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- 저자 : Kazuko Yamaguchi Shinozaki (검색결과 4 건)
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Jin-Seok Moon,Satoshi Kidokoro,Daisuke Todaka,Sayuri Igusa,Junya Mizoi,Kazuo Shinozaki,Kazuko Yamaguchi-Shinozaki 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
As one of the most severe stress conditions, drought strongly affects the plant growth and productivity. OsPIL1, a gene encoding a rice Phytochrome Interacting Factor (PIF)-Like transcription factor, was found to be down-regulated under drought stress condition. OsPIL1 shows a diurnal expression pattern and known to be involved in regulation of plant height. However, the mechanisms of down-regulation of OsPIL1 expression under stress conditions are remained unclear. In this study, the expression of PIF4 and PIF5, the most homologous genes of OsPIL1 in Arabidopsis, was analyzed and the expression of these genes were found to be oscillated in circadian manner and down-regulated in response to drought and low temperature similar to that of OsPIL1. To identify the regions involved in the responses to drought, low temperature and diurnal cycle, the promoter analysis of PIF4 was performed using transgenic Arabidopsis. Further promoter analysis is ongoing to specify regulatory regions in more detail.
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식물배양세포의 저장관계 유전자탐색과 형질전환에의 활용 1. 벼 배양세포의 건조보존기구와 관련유전자 선발
김길웅,대야청춘,신동현,Virigool, S.,Shinzaki, K. Y.,홍석영 경북대학교 유전공학연구소 1995 遺傳工學硏究所報 Vol.10 No.1
A system for long-term dry preservation of rice (Oryza sativa L.) callus and a protection mechanism regulating the survival of dried callus were investigated. The highest survival of dried callus and the highest regeneration of plantlets were observed in calli which had been pretreated with 10^(-5) M abscisic acid (ABA) in the presence of 90 g/L of sucrose. A corresponding accumulation of the RNA of the rab 16A gene (a rice gene induced by ABA and water stress) was detected in dried callus, mature seeds, and callus pretreated with 10^(-5) M ABA. Analysis of protein by two dimensional polyacrylamide gel electrophoresis(2D-PAGE) demonstrated different protein patterns in dried callus pretreated with 10-s M ABA and 90 g/L of sucrose compared to callus dried without the pretreatment. Three different cDNA clones, pDHS1, pDHS2, and pDHS3 contained cDNA inserts in size of 3.8, 3.2, and 3.2 kilobase pairs, respectively, were isolated by using Nhe I fragment from rab 16A as a probe. All the three clones exhibited unique restriction maps.
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Genetic engineering approaches to understanding drought tolerance in plants
Zabta Khan Shinwari,Sohail Ahmad Jan,Kazuo Nakashima,Kazuko Yamaguchi-Shinozaki 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.2
Abiotic stresses such as drought, salinity, frost, etc., aff ect plant yield manyfold. These stresses can decrease the plant yield of important major crops up to 50%. The abiotic stress-related genes or other transcription factors (TFs) have multiple functions, as it increases proline content, leads closing of stomata to decrease the transpiration rate, enhances the production of some important stress-related protective enzymes, etc. and hence increases abiotic stress tolerance. Many TFs and other stressrelated genes have been identifi ed and characterized and transformed to many important cultivated plants against drought and others abiotic stresses. The transformed plants show better morpho-biochemical and physiological performances than non-transgenic plants. Many genetically engineered plants have been developed against drought stress including wheat, rice, tomato, soybean, cotton and many more. The effi ciently engineered clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated nuclease 9 (Cas9) system is now becoming a preferred choice of researchers to edit plant genomes for introgression natural resistance against a range of abiotic stresses. It leads genome editing by precise manure with minimal or no eff ect on growth and development of plants. Very limited reports are available to develop drought-tolerant plants using CRISPR/Cas9 system. Here we discuss transgenic plant technology and new [CRISPR Cas9 and Virus-Induced Gene Silencing (VIGS)] techniques to confer drought tolerance in important plant species.
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Sakuraba, Yasuhito,Kim, Eun-Young,Han, Su-Hyun,Piao, Weilan,An, Gynheung,Todaka, Daisuke,Yamaguchi-Shinozaki, Kazuko,Paek, Nam-Chon Oxford University Press 2017 Journal of experimental botany Vol.68 No.15
<▼1><P>In the tightly regulated chlorophyll biosynthesis pathway, the transcription factor Rice Phytochrome-Interacting Factor-Like1 promotes chlorophyll biosynthesis by up-regulating chlorophyll biosynthetic genes through feed-forward regulatory loops involving <I>GOLDEN2-LIKE1</I> (<I>OsGLK1</I>) and <I>OsGLK2</I>.</P></▼1><▼2><P><B>Abstract</B></P><P>In phototrophic plants, the highly conserved and tightly regulated process of chlorophyll (Chl) biosynthesis comprises multi-step reactions involving more than 15 enzymes. Since the efficiency of Chl biosynthesis strongly affects plant productivity, understanding the underlying regulatory mechanisms in crop plants can be useful for strategies to increase grain and biomass yields. Here, we show that rice (<I>Oryza sativa</I>) Phytochrome-Interacting Factor-Like1 (OsPIL1), a basic helix-loop-helix transcription factor, promotes Chl biosynthesis. The T-DNA insertion knockdown <I>ospil1</I> mutant showed a pale-green phenotype when grown in a natural paddy field. Transcriptome analysis revealed that several genes responsible for Chl biosynthesis and photosynthesis were significantly down-regulated in <I>ospil1</I> leaves. Using promoter binding and transactivation assays, we found that OsPIL1 binds to the promoters of two Chl biosynthetic genes, <I>OsPORB</I> and <I>OsCAO1</I>, and promotes their transcription. In addition, OsPIL1 directly up-regulates the expression of two transcription factor genes, <I>GOLDEN2-LIKE1</I> (<I>OsGLK1</I>) and <I>OsGLK2</I>. OsGLK1 and OsGLK2 both bind to the promoters of <I>OsPORB</I> and <I>OsCAO1</I>, as well as some of genes encoding the light-harvesting complex of photosystems, probably promoting their transcription. Thus, OsPIL1 is involved in the promotion of Chl biosynthesis by up-regulating the transcription of <I>OsPORB</I> and <I>OsCAO1</I> via trifurcate feed-forward regulatory loops involving two OsGLKs.</P></▼2>
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