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RISS 인기검색어
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- 저자 : Kotaro Onishi (검색결과 2 건)
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( Mikiya Araki ),( Kotaro Oki ),( Takashi Onishi ),( Seiichi Shiga ) 한국액체미립화학회 2013 한국액체미립화학회 학술강연회 논문집 Vol.2013 No.-
Feasibility of a newly developed non-intrusive particle size measurement method in sub-micron region using multi-wavelength polarized lights is investigated, experimentally. Figure 1 shows the optical setup. Polystyrene particles are used as the test particles, which are suspended in purified water. The arithmetic mean diameters of them are varied from 0.356 to 3.00 μm, which are previously measured with a conventional method (centrifugal sedimentation method). The laser beam passes through the test particles, and the scattered lights are acquired with a digital CCD camera set in parallel and perpendicular directions to the polarization plane of the incident light. Based on the Mie`s scattering theory, the ratio of scattered lights for these two components is given as a function of the particle diameter, the refractive index, and the wavelength of the incident light. By comparing the polarization ratio calculated analytically with those obtained experimentally, the particle size can be determined, which is called polarization ratio method. The major drawback of this method is the narrow measurement range, typically up to the order of 0.1 μm, which is attributed to the complicated behavior of the polarization ratio in the Mie scattering region. In order to extend the measurement range of conventional polarization ratio methods, three kinds of He-Ne lasers, whose wavelengths are 543.5, 594.1, and 632.8 nm, are used. The polarization ratios of the scattered lights are obtained from these scattered light images, and the acquired data are compared with the analytical values. Figure 2 shows the data comparison with a conventional method. It is shown that, by use of the multiwavelength polarization ratio method, the measurement range of the particle size can be extended up to the order of 3 μm, and that the accuracy of the results lies from 0.1 % to 7.6 %, when compared with the results of a centrifugal sedimentation method.
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Molecular Identification of Oesophagostomum and Trichuris Eggs Isolated from Wild Japanese Macaques
Naoki Arizono,Minoru Yamada,Tatsuya Tegoshi,Kotaro Onishi 대한기생충학열대의학회 2012 The Korean Journal of Parasitology Vol.50 No.3
Natural habitat fragmentation and reducing habitat quality have resulted in an increased appearance of Japanese macaques, Macaca fuscata (Gray, 1870), in suburban areas in Japan. To investigate the risk of zoonotic infections, a coprological survey of helminth eggs passed by wild Japanese macaques was carried out in 2009 and 2010 in Shiga Prefecture, Japan. Microscopic examination found helminth eggs in high prevalence, and nucleotide sequencing of DNA extracted from the eggs identified Oesophagostomum cf. aculeatum and Trichuris trichiura. A fecal culture also detected infective larvae of Strongyloides fuelleborni. These zoonotic nematodes pose a potential health issue to local people in areas frequented by Japanese macaques.
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