http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Kanoi, Bernard N. (검색결과 1 건)
- 무료
- 기관 내 무료
- 유료
-
Sakamoto, Hirokazu,Takeo, Satoru,Takashima, Eizo,Miura, Kazutoyo,Kanoi, Bernard N.,Kaneko, Takamasa,Han, Eun-Taek,Tachibana, Mayumi,Matsuoka, Kazuhiro,Sattabongkot, Jetsumon,Udomsangpetch, Rachanee,Is Elsevier 2018 Parasitology international Vol.67 No.2
<P><B>Abstract</B></P> <P>The target molecules of antibodies against falciparum malaria remain largely unknown. Recently we have identified multiple proteins as targets of immunity against <I>Plasmodium falciparum</I> using African serum samples. To investigate whether potential targets of clinical immunity differ with transmission intensity, we assessed immune responses in residents of low malaria transmission region in Thailand. Malaria asymptomatic volunteers (Asy: n=19) and symptomatic patients (Sym: n=21) were enrolled into the study. Serum immunoreactivity to 186 wheat germ cell-free system (WGCFS)-synthesized recombinant <I>P. falciparum</I> asexual-blood stage proteins were determined by AlphaScreen, and subsequently compared between the study groups. Forty proteins were determined as immunoreactive with antibody responses to 35 proteins being higher in Asy group than in Sym group. Among the 35 proteins, antibodies to MSP3, MSPDBL1, RH2b, and MSP7 were significantly higher in Asy than Sym (unadjusted p<0.005) suggesting these antigens may have a protective role in clinical malaria. MSP3 reactivity remained significantly different between Asy and Sym groups even after multiple comparison adjustments (adjusted p=0.033). Interestingly, while our two preceding studies using African sera were conducted differently (e.g., cross-sectional vs. longitudinal design, observed clinical manifestation vs. functional activity), those studies similarly identified MSP3 and MSPDBL1 as potential targets of protective immunity. This study further provides a strong rationale for the application of WGCFS-based immunoprofiling to malaria vaccine candidate and biomarker discovery even in low or reduced malaria transmission settings.</P> <P><B>Highlights</B></P> <P> <UL> <LI> WGCFS generated <I>P. falciparum</I> recombinant proteins are immunoreactive to human sera from low endemic Thailand. </LI> <LI> Four <I>P. falciparum</I> antigens are plausible targets of clinical immunity. </LI> <LI> WGCFS and AlphaScreen system are invaluable tools for malaria vaccine candidate and biomarker discovery. </LI> </UL> </P>
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
