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- 검색키워드
- 저자 : Kunisu, Masahiro (검색결과 4 건)
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Niimi, S.,Imoto, M.,Kunisue, T.,Watanabe, M.X.,Kim, E.Y.,Nakayama, K.,Yasunaga, G.,Fujise, Y.,Tanabe, S.,Iwata, H. Academic Press 2014 Ecotoxicology and environmental safety Vol.108 No.-
Hepatic concentrations of persistent organochlorines (OCs) were determined in the common minke whale (Balaenoptera acutorostrata) from the North Pacific. To investigate the effects of OCs on the transcriptome in the minke whale, the present study constructed a hepatic oligo array of this species where 985 unique oligonucleotides were spotted and further analyzed the relationship between the OC levels and gene expression profiles of liver tissues. The stepwise multiple linear regression analysis identified 32 genes that correlated with hepatic OC levels. The mRNA expression levels of seven cytochrome P450 (CYP) genes, CYP1A1, 1A2, 2C78, 2E1, 3A72, 4A35, and 4V6 showed no clear correlations with the concentration of each OC, suggesting that the accumulated OCs in the liver did not reach levels that could alter CYP expression. Among the genes screened by the custom oligo array analysis, hepatic mRNA expression levels of 16 genes were further measured using quantitative real-time reverse transcription polymerase chain reaction. The mRNA levels of vitamin D-binding protein (DBP) were negatively correlated with non-ortho coplanar polychlorinated biphenyl (PCB) levels. Androgen receptor-associated coregulator 70 (ARA70) expression levels showed a significant positive correlation with concentrations of non-ortho coplanar PCB169. These correlations suggest that coplanar PCB-reduced DBP expression could suppress vitamin D receptor-mediated signaling cascades in peripheral tissues. Alternatively, the suppression of vitamin D receptor signaling cascade could be enhanced through competition with the androgen receptor signaling pathway for ARA70. In addition, a negative correlation between kynureninase and PCB169 levels was also observed, which suggest an enhanced accumulation of an endogenous aryl hydrocarbon receptor agonist, kynurenine in the minke whale population. Further studies are necessary to translate the changes in the transcriptome to toxicological outcomes including the disruption of the nervous and immune systems.
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Real-Time, in Situ Monitoring of the Oxidation of Graphite: Lessons Learned
Morimoto, Naoki,Suzuki, Hideyuki,Takeuchi, Yasuo,Kawaguchi, Shogo,Kunisu, Masahiro,Bielawski, Christopher W.,Nishina, Yuta American Chemical Society 2017 Chemistry of materials Vol.29 No.5
<P>Graphite oxide (GO) and its constituent layers (i.e., graphene oxide) display a broad range of functional groups and, as such, have attracted significant attention for use in numerous applications. GO is commonly prepared using the 'Hummers method' or a variant thereof in which graphite is treated with KMnO4 and various additives in H2SO4. Despite its omnipresence, the underlying chemistry of such oxidation reactions is not well understood and typically affords results that are irreproducible and, in some cases, unsafe. To overcome these limitations, the oxidation of graphite under Hummers-type conditions was monitored over time using in situ X-ray diffraction and in situ X-ray absorption near edge structure analyses with synchrotron radiation. In conjunction with other atomic absorption spectroscopy, UV vis spectroscopy and elemental analysis measurements, the underlying mechanism of the oxidation reaction was elucidated, and the reaction conditions were optimized. Ultimately, the methodology for reproducibly preparing GO on large scales using only graphite, H2SO4 and KMnO4 was developed and successfully adapted for use in continuous flow systems.</P>
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Chicken embryo toxic effects of 1,3,7-TriBDD, a naturally produced dioxin
Jae Gon Park(박재곤),Hisato Iwata(이와타 히사토),Nguyen Minh Tue(투 엔구옌 민),Tatsuya Kunisue(쿠니스 타스야),Eun-Young Kim(김은영) 환경독성보건학회 2021 한국독성학회 심포지움 및 학술발표회 Vol.2021 No.5
Dioxins are known to induce a variety of toxic effects and biological alterations as chemical pollutants. Until now, most of dioxins have been reported that they are derived from human activities such as industrial, combustion, and incineration sources. However, recent researches have shown that naturally produced dioxins were detected. 1,3,7-tribromodibenzo-p-dioxin (1,3,7-TriBDD) is one of the natural occurred dioxins by algal species and abundant polybrominated dibenzo-p-dioxins in marine ecosystem. 1,3,7-TriBDD is assimilated in mussels, and ingested by water birds with tons of mussels. Thus, it is possible that 1,3,7-TriBDD could effect on bird species. But, despite this possibility, the effects of biological exposure on vertebrates such as birds are not well known. Therefore, we performed in ovo exposure test and hepatic transcriptomic analysis with chicken embryos to understand effect of 1,3,7-TriBDD exposure. Chicken embryos treated with 27μM and 137μM of 1,3,7-TriBDD and sacrificed after 21 day incubation. Treated chicken embryos showed decreased glucose level in 1,3,7-TriBDD treated embryos. In transcriptomic analysis, 733 and 596 genes were identified as differentially expressed genes (DEGs) from 6520 sequences in 27 and 137μM of 1,3,7-TriBDD-treated groups, respectively. Pathway analysis with DEGs (differentially expressed genes) suggested that 1,3,7-TriBDD possibly induces cancerous effects and alteration of metabolism. And these results were similar with results of TCDD, the representative dioxin, treated chicken embryos. Collectively, this study suggests the necessity to further investigate the effects of 1,3,7-TriBDD exposure in animals, considering a possibility of increased production of 1,3,7-TriBDD due to climate change such as global warming.
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