Alkaloids from Narcissus poeticus cv. Pink Parasol of various structural types and their biological activity

Marcela S ˇ afratova,Anna Hosˇt’a´lkova,Daniela Hulcova,Katerˇina Breiterova,Veronika Hrabcova,Marta Machado,Diana Fontinha,Miguel Prudeˆncio,Jirˇı´ Kunes,Jakub Chlebek,Daniel Jun,Martina Hrabinova,Lu 대한약학회 2018 Archives of Pharmacal Research Vol.41 No.2

Fifteen Amaryllidaceae alkaloids (1–15) of variousstructural types were isolated by standard chromatographicmethods from fresh bulbs of Narcissus poeticus cv. Pink Parasol. The chemical structures were elucidated byMS, and 1D and 2D NMR spectroscopic analyses, and bycomparison with literature data. Narcipavline (5) and narcikachnine(6) are reported here for the first time. In theirstructure are combined two basic structural types ofAmaryllidaceae alkaloids (galanthamine- and galanthindole-structural types), which represent a new structuraltype of these compounds. Alkaloids isolated in sufficientamounts were evaluated for their human erythrocyticacetylcholinesterase, and human serum butyrylcholinesterase(HuBuChE) inhibition activity using Ellman’smethod. Z-Gly-Pro-p-nitroanilide was used assubstrate in the prolyl oligopeptidase (POP) assay. Untestedalkaloids were also screened for their cytotoxic activityagainst a small panel of human cancer cells, which spannedcell lines from different tissue types. In parallel, MRC-5human fibroblasts were employed to determine overalltoxicity against noncancerous cells. Some compounds wereevaluated for their antiprotozoal activity. The newly isolatedalkaloid narcipavline (5) showed interestingHuBuChE inhibition activity (IC50 = 24.4 ± 1.2 lM), andnorlycoramine (11) demonstrated promising POP inhibition(IC50 = 0.21 ± 0.01 mM).

TRADITION AND CONTEXT: FRIENDS OR ENEMIES?

John L. Kater 한국민중신학회 2004 Madang: Journal of Contextual Theology Vol.2 No.-

THE RELIGIOUS DIMENSION OF AMERICAN IMPERIALISM

John L. Kater 성공회대학교 신학연구소 2008 Madang: Journal of Contextual Theology Vol.9 No.-

New Voices: Threat of Promise?

John L. Kater 한국민중신학회 2010 Madang: Journal of Contextual Theology Vol.0 No.14

This article uses examples drawn from contemporary Anglicanism to raise the question of the adequate hearing and reception of new voices within the established and mainstream traditions of Christianity. Citing the Indian theologian Christopher Duraisingh, it affirms the need to move beyond the traditional privilege accorded to English and English-speaking theologians,to accept that theology is always emerging from the encounter between tradition and context, and to ∞break open±the traditional institutions that stifled the diversity of voices within the churches. Listening to other voices casts new light on the Christian doctrines of salvation and incarnation as well as traditional approaches to ethics and worship. It also notes the importance of new voices for raising our awareness of the global and cosmic dimensions of Christian faith and for our relationship to other religious traditions. The article concludes with a challenge to those in positions of ecclesiastical and academic authority to pay special attention to voices which may contribute to their own deeper understanding of Christian faith.

The Religious Dimension of American Imperialism

John L.Kater 한국민중신학회 2008 Madang: Journal of Contextual Theology Vol.0 No.9

American imperialism is often considered to be similar to that practiced by other nations throughout history. This article argues that while it shares many characteristics with other forms of imperialism, an adequate understanding and response to American imperialism must take into account its unique religious interpretation of the nation’s destiny. This facet of American national identity is ultimately derived from the faith of the early Puritan and Separatist settlers of New England who believed that their history reflected the history of the people of Israel. It lent itself to a “civil religion” that continued to conceive of the United States as a “light to the nations” with a special God-given mission and calling, and has recently re-emerged in militant American Evangelicalism.

TRADITION AND CONTEXT: FRIENDS OR ENEMIES?

John L,Kater 성공회대학교 신학연구소 2004 Madang: Journal of Contextual Theology Vol.2 No.-

Effect of Calpain Inhibitors on $Ca^{2+}-Induced$ Suppression of Neurite Outgrowth in Isolated Hippocampal Pyramidal Neurons

Song, Dong-Keun,Kater, Stanley B.,Mykles, Donald L. The Korean Society of Pharmacology 1993 대한약리학잡지 Vol.29 No.2

칼슘이온은 신경섬유 성장의 중요한 조절인자이나 그 정확한 작용기전은 불명확하다. 세포골격 단백은 in vivo 및 in vitro에서 칼슘의존성 단백분해효소(칼파인)에 의해 신속히 분해되므로, 칼슘이온에 의한 신경섬유의 퇴행에 있어서 칼파인의 관련성을 추구하기위하여, 배양된 해마신경세포에서 칼슘이온 ionophore인 A23187에 의한 신경섬유의 성장억제가 칼파인의 억제제인 EST 및 MDL 28170에 의해 차단되는지를 조사하였다. A23187은 100nM의 농도에서 축삭에는 영향이 없이 수상돌기의 퇴행을 유발하였으나, 300 nM의 농도에서는 축삭의 성장을 억제하였다. EST(5 혹은 20 uM) 및 MDL 28170(20 uM)은 100 nM A23187의 수상돌기에 대한 작용과 300 nM A23187의 축삭에 대한 작용을 효과적으로 차단하였다. EST는 A23187에의한 세포내 칼슘이온의 증가를 차단하지 못하였다. 이상의 결과는 해마추상체세포에서 칼슘에 의한 신경섬유의 퇴행이 칼파인에 의해 매개됨을 시사한다. $Ca^{2+}$ is an important regulator of neurite elongation and growth cone movements but the mechanism(s) mediating these $Ca^{2+}-dependent$ effects is unclear. Since cytoskeletal proteins are rapidly degraded by $Ca^{2+}-dependent $ proteinases (calpains) in vitro and in vivo, we investigated whether $Ca^{2+}-induced$ pruning or regression of neuronal processes is mediated by calpains. Isolated hippocampal pyramidal-like neurons were cultured and the ability of the membrane-permeable calpain inhibitors EST (etyl (+)-(2S,3S)-3-[(S)-methyl-1-(3-methlbutylcarbamoyl)-butylcarbamoyl]2-oxiranecarboxylate) and MDL28170 (carbobenzoxyl-Val-Phe-H) to block the $Ca^{2+}$ ionophore A23187-induced suppression in neurite outgrowth was investigated. Addition of 100 nM A23187 to the culture medium resulted in a retraction of dendrites without altering axonal elongation. The addition of 300 nM A23187 to the culture medium resulted in a signiciant decrease in the rate of axonal elongation as well as a retraction of dendritic processes. Administration of EST $(5\;or\;20{\mu}M)$ to the culture medium completely blocked the pruning effect of 100 nM A23187 on dendrites and of 300 nM A23187 on axons, while EST alone did not significantly affect neurite outgrowth rate. MDL 28170 $(20\;{\mu}M)$ showed the same effect as EST in preventing ionophore-induced pruning of dendrites and axons at 100 nM and 300 nM concentrations, respectively, of A23187. EST $(20\;{\mu}M)$ did not block the A23187-induced rise of $[Ca^{2+}]_{i}$ as measured with fura-2. These results show that $Ca^{2+}-induced$ pruning of neurites in isolated hippocampal pyramidal neurons is mediated by calpains.

해마추상체 신경세포에서 칼슘에 의한 신경섬유 성장억제에 대한 칼파인 억제제의 영향

송동근(Dong-Keun Song),Stanley B. Kater,Donald L. Mykles 대한약리학회 1993 대한약리학잡지 Vol.29 No.2

칼슘이온은 신경섬유 성장의 중요한 조절인자이나 그 정확한 작용기전은 불명확하다. 세포골격 단백은 in vivo 및 in vitro에서 칼슘의존성 단백분해효소(칼파인)에 의해 신속히 분해되므로, 칼슘이온에 의한 신경섬유의 퇴행에 있어서 칼파인의 관련성을 추구하기위하여, 배양된 해마신경세포에서 칼슘이온 ionophore인 A23187에 의한 신경섬유의 성장억제가 칼파인의 억제제인 EST 및 MDL 28170에 의해 차단되는지를 조사하였다. A23187은 100nM의 농도에서 축삭에는 영향이 없이 수상돌기의 퇴행을 유발하였으나, 300 nM의 농도에서는 축삭의 성장을 억제하였다. EST(5 혹은 20 uM) 및 MDL 28170(20 uM)은 100 nM A23187의 수상돌기에 대한 작용과 300 nM A23187의 축삭에 대한 작용을 효과적으로 차단하였다. EST는 A23187에의한 세포내 칼슘이온의 증가를 차단하지 못하였다. 이상의 결과는 해마추상체세포에서 칼슘에 의한 신경섬유의 퇴행이 칼파인에 의해 매개됨을 시사한다. Ca²⁺ is an important regulator of neurite elongation and growth cone movements but the mechanism(s) mediating these Ca²⁺-dependent effects is unclear. Since cytoskeletal proteins are rapidly degraded by Ca²⁺-dependent proteinases (calpains) in vitro and in vivo, we investigated whether Ca²⁺-induced pruning or regression of neuronal processes is mediated by calpains. Isolated hippocampal pyramidal-like neurons were cultured and the ability of the membrane-permeable calpain inhibitors EST (etyl (+)-(2S,3S)-3-[(S)-methyl-1-(3-methlbutylcarbamoyl)-butylcarbamoyl]2-oxiranecarboxylate) and MDL28170 (carbobenzoxyl-Val-Phe-H) to block the Ca²⁺ ionophore A23187-induced suppression in neurite outgrowth was investigated. Addition of 100 nM A23187 to the culture medium resulted in a retraction of dendrites without altering axonal elongation. The addition of 300 nM A23187 to the culture medium resulted in a signiciant decrease in the rate of axonal elongation as well as a retraction of dendritic processes. Administration of EST (5 or 20μM) to the culture medium completely blocked the pruning effect of 100 nM A23187 on dendrites and of 300 nM A23187 on axons, while EST alone did not significantly affect neurite outgrowth rate. MDL 28170 (20μM) showed the same effect as EST in preventing ionophore-induced pruning of dendrites and axons at 100 nM and 300 nM concentrations, respectively, of A23187. EST (20μM) did not block the A23187-induced rise of [Ca²⁺]_i as measured with fura-2. These results show that Ca²⁺-induced pruning of neurites in isolated hippocampal pyramidal neurons is mediated by calpains.

The D-lineage MADS-box gene <i>OsMADS13</i> controls ovule identity in rice

Dreni, Ludovico,Jacchia, Sara,Fornara, Fabio,Fornari, Monica,Ouwerkerk, Pieter B. F.,An, Gynheung,Colombo, Lucia,Kater, Martin M. Blackwell Publishing Ltd 2007 The Plant journal Vol.52 No.4

<P>Summary</P><P>Genes that control ovule identity were first identified in <I>Petunia</I>. Co-suppression of both <I>FLORAL BINDING PROTEIN 7</I> (<I>FBP7</I>) and <I>FBP11</I>, two D-lineage genes, resulted in the homeotic transformation of ovules into carpelloid structures. Later in Arabidopsis it was shown that three genes, <I>SHATTERPROOF1</I> (<I>SHP1</I>), <I>SHP2,</I> and <I>SEEDSTICK</I> (<I>STK</I>), redundantly control ovule identity, because in the <I>stk shp1 shp2</I> triple mutant ovules lose identity and are transformed into carpel and leaf-like structures. Of these three Arabidopsis genes <I>STK</I> is the only D-lineage gene, and its expression, like <I>FBP7</I> and <I>FBP11</I>, is restricted to ovules. <I>OsMADS13</I> is the rice ortholog of <I>STK</I>, <I>FBP7,</I> and <I>FBP11</I>. Its amino acid sequence is similar to the Arabidopsis and <I>Petunia</I> proteins, and its expression is also restricted to ovules. We show that the <I>osmads13</I> mutant is female sterile and that ovules are converted into carpelloid structures. Furthermore, making carpels inside carpels, the <I>osmads13</I> flower is indeterminate, showing that <I>OsMADS13</I> also has a function in floral meristem determinacy. <I>OsMADS21</I> is most likely to be a paralog of <I>OsMADS13</I>, although its expression is not restricted to ovules. Interestingly, the <I>osmads21</I> mutant did not show any obvious phenotype. Furthermore, combining the <I>osmads13</I> and the <I>osmads21</I> mutants did not result in any additive ovule defect, indicating that <I>osmads21</I> does not control ovule identity. These results suggest that during evolution the D-lineage gene <I>OsMADS21</I> has lost its ability to determine ovule identity.</P>

심방세동의 수술적 치료

Cox, James L.,Boineau, John P.,Schuessler, Richard B.,Ferguson, T. Bruce,Cain, Michael E.,Lindsay, Bruce D.,Corr, Peter B.,Kater, Kathy M.,Lappas, Demeterios G.,장낭수 대한의학협회 1992 대한의학협회지 Vol.35 No.5