal of Photosciences Vol.7 No.2

A study of the interaction between lysozyme and phospholipid liposomes is essential for a systematic approach for understanding the action mechanism of lysozyme. So we want to clarify the interactions of lysozyme with phospholipid liposmes. We observed that lysozyme more interacts with negatively charged liposomes than with neutral liposome. More hydroxy groups of the phospholipid was very important on that interactions. We recognized the importance of electrostatic interactions in the process of fusion induced by lysozyme. As indicated by UV/Vis experiments, leakage and fusion are two uncoupled process.