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        Expression of antimicrobial peptide snakin‑1 confers efective protection in rice against sheath blight pathogen, Rhizoctonia solani

        Kaushik Das,Karabi Datta,Sailendra Nath Sarkar,Swapan Kumar Datta 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.1

        Rice sheath blight disease caused by the necrotrophic fungus Rhizoctonia solani, is an economically detrimental fungal disease which signifcantly afects rice productivity worldwide. Potato snakin-1, a cysteine-rich basic antimicrobial peptide (AMP), is a member of the novel Snakin AMP family. In order to assess the ability of the snakin-1 peptide in protecting rice against the sheath blight disease, we have developed transgenic rice constitutively expressing the snakin-1 peptide. The antimicrobial activity of snakin-1 was evaluated against the sheath blight pathogen Rhizoctonia solani both in vitro and in planta. Crude protein from transgenic rice leaves showed in vitro antifungal activity against Rhizoctonia solani. Moreover, in planta bioassay results also confrmed the same, wherein snakin-1 expressing rice plants showed signifcantly enhanced protection against the sheath blight disease. This report demonstrates how a member of the Snakin family of antimicrobial peptides has been successfully used to generate sheath blight resistance in rice, without compromising on its agronomic characteristics and at no phenotypic cost.

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        Analysis of molar flux and current density in the electrodialytic separation of sulfuric acid from spent liquor using an anion exchange membrane

        Beena Sheth,Kaushik Nath 한국화학공학회 2018 Korean Journal of Chemical Engineering Vol.35 No.9

        Separation of sulfuric acid from a dilute solution involved a plate and frame type electrodialysis unit using a commercial anion exchange membrane. Experiments were conducted in batch with catholyte concentrations ranging from 1 to 5 wt%. Effect of applied current density, initial catholyte concentration and initial concentration difference of catholyte and anolyte on the molar flux was studied extensively. The maximum molar flux was estimated to be 10.52×10-8 mol cm-2s-1 at 4.45 wt% catholyte concentration and applied current density of 30 mA cm2. Current efficiencies were observed to be 75 to 85% at lower current density, which rose to more than 100% at 20 and 30mA cm2, at equal initial concentration of catholyte and anolyte. Diffusive flux and flux due to membrane potential contributed very less compared to total flux in presence of applied electric current. An equation was developed to predict the practical molar fluxes, which fitted satisfactorily with minor standard deviation. Pristine and used membrane specimens were characterized using Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM).

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        Modeling of permeate flux and mass transfer resistances in the reclamation of molasses wastewater by a novel gas-sparged nanofiltration

        Tejal Manish Patel,Kaushik Nath 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.10

        A semi-empirical model has been applied to predict the permeate flux and mass transfer resistances duringthe cross flow nanofiltration of molasses wastewater in flat-sheet module. The model includes laminar flow regimeas well as flow in presence of gas sparging at two different gas velocities. Membrane hydraulic resistance (Rm), osmoticpressure resistance (Rosm) and the concentration polarization resistance (Rcp) were considered in series. The concentra-tion polarization resistance was correlated to the operating conditions, namely, the feed concentration, the trans-mem-brane pressure difference and the cross flow velocity for a selected range of experiments. There was an appreciablereduction of concentration polarization resistance Rcpsparin presence of gas sparging. Both the concentration polarizationresistance Rcplamand osmotic pressure resistance Rosm decreased with cross-flow velocity, but increased with feed con-centration and the operating pressure. Experimental and theoretical permeate flux values as a function of cross flowvelocity for both the cases, in the presence and absence of gas sparging, were also compared.

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