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Hedgehog signaling between cancer cells and hepatic stellate cells in promoting cholangiocarcinoma.
Kim, Younjoo,Kim, Myeong-Ok,Shin, Jin Sun,Park, Sun Hoo,Kim, Sang Bum,Kim, Jin,Park, Su Cheol,Han, Chul Ju,Ryu, Ji Kon,Yoon, Yong Bum,Kim, Yong-Tae Raven Press 2014 Annals of Surgical Oncology Vol.21 No.8
<P>Aberrant Hedgehog (HH) signaling activation is important in cancer growth and mediates the interaction between cancer cells and the surrounding stromal cells. This study investigated the role of HH signaling on the growth of cholangiocarcinoma (CC), focusing on the interaction of CC cells with stromal cells.</P>
RIBOSOME-INACTIVATING PROTEIN GENES FOR USE AS ANTIFUNGAL AGENTS IN TRANSGENIC RICE
Kim, Ju-Kon,Wu, Ray,Hwang, Young-Soo Plant molecular biology and biotechnology research 1994 Proceedings the 2nd Korean-Germany joint symposium Vol.1994 No.-
Ribosome-inactivating proteins (RIPs) are known to have cytotoxic activity on eucaryotic cells by cleaving a specific adenine residue of 28S rRNA. To test if RIP can be used as antifungal agents in transgenic rice, two chimeric genes containing the maize ribosome-inactivating protein gene, Zmcrip3a, under the control of the rice Actl (pARP7) and the rbcS promoters (pBY605RR) were introduced into Oryza sativa cv Nipponbare by using particle bombardment. After selection of phosphinotricin-resistant calli, regeneration into plants, and genomic DNA gel-blot analysis of the primary transformants, thirteen and seventeen independent fertile transgenic plants for the pARP7 and the pBY605RR constructs, respectively, were obtained. Immunoblot analysis using an anti-maize RIP polyclonal antibody showed that all of the plants analyzed contain the expected size (34 kDa) of the expressed RIP protein and the amount of the expressed protein is approximately 0.5% of total soluble protein in the leaves of the best expressing transgenic plants.
Kim, Hack-Youn,Kim, Kon-Joong,Lee, Jong-Wan,Kim, Gye-Woong,Choe, Ju-Hui,Kim, Hyun-Wook,Yoon, Yohan,Kim, Cheon-Jei Korean Society for Food Science of Animal Resource 2015 한국축산식품학회지 Vol.35 No.1
The aim of this study was to investigate the effects of various marination processes on the quality characteristics of chicken breast prepared with chicken feet gelatin and wheat fiber. The chicken feet gelatin was swollen with hydrochloric solution (0.1 N HCl, pH $1.31{\pm}0.02$) and dehydrated by freeze-drying. The composition (w/w) of the marinade was water (10%), soy sauce (12%), phosphate (0.3%), wheat fiber (1.5%), and chicken feet gelatin (1.5%). Three samples of chicken breast were manufactured with Tumbler (only tumbler), Tenderizer (tenderizer and tumbler), and Injector (injector and tumbler). The water content of the Injector sample was significantly higher than those of the Tumbler and Tenderizer samples (p<0.05). During heating, the lightness of all chicken breasts increased and the redness decreased. The tumbling and cooking yield of the Injector sample were significantly higher than those of the Tumbler and Tenderizer samples (p<0.05). The shear force of the Tenderizer sample was significantly lower than that of the Tumbler and Injector samples (p<0.05). No significant differences, except for color, were observed in the sensory analysis of the samples. Thus, the proper selection of mechanical processing is important to improve the quality characteristics of marinated chicken breast, considering the types of final products.
Kim, Ju-Myung,Park, Han-Saem,Park, Jang-Hoon,Kim, Tae-Hee,Song, Hyun-Kon,Lee, Sang-Young American Chemical Society 2014 ACS APPLIED MATERIALS & INTERFACES Vol.6 No.15
<P>Rapid growth of mobile and even wearable electronics is in pursuit of high-energy-density lithium-ion batteries. One simple and facile way to achieve this goal is the elimination of nonelectroactive components of electrodes such as binders and conductive agents. Here, we present a new concept of monocomponent electrodes comprising solely electroactive materials that are wrapped with an insignificant amount (less than 0.4 wt %) of conducting polymer (PEDOT:PSS or poly(3,4-ethylenedioxythiophene) doped with poly(styrenesulfonate)). The PEDOT:PSS as an ultraskinny surface layer on electroactive materials (LiCoO<SUB>2</SUB> (LCO) powders are chosen as a model system to explore feasibility of this new concept) successfully acts as a kind of binder as well as mixed (both electrically and ionically) conductive film, playing a key role in enabling the monocomponent electrode. The electric conductivity of the monocomponent LCO cathode is controlled by simply varying the PSS content and also the structural conformation (benzoid-favoring coil structure and quinoid-favoring linear or extended coil structure) of PEDOT in the PEDOT:PSS skin. Notably, a substantial increase in the mass-loading density of the LCO cathode is realized with the PEDOT:PSS skin without sacrificing electronic/ionic transport pathways. We envisage that the PEDOT:PSS-skinned electrode strategy opens a scalable and versatile route for making practically meaningful binder-/conductive agent-free (monocomponent) electrodes.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2014/aamick.2014.6.issue-15/am502736m/production/images/medium/am-2014-02736m_0008.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/am502736m'>ACS Electronic Supporting Info</A></P>
Kim, Beom-Su,Park, Sun-Ju,Kim, Myung-Kon,Kim, Young-Hoi,Lee, Sang-Bong,Lee, Kwang-Hee,Choi, Na-Young,Lee, Young-Rae,Lee, Young-Eun,You, Yong-Ouk Hindawi Publishing Corporation 2015 Evidence-based Complementary and Alternative Medic Vol.2015 No.-
<P>The aim of the study was to evaluate the antibacterial activity of essential oil extracted from <I>Chrysanthemum boreale</I> (<I>C. boreale</I>) on <I>Streptococcus mutans</I> (<I>S. mutans</I>). To investigate anticariogenic properties, and bacterial growth, acid production, biofilm formation, bacterial adherence of <I>S. mutans</I> were evaluated. Then gene expression of several virulence factors was also evaluated. <I>C. boreale</I> essential oil exhibited significant inhibition of bacterial growth, adherence capacity, and acid production of <I>S. mutans</I> at concentrations 0.1–0.5 mg/mL and 0.25–0.5 mg/mL, respectively. The safranin staining and scanning electron microscopy results showed that the biofilm formation was also inhibited. The result of live/dead staining showed the bactericidal effect. Furthermore, real-time PCR analysis showed that the gene expression of some virulence factors such as <I>gtf</I>B, <I>gtf</I>C, <I>gtf</I>D, <I>gbp</I>B, <I>spa</I>P, <I>brp</I>A, <I>rel</I>A, and <I>vic</I>R of <I>S. mutans</I> was significantly decreased in a dose dependent manner. In GC and GC-MS analysis, seventy-two compounds were identified in the oil, representing 85.42% of the total oil. The major components were camphor (20.89%), <I>β</I>-caryophyllene (5.71%), <I>α</I>-thujone (5.46%), piperitone (5.27%), <I>epi</I>-sesquiphellandrene (5.16%), <I>α</I>-pinene (4.97%), 1,8-cineole (4.52%), <I>β</I>-pinene (4.45%), and camphene (4.19%). These results suggest that <I>C. boreale</I> essential oil may inhibit growth, adhesion, acid tolerance, and biofilm formation of <I>S. mutans</I> through the partial inhibition of several of these virulence factors.</P>
Kim, Eun Hye,Kim, Youn Shic,Park, Su-Hyun,Koo, Yeon Jong,Choi, Yang Do,Chung, Yong-Yoon,Lee, In-Jung,Kim, Ju-Kon American Society of Plant Physiologists 2009 Plant Physiology Vol.149 No.4
<P>Jasmonic acid (JA) is involved in plant development and the defense response. Transgenic overexpression of the Arabidopsis (Arabidopsis thaliana) jasmonic acid carboxyl methyltransferase gene (AtJMT) linked to the Ubi1 promoter increased levels of methyl jasmonate (MeJA) by 6-fold in young panicles. Grain yield was greatly reduced in Ubi1:AtJMT plants due to a lower numbers of spikelets and lower filling rates than were observed for nontransgenic (NT) controls. Ubi1:AtJMT plants had altered numbers of spikelet organs, including the lemma/palea, lodicule, anther, and pistil. The loss of grain yield and alteration in spikelet organ numbers were reproduced by treating NT plants with exogenous MeJA, indicating that increased levels of MeJA in Ubi1:AtJMT panicles inhibited spikelet development. Interestingly, MeJA levels were increased by 19-fold in young NT panicles upon exposure to drought conditions, resulting in a loss of grain yield that was similar to that observed in Ubi1:AtJMT plants. Levels of abscisic acid (ABA) were increased by 1.9- and 1.4-fold in Ubi1:AtJMT and drought-treated NT panicles, respectively. The ABA increase in Ubi1:AtJMT panicles grown in nondrought conditions suggests that MeJA, rather than drought stress, induces ABA biosynthesis under drought conditions. Using microarray and quantitative polymerase chain reaction analyses, we identified seven genes that were regulated in both Ubi1:AtJMT and drought-treated NT panicles. Two genes, OsJMT1 and OsSDR (for short-chain alcohol dehydrogenase), are involved in MeJA and ABA biosynthesis, respectively, in rice (Oryza sativa). Overall, our results suggest that plants produce MeJA during drought stress, which in turn stimulates the production of ABA, together leading to a loss of grain yield.</P>