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      • KCI등재후보

        Mutation breeding for determinate, diseases, lodging resistance and high yield in sesame

        Chul Whan Kang(姜哲煥),Jung Il Lee(李正日),Rae Kyeong Park(朴來敬),Byung Han Choi(崔炳漢) 한국육종학회 1993 한국육종학회지 Vol.25 No.3

        Fourteen sesame varieties have been released to farmer’s field since 1955 in Korea. Two varieties were bred by mutation breeding method out of the varieties, and X-ray 20 Krad-irradiated “Ahnsankkae” was developed in 1984 and has been being major sesame variety in Korea. It is occupied 31% of total sesame area due to higher yield uniformity in different regions and diseases resistance. Suwonkkae” was newly developed in 1991 from a X-ray irradiated mutant crossed to Korean local variety and had superior quality with higher essential amino acids and linoleic(Vitamin F) fatty acid content. Mutant 2,625 pedigree lines and 89,200 mutants of all generations were planted to the experimental field of Crop Experiment Station to screen and investigate diseases resistance, yielding ability and the other usable characteristics from 1989 to 1992 for sesame mutation breeding programme in Korea. 1, 540-mutant lines and 2,137g of M1 seeds were selected among all the released mutant lines and plants of all the generations from 1989 to 1992. Sodium azide(NaN3)-treated dwarf mutant “Suwon 128” had been promising due to it’s unique shape of dwarf with strong lodging resistance and higher yield possibility when planted densely. However, it was deleted from presenting recommending varieties in 1992 by the reason of weakness to phytophthora blight specially at the full maturing stage of harvesting time during the 3 years regional yield trials(RYT) in spite of similar yield performance compared to the recommended varieties. The only one determinate gene in the world “dt 45” irradiated 50 Krad γ-ray by Dr. A. Ashri has been promising gene in Korea for higher maturity rate and synchronization of flowering period to improve longer flowering period(40-50 days) of the indeterminate varieties. “dt 45” was beginning to be introduced to cross breeding programme from 1985, and “Suwon 129” was bred from the cross between “dt 45” and “Danbaeckkae”, and was released to regional yield trial from 1990 for the first time as a determinate type line in Korea. Determinate lines Suwon 131, 133, 134, and 135 were continuously released to regional yield trial following after Suwon 129, but almost all the lines of determinate showed lesser yield performance due to their few capsule setting nodes(3-5) on the stem than recommended indeterminate varieties in spite of their strong resistance to diseases and lodging and higher maturity rate. 5,015 lines(57%) and 1,229(58%) cross combinations of mutants and mutant crosses among 8,751 lines and 2,122 cross combinations of whole cross breeding pedigree nurseries have been planted and evaluated since 1989. 1,266 lines(60%) and 276(54%) cross combinations of mutants and mutant crosses among 2,096 lines and 509 cross combinations of whole yield performance trials(OYT, PYT, AYT and RYT) have been relaeased since 1989 in Korea. Consequently dwarf mutant “Suwon 128” and determinate varieties have been composed of one hundred percent in the crossing block in 1992. Determinate, semi-dwarf, diseases and lodging resistant, larger seed size, few and non branches along with higher maturity rate and yield have been illustrated as Ideal Plant Type of sesame breeding target in Korea. “SI 90033-2B” (Suwon 129//Suwon 9/IS 103) mutant which was found in F₂ pedigree nursery in 1992 showed strong resistance to diseases and lodging, plenty of capsule-setting nodes with longer capsule bearing position, lots of capsules and higher maturity rate compared to those of indeterminate and of other determinate mutants in Korea. It has been evaluated to overcome yield problem of determinates due to idealistic growth and yield components. “SI 90033-2B” has been believed to be the mutant line for creating Ideal Plant Type in sesame.

      • KCI등재후보

        Thin-layer chromatographic separations of unsaponifiable components in seed oil of wild and cultivated sesame

        Su Noh Ryu(柳守魯),Jung Il Lee(李正日),Chul Whan Kang(姜哲煥) 한국육종학회 1995 한국육종학회지 Vol.27 No.3

        This study was conducted to obtain basic informations on the unsaponifiable components in sesame seed. Four major lignan, sesamin, sesamolin, sesamol and sesangolin were analyzed to provide fine separation of unsaponifiable component fractions of seed oil in four wild and three cultivated sesame varieties by thin layer chromatography (TLC). The mean content of unsaponifiable components extracted from cultivated sesame oil was 2.4% (ranged 2.2 to 2.7), while that of wild ones showed 3.3% (ranged 2.3 to 4.0). The mean content of unsaponifiable components of wild species was higher than that of cultivated ones. Silicagel plates and chloroform/diethyl ether (9 : 1, v/v) as mobile phase on the first and second directions were utilized in TLC analysis. In one-dimensional TLC separation, sesamin, sesamolin, sesamol, sesangolin and tocopherol were identified as spots at the Rf values of 0.66, 0.76, 0.52, 0.66 and 0.88, respecitively. Sesamin and sesangolin were appeared at same spots with Rf value of 0.66. In two-dimensional TLC separation, sesamin and sesangolin showed different TLC pattern. This two-dimensional system could be adopted to provide pure fractions in preparative experiments for sesamin and sesangolin measurements of seed oil. Some other minor spots were also observed in S. alatum and S. radiatum showing void of sesamolin but containing sesangolin.

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