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Jwalant K. Bhatt,Chirag M. Ghevariya,Dushyant R. Dudhagara,Rahul K. Rajpara,Bharti P. Dave 한국미생물학회 2014 The journal of microbiology Vol.52 No.11
For the first time, Cochliobolus lunatus strain CHR4D, amarine-derived ascomycete fungus isolated from historicallycontaminated crude oil polluted shoreline of Alang-Sosiyaship-breaking yard, at Bhavnagar coast, Gujarat has beenreported showing the rapid and enhanced biodegradationof chrysene, a four ringed high molecular weight (HMW)polycyclic aromatic hydrocarbon (PAH). Mineral Salt Broth(MSB) components such as ammonium tartrate and glucosealong with chrysene, pH and trace metal solution have beensuccessfully optimized by Response Surface Methodology(RSM) using central composite design (CCD). A validated,two-step optimization protocol has yielded a substantial93.10% chrysene degradation on the 4th day, against unoptimized56.37% degradation on the 14th day. The resultsdepict 1.65 fold increase in chrysene degradation and 1.40fold increase in biomass with a considerable decrement intime. Based on the successful laboratory experiments, C. lunatusstrain CHR4D can thus be predicted as a potentialcandidate for mycoremediation of HMW PAHs impactedenvironments.