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Hedgehog signaling between cancer cells and hepatic stellate cells in promoting cholangiocarcinoma.
Kim, Younjoo,Kim, Myeong-Ok,Shin, Jin Sun,Park, Sun Hoo,Kim, Sang Bum,Kim, Jin,Park, Su Cheol,Han, Chul Ju,Ryu, Ji Kon,Yoon, Yong Bum,Kim, Yong-Tae Raven Press 2014 Annals of Surgical Oncology Vol.21 No.8
<P>Aberrant Hedgehog (HH) signaling activation is important in cancer growth and mediates the interaction between cancer cells and the surrounding stromal cells. This study investigated the role of HH signaling on the growth of cholangiocarcinoma (CC), focusing on the interaction of CC cells with stromal cells.</P>
Kim, Yeon-Ki,Kim, Jeong-Sook,Cheong, Pil-Joong,Kim, Min-Jeong,Lee, Tae-Ho,Joo, Joung-Su,Kim, Ju-Kon,Nahm, Baek-Hie,Song, Sang-Ik The Korean Society for Applied Biological Chemistr 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.3
Designing high density DNA arrays representing all the genes of an organism could be limited when the entire genome sequence of the organism is not available or if only a limited number of ESTs are available. In an effort to prepare coding sequences as DNA sources for a microarray, we found that coding region enriched genomic DNA libraries can be produced by S1 nuclease treatment of partially denatured genomic DNA. Sequence analysis of about 1,000 clones using BLASTN and BLASTX searches showed that 46% of the clones in the library have regions which share significant similarity with sequences deposited in the rice EST and nr data bases. These data suggested that clones produced in this library could be directly used as PCR templates, where the resulting PCR products could be spotted on slides for microarray analysis. This technique might be applicable in designing a high density DNA array for an organism whose entire genome sequence is not available.
Kim, Eun Hye,Kim, Youn Shic,Park, Su-Hyun,Koo, Yeon Jong,Choi, Yang Do,Chung, Yong-Yoon,Lee, In-Jung,Kim, Ju-Kon American Society of Plant Physiologists 2009 Plant Physiology Vol.149 No.4
<P>Jasmonic acid (JA) is involved in plant development and the defense response. Transgenic overexpression of the Arabidopsis (Arabidopsis thaliana) jasmonic acid carboxyl methyltransferase gene (AtJMT) linked to the Ubi1 promoter increased levels of methyl jasmonate (MeJA) by 6-fold in young panicles. Grain yield was greatly reduced in Ubi1:AtJMT plants due to a lower numbers of spikelets and lower filling rates than were observed for nontransgenic (NT) controls. Ubi1:AtJMT plants had altered numbers of spikelet organs, including the lemma/palea, lodicule, anther, and pistil. The loss of grain yield and alteration in spikelet organ numbers were reproduced by treating NT plants with exogenous MeJA, indicating that increased levels of MeJA in Ubi1:AtJMT panicles inhibited spikelet development. Interestingly, MeJA levels were increased by 19-fold in young NT panicles upon exposure to drought conditions, resulting in a loss of grain yield that was similar to that observed in Ubi1:AtJMT plants. Levels of abscisic acid (ABA) were increased by 1.9- and 1.4-fold in Ubi1:AtJMT and drought-treated NT panicles, respectively. The ABA increase in Ubi1:AtJMT panicles grown in nondrought conditions suggests that MeJA, rather than drought stress, induces ABA biosynthesis under drought conditions. Using microarray and quantitative polymerase chain reaction analyses, we identified seven genes that were regulated in both Ubi1:AtJMT and drought-treated NT panicles. Two genes, OsJMT1 and OsSDR (for short-chain alcohol dehydrogenase), are involved in MeJA and ABA biosynthesis, respectively, in rice (Oryza sativa). Overall, our results suggest that plants produce MeJA during drought stress, which in turn stimulates the production of ABA, together leading to a loss of grain yield.</P>
Kim, Sang Gon,Kim, Sun Tae,Wang, Yiming,Kim, Sung-Kun,Lee, Chang Hoon,Kim, Keun-Ki,Kim, Ju-Kon,Lee, Sang Yeol,Kang, Kyu Young Blackwell Publishing Ltd 2010 Physiologia plantarum Vol.138 No.1
<P>Isoflavone reductase is an enzyme involved in isoflavonoid biosynthesis in plants. However, rice isoflavone reductase-like gene (<I>OsIRL,</I> accession no. AY071920) has not been unraveled so far. Here, we have characterized its behavior in response to oxidizing agents. Using Northern and Western blot analyses, the <I>OsIRL</I> gene and protein were shown to be down-regulated in young seedling roots treated with reduced glutathione (GSH) and diphenyleneiodonium (DPI), known quenchers of reactive oxygen species (ROS). The <I>OsIRL</I> transcript level in rice suspension-cultured cells was also found to be induced by oxidants such as hydrogen peroxide (H<SUB>2</SUB>O<SUB>2</SUB>), ferric chloride (FeCl<SUB>3</SUB>), methyl viologen (MV) and glucose/glucose oxidase (G/GO), but down-regulated when co-treated with GSH. Furthermore, to investigate whether overexpression of <I>OsIRL</I> in transgenic rice plants promotes resistance to ROS, we generated transgenic rice lines overexpressing the <I>OsIRL</I> gene under an abscisic acid (ABA) inducible promoter. Results showed that the <I>OsIRL</I> transgenic rice line activated by ABA treatment was tolerant against MV and G/GO-induced stress in rice leave and suspension-cultured cells. Our results strongly suggest the involvement of <I>OsIRL</I> in homeostasis of ROS.</P>
Kim, Hack-Youn,Kim, Kon-Joong,Lee, Jong-Wan,Kim, Gye-Woong,Choe, Ju-Hui,Kim, Hyun-Wook,Yoon, Yohan,Kim, Cheon-Jei Korean Society for Food Science of Animal Resource 2015 한국축산식품학회지 Vol.35 No.1
The aim of this study was to investigate the effects of various marination processes on the quality characteristics of chicken breast prepared with chicken feet gelatin and wheat fiber. The chicken feet gelatin was swollen with hydrochloric solution (0.1 N HCl, pH $1.31{\pm}0.02$) and dehydrated by freeze-drying. The composition (w/w) of the marinade was water (10%), soy sauce (12%), phosphate (0.3%), wheat fiber (1.5%), and chicken feet gelatin (1.5%). Three samples of chicken breast were manufactured with Tumbler (only tumbler), Tenderizer (tenderizer and tumbler), and Injector (injector and tumbler). The water content of the Injector sample was significantly higher than those of the Tumbler and Tenderizer samples (p<0.05). During heating, the lightness of all chicken breasts increased and the redness decreased. The tumbling and cooking yield of the Injector sample were significantly higher than those of the Tumbler and Tenderizer samples (p<0.05). The shear force of the Tenderizer sample was significantly lower than that of the Tumbler and Injector samples (p<0.05). No significant differences, except for color, were observed in the sensory analysis of the samples. Thus, the proper selection of mechanical processing is important to improve the quality characteristics of marinated chicken breast, considering the types of final products.