백미와 현미가 Casein 및 Gluten 단백질의 PER에 미치는 효과

권인애,윤성혜,이은경,최미숙 연세대학교 생활과학대학 1989 婦學 Vol.22 No.-

The present studies were designed to observe the effect of polished rice and unpolished rice on the enhancement of protein quality of casein and gluten protein in growing rats(Sprague­Dawley strain). Protein quality was compared and evaluated among experimental groups Ⅰ (casein+polished rice), Ⅱ(gluten+polished rice), Ⅲ(casein+unpolished rice), Ⅳ(gluten+unpolished rice), by measuring Protein Efficiency Ratio(PER) after 25 days of experimental period using growing rats. The protein efficiency ratio is the simplest test for protein quality used widely in comparing nutritive values of proteins in foods. The first limiting amino acids on the basis of the FAO/WHO protein scoring pattern( 1973 ) were threonine and lysine for group Ⅰ,Ⅲ and group Ⅱ,Ⅳ respectively. The PER value of group Ⅲ(casein+unpolished rice )was found to be the highest among all groups tested after 2 weeks of experimental period and the PER value of unpolished rice (group Ⅰ,Ⅲ) was improve greatly even to higher value than that of polished rice (group Ⅱ,Ⅳ). Values of PER were found to be in the order of group Ⅲ >Ⅰ>Ⅳ>Ⅱ at the 21 th day and 25th day of experimental period. It si clear that the effect of unpolished rice on the enhancement of protein guality is greater than that of polished rice in this present study.

Siphonobacter intestinalis sp. nov., a bacterium isolated from the feces of Pseudorhynchus japonicus

Shin Ae Lee,Jeong Myeong Kim,Jae-Hyung Ahn,Jae-Ho Joa,Soo-Jin Kim,Mee-Kyung Sang,Jaekyeong Song,Soon-Wo Kwon,Hang-Yeon Weon 한국미생물학회 2016 The journal of microbiology Vol.54 No.11

Strain 63MJ-2T was isolated from the feces of broad-winged katydid (Pseudorhynchus japonicus) collected in Korea. The 16S rRNA gene sequence of this strain showed the highest sequence similarity with that of Siphonobacter aquaeclarae P2T (96.1%) and had low similarities (below 86.3%) with those of other members of family ‘Flexibacteraceae’. The strain 63MJ-2T is a strictly aerobic, Gram-stain-negative, non-motile, rod-shaped bacterium. The strain grew at 4–35°C (optimum, 25–30°C), pH of 5.0–9.0 (optimum, 6.0–7.0), and 0–2.0% (optimum, 1.0–2.0) (w/v) NaCl. The DNA G+C content of strain 63MJ-2T was 43.5 mol%. The major fatty acids were C16:1 ω5c (42.5%), iso-C17:0 3-OH (18.7%), and summed feature 3 (iso-C15:0 2-OH and/or C16:1 ω7c, 18.0%). The major menaquinone was MK-7 and polar lipids were phosphatidylethanolamine, six unknown aminolipids, and five unknown lipids. Based on the evidence from our polyphasic taxonomic study, we conclude that strain 63MJ-2T should be classified as a novel species of the genus Siphonobacter, and propose the name Siphonobacter intestinalis sp. nov. The type strain is 63MJ-2T (=KACC 18663T =NBRC 111883T).

Complete genome sequence of Brucella anthropi strain T16R-87 isolated from tomato (Solanum lycopersicum L.) rhizosphere

Shin Ae Lee,Mee Kyung Sang,Jaekyeong Song,Soon-Wo Kwon,Hang-Yeon Weon 한국미생물학회 2020 미생물학회지 Vol.56 No.4

Brucella anthropi strain T16R-87 was isolated from the rhizosphere of tomato plants. This bacterium showed plant growthpromoting activity under abiotic and biotic stress conditions, including drought, salinity, and bacterial wilt disease. Its genome consists of two circular chromosomes with 2,645,855 bp and 2,090,924 bp. The genomic G + C content was 55.96%. In total, the genome includes 4,501 genes, 12 rRNAs, and 59 tRNAs. Genes related to antioxidant activity, proline and siderophore biosynthesis, and phosphonate degradation, which may contribute to the promotion of plant growth under environmental stresses, were also found in the genome.

Chryseolinea soli sp. nov., isolated from soil

Shin Ae Lee,Yi-Seul Kim,Mee Kyung Sang,Jaekyeong Song,Soon-Wo Kwon,Hang Yeon Weon 한국미생물학회 2019 The journal of microbiology Vol.57 No.2

A yellow-colored bacterium with gliding motility, strain KIS68-18T, was isolated from a soil sample at Bijin Island in Tongyeong city, Republic of Korea. The cells were strictly aerobic, Gram-staining-negative, non-spore-forming, and rod-shaped. The strain grew at the range of 10–35°C (optimum, 25–30°C), pH 5.5–8.0 (optimum, 6.0–7.5), and 0–0.5% (w/v) NaCl. A phylogenetic analysis based on 16S rRNA gene sequences revealed that strain KIS68-18T was closely related to Chryseolinea serpens DSM 24574T (98.9%) and had low sequence similarities (below 92.6%) with other members of the family ‘Cytophagaceae’ in the phylum Bacteroidetes. The major respiratory quinone system was MK-7 and the predominant cellular fatty acids were C16:1 ω5c (38.8%), iso-C15:0 (18.5%), and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c, 10.6%). The polar lipids consisted of phosphatidylethanolamine, one unidentified phospholipid, three unidentified aminophospholipids, two unidentified aminolipids, and five unidentified lipids. The DNA G + C content was 50.9%. Based on the phylogenetic, physiological, and chemotaxonomic data, stain KIS68-18T represents a novel species of the genus Chryseolinea, for which the name Chryseolinea soli sp. nov. is proposed. The type strain of Chryseolinea soli is KIS68-18T (= KACC 17327T = NBRC 113100T).

Paenibacillus lycopersici sp. nov. and Paenibacillus rhizovicinus sp. nov., isolated from the rhizosphere of tomato (Solanum lycopersicum)

Shin Ae Lee,Tae-Wan Kim,Jun Heo,Mee Kyung Sang,Jaekyeong Song,Soon-Wo Kwon,Hang-Yeon Weon 한국미생물학회 2020 The journal of microbiology Vol.58 No.10

Two Gram-stain-positive, rod-shaped, endospore-forming bacteria, designated 12200R-189T and 14171R-81T were isolated from the rhizosphere of tomato plants. The 16S rRNA gene sequence similarity between strains 12200R-189T and 14171R-81T were 97.2%. Both strains showed the highest 16S rRNA gene sequence similarities to Paenibacillus sacheonensis SY01T (96.3% and 98.0%, respectively). The genome of strain 12200R-189T was approximately 6.7 Mb in size with 5,750 protein-coding genes (CDSs) and the G + C content was 58.1 mol%, whereas that of strain 14171R-81T comprised one chromosome of 7.0 Mb and two plasmids (0.2 Mb each) with 6,595 CDSs and the G + C content was 54.5 mol%. Comparative genome analysis revealed that average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values among 12200R-189T, 14171R-81T, and other closely related species were below the cut-off levels 95% and 70%, respectively. Strain 12200R-189T grew at a temperature range of 15–40°C, pH 6.0–9.0, and 0–3% NaCl (w/v), whereas strain 14171R-81T grew at a temperature range of 10–37°C, pH 6.0– 8.0, and 0–1% NaCl (w/v). Menaquinone-7 (MK-7) was the only isoprenoid quinone detected in both strains. The predominant cellular fatty acids (> 10%) were iso-C15:0, anteiso- C15:0, and iso-C16:0. The polar lipids of strain 12200R- 189T were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), aminophospholipid (APL), phospholipid (PL), phosphatidylglycolipid (PGL), and four aminophosphoglycolipids (APGLs) and those of strain 14171R-81T were DPG, PG, PE, APL, three PLs, two PGLs, and three APGLs. Based on phylogenetic, genomic, phenotypic, and chemotaxonomic analyses, strains 12200R- 189T and 14171R-81T represent two novel species of the genus Paenibacillus, for which the names Paenibacillus lycopersici sp. nov. and Paenibacillus rhizovicinus sp. nov. are proposed. The type strains are 12200R-189T (= KACC 19916T = CCTCC AB 2020027T) and 14171R-81T (= KACC 19915T = CCTCC AB 2020026T).

청바지의 소재별 쾌적감에 관한 연구

이미식,홍문경,권계화,전정애 한국의류학회 2001 한국의류학회지 Vol.25 No.2

The purpose of this study was to compare the comfort sensation depending on four different kinds of denim blue jeans: cotton, cotton/tencel, tencel, cotton/pp. The objective and subjective experiments were conducted to measure the comfort of blue jeans. To investigate the objective comfort, physical properties related to thermal insulation, moisture properties and hand were measured. For subjective comfort measurement, 5 healthy female college students were taken as subjects. The outcomes of the experiments are as follows: The higher the air permeability and bulk density of the denim, the lower the thermal insulation, the thicker the denim, the higher the thermal insulation. Tencel blending denim showed the higher bulk density, the lower air contents, and consequently the lower thermal insulation than the other denims. Tencel showed the highest moisture regain, and cotton/tencel blend showed the highest water vapor permeability. Tencel denim had relatively better flexibility, shape stability and elastic recovery than the other denims. The total hand values of the denims by KES-FB system were not significantly different. Cotton and cotton/pp denims raised the subjects' body temperature after excercise more than tencel or cotton/tencel denims. Average skin temperature was found to have a correlation with micro climate temperature and micro climate humidity. The correlation coefficients were 0.749 and 0.767, respectively. However, average skin temperatures were not significantly different among the materials. Pulse rate was found to be the highest when wearing cotton/pp and the lowest in case of cotton/tencel denim. The energy was consumed in order of cotton > cotton/pp > tencel > cotton/tencel. There was no significant difference in preference before excercise, but, after the excercise, the order of preference changed as the following; cotton/tencel > tencel > cotton/pp > cotton.

국내 상급종합병원 간호부서의 근거기반간호 실태조사

조용애(Young Ae Cho),구미옥(Mee Ock Gu),정재심(Jae Sim Jeong),은영(Young Eun),김신미(Shin Mi Kim),정인숙(Ihn Sook Jung),박명숙(Myung Sook Park),권정순(Jeong Soon Kwon),정영선(Young Sun Jung),김경숙(Kyeongsug Kim),박명화(Myong Hwa Pa 한국근거기반간호학회 2013 근거와 간호 Vol.1 No.1

Purpose: This study was conducted to identify evidence-based nursing (EBN) with directors of nursing and nurses who took full charge of evidence-based practice (EBP) and nursing education at general hospitals in Korea. Methods: Using a questionnaire, the data were collected from 30 nursing directors and 27 nurses responsible for EBP at 30 general hospitals in Korea from August to September, 2013. and analyzed using descriptive statistics. Results: Directors of nursing and those in charge of EBP and nursing education appeared to perceive the importance of EBP, though approximately 60% of general hospitals showed insufficient status for EBN. There were wide differences in EBP educational programs, and EBP training courses, and EBN project implementation in accordance with institutional support. Conclusion: The institutional support should take a leading role in providing nurses with more viable strategies to get EBN settled in clinical nursing settings.

Silorane 기질 복합레진의 색조와 두께가 중합 물성에 미치는 영향

손성애(Sung-Ae Son),노형미(Hyoung Mee Roh),권용훈(Yong Hoon Kwon),진명욱(Myoung-Uk Jin),박정길(Jeong-Kil Park) 대한치과재료학회 2014 대한치과재료학회지 Vol.41 No.4

산업근로자의 스트레스 요인과 증세에 관한 연구

조미애,남철현,이송권,김복용,김귀희,이미경,김중진 慶山大學校 保健福祉硏究所 2001 保健福祉硏究 Vol.6 No.-

This study was conducted to examine causes and symptoms of industrial workers' stress. Data were collected from 869 workers in Daegu, Ulsan, Changwon, and Kyungsan from May 1, 2000 to August 31, 2000. The results of this study are summarized as follows. 1.Among causes of stress, lack of free time marked the lowest points(3.07 ± 1.01 on the basis of 5). The points then were high in order of financial status, living environment, friends, neighbors, relatives, spouse, relationship with the other sex, and family relation. 2.Clerical workers, nighttime workers, workers in large sized industrial places, seated workers, and workers using power of body or shoulders were under more stress than the other workers. 3.The workers who were not satisfied with vocational aptitude or working environments were under high stress level in living environment, financial status, free time, family situation, and relationship with friends, neighbors, relatives, the other sex, or spouse. 4.Smokers, drinkers, workers who used to have coffee or other beverages, workers who did not make efforts to get rid of stress, workers who did not receive education related to stress, and workers who did not obtain informations on stress were under high stress in living environment, financial status, free time, family relation, relationship with friends, neighbors, relatives, spouse, or the other sex. 5.The worker's amount of stress was shown in order of relationship with fellow workers or higher workers, pride, promotion and salary, vocational aptitude, overwork, and working environment. 6.The level of stress symptom was 36.2 points on the basis of 100 points. Among the factors, psychological factor showed the lowest points(30.3), while behavioral factor displayed the highest points(43.2). Physical factor marked 39.9 points. Workers' health is very important for improvement of productivity. The employers have to create harmonious human relationship in the industrial places. They also must make the workers have pride in job and be concerned about promotion and pay, vocational aptitude, and improvement of working environment. The employers, health specialists, and professional counselors must develop comprehensive educational program in order to help industrial workers remove stress.

The effect of resin thickness on polymerization characteristics of silorane-based composite resin

Son, Sung-Ae,Roh, Hyoung-Mee,Hur, Bock,Kwon, Yong-Hoon,Park, Jeong-Kil The Korean Academy of Conservative Dentistry 2014 Restorative Dentistry & Endodontics Vol.39 No.4

Objectives: This study examined the influence of the resin thickness on the polymerization of silorane- and methacrylate-based composites. Materials and Methods: One silorane-based (Filtek P90, 3M ESPE) and two methacrylate-based (Filtek Z250 and Z350, 3M ESPE) composite resins were used. The number of photons were detected using a photodiode detector at the different thicknesses (thickness, 1, 2 and 3 mm) specimens. The microhardness of the top and bottom surfaces was measured (n = 15) using a Vickers hardness with 200 gf load and 15 sec dwell time conditions. The degree of conversion (DC) of the specimens was determined using Fourier transform infrared spectroscopy (FTIR). Scratched powder of each top and bottom surface of the specimen dissolved in ethanol for transmission FTIR spectroscopy. The refractive index was measured using a Abbe-type refractometer. To measure the polymerization shrinkage, a linometer was used. The results were analyzed using two-way ANOVA and Tukey's test at p < 0.05 level. Results: The silorane-based resin composite showed the lowest filler content and light attenuation among the specimens. P90 showed the highest values in the DC and the lowest microhardness at all depth. In the polymerization shrinkage, P90 showed a significantly lower shrinkage than the rest two resin products (p < 0.05). P90 showed a significantly lower refractive index than the remaining two resin products (p < 0.05). Conclusions: DC, microhardness, polymerization rate and refractive index linearly decreased as specimen thickness linearly increased. P90 showed much less polymerization shrinkage compared to other specimens. P90, even though achieved the highest DC, showed the lowest microhardness and refractive index.