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RISS 인기검색어
- 검색키워드
- 저자 : Krishna Venkatarangaiah (검색결과 3 건)
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Nagaraja Deeplanaik,Krishna Venkatarangaiah,Ramesh Chapeyil Kumaran,Santosh Kumar Hulikal Shivashankar,Dadakhalandar Doddamani,Sandeep Telkar 한국작물학회 2013 Journal of crop science and biotechnology Vol.16 No.4
Pigeonpea, a drought tolerant, semi-arid pulse crop has been investigated for the expression of differentially expressed genes(DEGs) under drought stress. The cDNA library of soybean leaf tissue retrieved from the Unigene database of the NCBI, were comparedfor in silico expression using IDEG6 web statistical tool. A list of 52 non-redundant DEGs consisting of 11 up-regulated and41 down-regulated was obtained. Among these, more photosynthesis and light harvesting proteins were down-regulated in droughtstress conditions. Pathways were assigned based on KEGG database, revealing 32 genes involved in 17 metabolic pathways. Homologous sequences of six up-regulated genes namely, ADF3, APB, ASR, DLP, LTP1, and UGE5 were then used for quantitativereverse transcription PCR (qRT-PCR) in pigeonpea. The qRT-PCR result revealed the significant up-regulation of dehydrin-like protein(DLP) (5.02 log2 fold) and down-regulation of acid phosphatase class B family protein (APB) (9.43 log2 fold) and non-specificlipid transfer protein 1-like (LTP1) (18.81 log2 fold) in pigeonpea water-stressed leaf sample compared to well-watered leaf samples. No significant difference was observed in the stressed root compared to the stressed pigeonpea leaf sample except that APB showedan up-regulation of 11.35 log2 fold change
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A New Signal Sequence for Recombinant Protein Secretion in Pichia pastoris
( Nagaraj Govindappa ),( Manjunatha Hanumanthappa ),( Krishna Venkatarangaiah ),( Sankar Periyasamy ),( Suma Sreenivas ),( Rajeev Soni ),( Kedarnath Sastry ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.3
Pichia pastoris is one of the most widely used expression systems for the secretory expression of recombinant proteins. The secretory expression in P. pastoris usually makes use of the prepro MATα sequence from Saccharomyces cerevisiae, which has a dibasic amino acid cleavage site at the end of the signal sequence. This is efficiently processed by Kex2 protease, resulting in the secretion of high levels of proteins to the medium. However, the proteins that are having the internal accessible dibasic amino acids such as KR and RR in the coding region cannot be expressed using this signal sequence, as the protein will be fragmented. We have identified a new signal sequence of 18 amino acids from a P. pastoris protein that can secrete proteins to the medium efficiently. The PMT1-gene-inactivated P. pastoris strain secretes a ~30 kDa protein into the extracellular medium. We have identified this protein by determining its N-terminal amino acid sequence. The protein secreted has four DDDK concatameric internal repeats. This protein was not secreted in the wild-type P. pastoris under normal culture conditions. We show that the 18-amino-acid signal peptide at the N-terminal of this protein is useful for secretion of heterologous proteins in Pichia.
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Deeplanaik, Nagaraja,Kumaran, Ramesh Chapeyil,Venkatarangaiah, Krishna,Shivashankar, Santosh Kumar Hulikal,Doddamani, Dadakhalandar,Telkar, Sandeep 한국작물학회 2013 Journal of crop science and biotechnology Vol.16 No.4
Pigeonpea, a drought tolerant, semi-arid pulse crop has been investigated for the expression of differentially expressed genes (DEGs) under drought stress. The cDNA library of soybean leaf tissue retrieved from the Unigene database of the NCBI, were compared for in silico expression using IDEG6 web statistical tool. A list of 52 non-redundant DEGs consisting of 11 up-regulated and 41 down-regulated was obtained. Among these, more photosynthesis and light harvesting proteins were down-regulated in drought stress conditions. Pathways were assigned based on KEGG database, revealing 32 genes involved in 17 metabolic pathways. Homologous sequences of six up-regulated genes namely, ADF3, APB, ASR, DLP, LTP1, and UGE5 were then used for quantitative reverse transcription PCR (qRT-PCR) in pigeonpea. The qRT-PCR result revealed the significant up-regulation of dehydrin-like protein (DLP) (5.02 log2 fold) and down-regulation of acid phosphatase class B family protein (APB) (9.43 log2 fold) and non-specific lipid transfer protein 1-like (LTP1) (18.81 log2 fold) in pigeonpea water-stressed leaf sample compared to well-watered leaf samples. No significant difference was observed in the stressed root compared to the stressed pigeonpea leaf sample except that APB showed an up-regulation of 11.35 log2 fold change.
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