A New Pea Cultivar, Cheongmi with Short Stem Height, Lodging Resistance and High Yield

Won Young Han,Sang Ouk Shin,Myoung Gun Choung,Chan Sik Jung,Doo Chull Shin,Sea Kwan Oh,Sung Taeg Kang,In Youl Baek,Duck Yong Suh,Soon Chul Kim,Dong Kwan Kim,Chang Ki Son 한국육종학회 2004 한국육종학회지 Vol.36 No.5

A new pea cultivar, Cheongmi was developed at the Yeongnam Agricultural Research Institute (YARI) in 2003. It was selected from a cross YP303 (Frescoloy / Upton) // YP113 (Sparkle/Early Bird) /3/ YP115 (Sparkle / Euiseongjaerae) in 1992. The preliminary,

Poster Exhibition : Comparison of Surrogate Serum Markers and Transient Elastography (Fibroscan) for Assessing Cirrhosis in Patients with Chronic Viral Hepatitis (초)

Myoung Hee Lee,Jae Youn Cheong,Soon Ho Um,Yeon Seok Seo,Dong Joon Kim,Seong Gyu Hwang,Jin Mo Yang,Kwqn Hyub Han,Sung Won Cho 대한간학회 2009 Clinical and Molecular Hepatology(대한간학회지) Vol.15 No.4(S)

Single-Factor SOX2 Mediates Direct Neural Reprogramming of Human Mesenchymal Stem Cells via Transfection of <i>In Vitro</i> Transcribed mRNA

Kim, Bo-Eun,Choi, Soon Won,Shin, Ji-Hee,Kim, Jae-Jun,Kang, Insung,Lee, Byung-Chul,Lee, Jin Young,Kook, Myoung Geun,Kang, Kyung-Sun Cognizant Communication Corp. 2018 CELL TRANSPLANTATION Vol. No.

<P>Neural stem cells (NSCs) are a prominent cell source for understanding neural pathogenesis and for developing therapeutic applications to treat neurodegenerative disease because of their regenerative capacity and multipotency. Recently, a variety of cellular reprogramming technologies have been developed to facilitate <I>in vitro</I> generation of NSCs, called induced NSCs (iNSCs). However, the genetic safety aspects of established virus-based reprogramming methods have been considered, and non-integrating reprogramming methods have been developed. Reprogramming with <I>in vitro</I> transcribed (IVT) mRNA is one of the genetically safe reprogramming methods because exogenous mRNA temporally exists in the cell and is not integrated into the chromosome. Here, we successfully generated expandable iNSCs from human umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) via transfection with IVT mRNA encoding SOX2 (SOX2 mRNA) with properly optimized conditions. We confirmed that generated human UCB-MSC-derived iNSCs (UM-iNSCs) possess characteristics of NSCs, including multipotency and self-renewal capacity. Additionally, we transfected human dermal fibroblasts (HDFs) with SOX2 mRNA. Compared with human embryonic stem cell-derived NSCs, HDFs transfected with SOX2 mRNA exhibited neural reprogramming with similar morphologies and NSC-enriched mRNA levels, but they showed limited proliferation ability. Our results demonstrated that human UCB-MSCs can be used for direct reprogramming into NSCs through transfection with IVT mRNA encoding a single factor, which provides an integration-free reprogramming tool for future therapeutic application.</P>

A Quantiative Comparison of Vaccinia virus Shedding from Conventional Dressing Sites and Vaccination Lesions after Smallpox Vaccination

Kim, Sung-Han,Yeo, Sang-Gu,Cho, Jae-Hyun,Bang, Ji-Whan,Kim, Hong-Bin,Kim, Nam-Joong,Jee, Youngmee,Cho, Haewol,Oh, Myoung-don,Choe, Kang-Won 대한감염학회 2007 감염과 화학요법 Vol.39 No.2

목적 : 두창백신 접종 후 일반적으로 접종 부위에 거즈를 먼저 덮고, 그 위에 반투과막을 덮어주는 드레싱을 권장하고 있다. 저자들은 이러한 드레싱 방법의 효율성을 알아보기 위해서 두창백신 접종 후 접종 부위, 거즈 바깥부위, 반투과막 바깥 부위에서 각각 분비되는 바이러스양을 정량적으로 비교하였다. 재료 및 방법 : Lancy-Vaxina^((R)) (Berna Biotech, Swiss) 두창백신 1:10 희석연구에 참여한 자원자들 중에서 접종부위에 정해진 기간에 검체 채취에 동의한 40명을 대상으로 연구를 진행하였다. 참가자들은 두창백신 접종 후 6, 8, 10, 13, 15일에 각각 방문하여 드레싱을 교체하였다. 드레싱을 교체하면서 두창백신 접종 부위, 거즈 바깥부위, 반투과막 바깥부위에 면봉을 이용해서 각각 검체를 채취하였다. 검체는 냉동 보관한 후 plaque assay를 통하여 정량적으로 바이러스 역가를 측정하였다. 결과 : 총 40명의 자원자가 본 연구에 참가하였다. 백신 접종 부위에서 채취한 156 검체 중 126건(81%)에서 백시니아바이러스가 검출되었다. 백신 접종 부위에서 접종 8일째 바이러스 역가의 평균(geometric mean titer(log_(10))은 3.91 pfu/mL이었다. 거즈 바깥부위에서 총 39검체가 얻어졌고, 이 중 16건(41%)에서 백시니아바이러스가 검출되었다. 거즈 바깥부위에서 접종 8일째 바이러스 역가의 평균은 0.91 pfu/mL이었다. 반투과막 바깥부위에서 총 133 검체가 얻어졌고, 이 중 1건(0.8%)에서 백시니아바이러스가 검출되었다. 접종 8일째 거즈 바깥부위에서는 바이러스가 검출되지 않았다. 결론 : 두창백신 접종 후에 거즈를 덮고 그 위에 반투과막을 덮어주는 드레싱 방법은 백시니아바이러스 배출을 효과적으로 줄여줄 수 있음을 확인하였다. Background : We compared vaccinia virus shedding from the vaccine inoculation site (vaccination lesion) and two sites of a dressing covering the vaccination site; the outer surface of the semipermeable dressing (outer surface) and the inner surface of the semipermeable dressing, that is, the surface of a folded gauze under the semipermeable membrane (gauze surface) Material and Methods : Subjects were recruited from the volunteers who participated in a clinical trial of the efficacy of a 1:10 dilution of Lancy-Vaxina^((R)) (Berna Biotech, Switzerland), and were seen every 2-3 days (days 6, 8, 10, 13, and 15 after smallpox vaccination) for scheduled dressing changes. Swab specimens were obtained from the vaccination lesion, the outer surface, and the gauze surface. Quantitative viral culture assays for these specimens were done. Results : Vaccinia virus was recovered from 126 (81%) of the 156 vaccination lesion samples collected from the 40 participants. A high virus titer was recovered from the vaccination lesion (geometric mean titer (log_(10))=3.91 on day 8). Of the 39 swab samples obtained from the gauze surface of the gauze, 16 (41%) were positive for virus. An intermediate titer was recovered from the gauze surface (geometric mean titer (log_(10))=0.91 on day 8). Of the 133 swab samples obtained from the outer surface, only one (0.8%) was positive for vaccinia. No virus was recovered from the outer surface on day 8. Conclusion : Our findings suggest that the addition of a semipermeable dressing to the folded gauze further reduces viral shedding and therefore increases protection.

Diagnosis of Central Nervous System Tuberculosis by T-Cell-Based Assays on Peripheral Blood and Cerebrospinal Fluid Mononuclear Cells

Kim, Sung-Han,Chu, Kon,Choi, Su-Jin,Song, Kyoung-Ho,Kim, Hong-Bin,Kim, Nam-Joong,Park, Seong-Ho,Yoon, Byung-Woo,Oh, Myoung-don,Choe, Kang-Won American Society for Microbiology 2008 CLINICAL AND VACCINE IMMUNOLOGY Vol.15 No.9

<B>ABSTRACT</B><P>In active tuberculosis (TB), <I>Mycobacterium tuberculosis</I>-specific T cells are compartmentalized more to the site of infection than to the circulating blood. Therefore, an <I>M. tuberculosis</I>-specific enzyme-linked immunospot (ELISPOT) assay with samples from the site of infection may permit a more sensitive or specific diagnosis of active central nervous system (CNS) TB than that achieved by the assay with blood alone. Therefore, we prospectively evaluated the usefulness of circulating and compartmentalized mononuclear cell (MC; i.e., peripheral blood mononuclear cell [PBMC] and cerebrospinal fluid [CSF] MC)-based ELISPOT assays (i.e., the T-SPOT.TB test) for the diagnosis of active TB in patients with suspected CNS TB. The clinical categories of CNS TB were classified as described previously (G. E. Thwaites, T. T. Chau, K. Stepniewska, N. H. Phu, L. V. Chuong, D. X. Sinh, N. J. White, C. M. Parry, and J. J. Farrar, Lancet 360:1287-1292, 2002). Thirty-seven patients with suspected CNS TB were enrolled over a 12-month period. Of these, 31 (84%) showed clinical manifestations of suspected TB meningitis and 6 (16%) gave indications of intracranial tuberculoma with disseminated TB. The final clinical categories of the 37 patients with suspected CNS TB were as follows: 12 (32%) were classified as having CNS TB (7 with confirmed TB, 3 with probable TB, and 2 with possible TB) and 25 (68%) were classified as not having active TB. The sensitivity and specificity of the PBMC ELISPOT assay were 91% (95% confidence interval [CI], 59% to 100%) and 63% (95% CI, 41% to 81%), respectively. By comparison, the sensitivity and specificity of the CSF MC ELISPOT assay were 75% (95% CI, 19% to 99%) and 75% (95% CI, 43% to 95%), respectively. When the ratio of the CSF MC ELISPOT assay results to the PBMC ELISPOT results was 2 or more, the sensitivity and specificity were 50% (95% CI, 7% to 93%) and 100% (95% CI, 74% to 100%), respectively. The ELISPOT assay with PBMCs and CSF MCs is a useful adjunct to the current tests for the diagnosis of CNS TB.</P>

Sequential spinal and intracranial dural metastases in gastric adenocarcinoma: A case report

Kim, Hongsik,Yi, Kyung Sik,Kim, Won-Dong,Son, Seung-Myoung,Yang, Yaewon,Kwon, Jihyun,Han, Hye Sook Baishideng Publishing Group Inc 2018 WORLD JOURNAL OF GASTROENTEROLOGY Vol.24 No.5

<P>Dural metastasis from primary gastric adenocarcinoma has been rarely reported, and its prognosis is very poor because it frequently leads to acute subdural hematoma. Here, we describe a case with sequential spinal and cranial dural metastases from gastric adenocarcinoma without subdural hematoma. A 43-year-old woman with gastric adenocarcinoma and well-controlled peritoneal carcinomatosis presented with back pain, right radiating leg pain, left facial palsy, and hearing loss. Magnetic resonance imaging of the spine and brain revealed dural masses at the lumbosacral junction with invasion to the L5 and S1 nerve roots and at the skull base with invasion to the internal auditory canal. She was treated with local radiotherapy, and her pain and neurologic symptoms improved after palliative radiotherapy. This is the first reported case of dural metastases of gastric adenocarcinoma of the spine and skull base but with a relatively indolent course and without subdural hematoma.</P>

TRANCE, a TNF Family Cytokine, Promotes Angiogenesis : Its angiogenic Action and Signaling Mechanism in Human Endothelial cells

Kim, Young-Mi,Kim, Young-Myoung,Lee, You Mie,Kim, Hae-Sun,Choi, Yongwon,Kim, Kyu-Won,Lee, Soo-Young,Kwon, Young-Guen 이화여자대학교 세포신호전달연구센터 2001 고사리 세포신호전달 심포지움 Vol. No.3

TNF-related activation-induced cytokine(TRANCE), a member of the TNF family, is highly expressed on osteoblasts and plays a key role in the control of bone development. Because angiogenesis is the crucial first step in the processes of proper bone formation, we tested the hypothesis that TRANCE is able to induce the growth of new blood vessels. TRANCE evoked a time- and dose-dependent activation of the mitogen-activated protein kinases ERK1/2, AKT and NF-B in human umbilical vein endothelial cells(HUVECs). TRANCE stimulated in vitro DNA synthesis, chemotactic motility, and capillary-like tube formation of HUVECs. Both Matrigel plug assay in mice and chick chorioallantoic membrane(CAM) assay revealed that TRANCE potently induced neovascularization in vivo. These results indicate that TRANCE, presumably via activation of the endothelial TRANCE receptor, stimulates various angiogenic signaling pathways in endothelial cells. Thus, TRANCE may act as an important modulator of angiogenesis under the physiological and pathological conditions.

Cloning and Expression of Human Clotting Factor 9 cDNA in Escherichia coli

Kim,Myoung-Hee,Hur,Hyang-Suk,Lee,Young-Won THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1996 Journal of biomedical laboratory sciences Vol.2 No.2

인체 혈액 응고 9인자는 간에서 생성되며 461개의 아미노산으로 구성된 당단백질이다. 따라서 인체 혈액응고 9인자 cDNA를 찾기 위해 태아의 간(fetal liver) cDNA library를 PCR(Polymerase Chain reaction) 방법으로 screening 하였으며, 그 결과 ATG개시 코돈으로부터 TAA 종료 코돈까지 포함하는 1.4 kb의 9인자 cDNA를 찾았다. 또한 클론된 9인자 cDNA를 박테리아에서 발현시키기 위해 박테리아 발현 벡터인 pGEX-2T 플라스미드에 클로닝하므로써 pGEX-F9 플라스미드를 제조하였다. pGEX-F9로 형질전환된 E. coli에서 pGEX-F9의 발현을 유도하면 73 kDa 크기의 GST-factor9 융합 단백질이 다량 생성되며, 이 단백질이 혈액 응고 9인자 단백질을 함유하는 융합 단백질임을 혈액 응고 9인자 항체를 이용한 Western bolt으로 입증하였다. E. coli에서 발현된 GST-factor 9 융합단백질은 전체 단백질의 약 20%를 차지하며, GST agarose bead를 이용한 one step purificarion 방법을 통해 GST-factor9 융합 단백질을 쉽게 분리할 수 있다. Human blood clotting (coagulation) factor 9 cDNA which codes for 461 amino acid has been cloned by screening human fetal liver cDNA library using PCR. This 1.4 kb cDNA spanning from the ATG initiation codon to the TAA termination codon was cloned into bacterial expression vector pGEX-2T, generating pGEX-F9 plasmid. The plasmid pGEX-F9 expresses about 73 kDa GST (Glutathione S-transferase)-Factor 9 fusion protein when introduced into E. coli. Western blot analysis using polyclonal antibody raised against human factor 9 confirmed this fusion protein contains factor 9 protein. The level of GST-factor 9 expression was about 20% of total protein and the purification of fusion protein was efficiently achieved by using GST agarose bead based on one step purification protocol.

Molecular and Structural Characterization of the Tegumental 20.6-kDa Protein in <i>Clonorchis sinensis</i> as a Potential Druggable Target

Kim, Yu-Jung,Yoo, Won Gi,Lee, Myoung-Ro,Kang, Jung-Mi,Na, Byoung-Kuk,Cho, Shin-Hyeong,Park, Mi-Yeoun,Ju, Jung-Won MDPI 2017 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.18 No.3

<P>The tegument, representing the membrane-bound outer surface of platyhelminth parasites, plays an important role for the regulation of the host immune response and parasite survival. A comprehensive understanding of tegumental proteins can provide drug candidates for use against helminth-associated diseases, such as clonorchiasis caused by the liver fluke <I>Clonorchis sinensis</I>. However, little is known regarding the physicochemical properties of <I>C. sinensis</I> teguments. In this study, a novel 20.6-kDa tegumental protein of the <I>C. sinensis</I> adult worm (CsTegu20.6) was identified and characterized by molecular and in silico methods. The complete coding sequence of 525 bp was derived from cDNA clones and encodes a protein of 175 amino acids. Homology search using BLASTX showed CsTegu20.6 identity ranging from 29% to 39% with previously-known tegumental proteins in <I>C. sinensis</I>. Domain analysis indicated the presence of a calcium-binding EF-hand domain containing a basic helix-loop-helix structure and a dynein light chain domain exhibiting a ferredoxin fold. We used a modified method to obtain the accurate tertiary structure of the CsTegu20.6 protein because of the unavailability of appropriate templates. The CsTegu20.6 protein sequence was split into two domains based on the disordered region, and then, the structure of each domain was modeled using I-TASSER. A final full-length structure was obtained by combining two structures and refining the whole structure. A refined CsTegu20.6 structure was used to identify a potential CsTegu20.6 inhibitor based on protein structure-compound interaction analysis. The recombinant proteins were expressed in <I>Escherichia coli</I> and purified by nickel-nitrilotriacetic acid affinity chromatography. In <I>C. sinensis</I>, CsTegu20.6 mRNAs were abundant in adult and metacercariae, but not in the egg. Immunohistochemistry revealed that CsTegu20.6 localized to the surface of the tegument in the adult fluke. Collectively, our results contribute to a better understanding of the structural and functional characteristics of CsTegu20.6 and homologs of flukes. One compound is proposed as a putative inhibitor of CsTegu20.6 to facilitate further studies for anthelmintics.</P>

Scalable Solvothermal Synthesis of Superparamagnetic Fe₃O₄ Nanoclusters for Bioseparation and Theragnostic Probes

Kim, Jeonghyo,Tran, Van Tan,Oh, Sangjin,Kim, Chang-Seok,Hong, Jong Chul,Kim, SungIl,Joo, Young-Seon,Mun, Saem,Kim, Myoung-Ho,Jung, Jae-Wan,Lee, Jiyoung,Kang, Yong Seok,Koo, Ja-Won,Lee, Jaebeom American Chemical Society 2018 ACS APPLIED MATERIALS & INTERFACES Vol.10 No.49

<P>Magnetic nanoparticles have had a significant impact on a wide range of advanced applications in the academic and industrial fields. In particular, in nanomedicine, the nanoparticles require specific properties, including hydrophilic behavior, uniform and tunable dimensions, and good magnetic properties, which are still challenging to achieve by industrial-scale synthesis. Here, we report a gram-scale synthesis of hydrophilic magnetic nanoclusters based on a one-pot solvothermal system. Using this approach, we achieved the nanoclusters with controlled size composed of magnetite nanocrystals in close-packed superstructures that exhibited hydrophilicity, superparamagnetism, high magnetization, and colloidal stability. The proposed solvothermal method is found to be highly suitable for synthesizing industrial quantities (gram-per-batch level) of magnetic spheres with unchanged structural and magnetic properties. Furthermore, coating the magnetic spheres with an additional silica layer provided further stability and specific functionalities favorable for biological applications. Using in vitro and in vivo studies, we successfully demonstrated both positive and negative separation and the use of the magnetic nanoclusters as a theragnostic nanoprobe. This scalable synthetic procedure is expected to be highly suitable for widespread use in biomedical, energy storage, photonics, and catalysis fields, among others.</P> [FIG OMISSION]</BR>