Molecular analysis of T-DNA insertion mutant population for transgenic rice plants

Hyemin Lim,A-Ram Kim,Jung-Il Cho,Hyeon-So Ji,Ung-Han Yoon,Tae-Ho Kim,Gang-Seob Lee 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

We have generated 383 independent transgenic lines for genetically modified (GM) rice that contained GPD, UtrCSP, BrTSR15 and BrTSR53 genes overexpression constructs under the control of the constitutive CaMV 35S promoter. TaqMan copy number assay was determined inserted T-DNA copy number. Also FSTs analysis was isolated from 203 single copy T-DNA lines of transgenic plants and sequence mapped to the rice chromosomes. In analyzing single copy lines, we identified 95 FSTs, among which 37 (38.9%) were integrated into genic regions and 58 (61.1%) into intergenic regions. About 27 homozygous lines were obtained through multi-generations of planting, resistance screening and TaqMan copy number assay. To investigate the transgene expression patterns, quantitative real-time PCR analysis was performed using total RNAs from leaf tissue of single copy, intergenic region of T-DNA insertion and homozygous T2 plants. The mRNA expression levels of the examined transgenic rice were significantly increased in all of the transgenic plants. In addition, myc-tagged 35S::BrTSR15 and 35S::BrTSR53 transgenic plants were displayed higher levels of transgene protein than WT plants. These results may be useful for producing of large-scale transgenic plants or T-DNA inserted mutants in rice.

Generation and characterization of T-DNA insertion population for genetically-modified rice

Hyemin Lim,A-Ram Kim,Hyun-Ju Hwang,Jung-Il Cho,Hyeonso Ji,Chang-Kug Kim,Soo-Chul Park,Gang-Seob Lee 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

We have generated 383 independent transgenic lines for genetically modified (GM) rice that contained PsGPD (Glyceraldehyde-3-Phosphate Dehydrogenase), ArCspA (Cold Shock Protein), BrTSR15 (Triple Stress Resistance 15) and BrTSR53 (Triple Stress Resistance 53) genes over-expression constructs under the control of the constitutive (CaMV 35S) promoter. TaqMan copy number assay was determined inserted T-DNA copy number. Also flanking sequence tags (FSTs) analysis was isolated from 203 single copy T-DNA lines of transgenic plants and sequence mapped to the rice chromosomes. In analyzing single copy lines, we identified 157 flanking sequence tags (FSTs), among which 58 (36%) were integrated into genic regions and 97 (62%) into intergenic regions. About 27 putative homozygous lines were obtained through multi-generations of planting, resistance screening and TaqMan copy number assay. To investigate the transgene expression patterns, quantitative real-time PCR analysis was performed using total RNAs from leaf tissue of single copy, intergenic region of T-DNA insertion and homozygous T2 plants. The mRNA expression levels of the examined transgenic rice were significantly increased in all of the transgenic plants. In addition, myc-tagged 35S:BrTSR15 and 35S:BrTSR53 transgenic plants were displayed higher levels of transgene protein. These results may be useful for producing of large-scale transgenic plants or T-DNA inserted mutants in rice.

Zinc finger protein 746 promotes colorectal cancer progression via c-Myc stability mediated by glycogen synthase kinase 3β and F-box and WD repeat domain-containing 7

Jung, Ji Hoon,Jung, Deok-Beom,Kim, Hyunseok,Lee, Hyemin,Kang, Shi-Eun,Srivastava, Sanjay K.,Yun, Miyong,Kim, Sung-Hoon Nature Publishing Group UK 2018 Oncogene Vol.37 No.27

<P>To elucidate the underlying oncogenic mechanism of zinc finger protein 746 (ZNF746), current study was conducted in colorectal cancers (CRCs). Herein, ZNF746 was overexpressed in HCT116, SW620, and SW480 cells, which was supported by CRC tissue microarray and TCGA analysis. Also, DNA microarray revealed the differentially expressed gene profile particularly related to cell cycle genes and c-Myc in ZNF746 depleted HCT116 cells. Furthermore, ZNF746 enhanced the stability of c-Myc via their direct binding through nuclear colocalization by immunoprecipitation and immunofluorescence, while ZNF746 and c-Myc exist mainly in nucleoplasm. Conversely, ZNF746 depletion attenuated phosphorylation of c-Myc (S62) and glycogen synthase kinase 3 beta (GSK3 beta) (S9) and also activated p-c-Myc (T58), which was reversed by GSK3 inhibitors such as SB-216763 and Enza. Also, c-Myc degradation by ZNF746 depletion was blocked by knockdown of F-box/WD repeat-containing protein 7 (FBW7) ubiquitin ligase or proteosomal inhibitor MG132. Additionally, the growth of ZNF746 depleted HCT116 cancer cells was retarded with decreased expression of ZNF746 and c-Myc. Overall, these findings suggest that ZNF746 promotes CRC progression via c-Myc stability mediated by GSK3 and FBW7.</P>

The Impact of an Emergency Fee Increase on the Composition of Patients Visiting Emergency Departments

Jung, Hyemin,Do, Young Kyung,Kim, Yoon,Ro, Junsoo The Korean Society for Preventive Medicine 2014 Journal of Preventive Medicine and Public Health Vol.47 No.6

Objectives: This study aimed to test our hypothesis that a raise in the emergency fee implemented on March 1, 2013 has increased the proportion of patients with emergent symptoms by discouraging non-urgent emergency department visits. Methods: We conducted an analysis of 728 736 patients registered in the National Emergency Department Information System who visited level 1 and level 2 emergency medical institutes in the two-month time period from February 1, 2013, one month before the raise in the emergency fee, to March 31, 2013, one month after the raise. A difference-in-difference method was used to estimate the net effects of a raise in the emergency fee on the probability that an emergency visit is for urgent conditions. Results: The percentage of emergency department visits in urgent or equivalent patients increased by 2.4% points, from 74.2% before to 76.6% after the policy implementation. In a group of patients transferred using public transport or ambulance, who were assumed to be least conscious of cost, the change in the proportion of urgent patients was not statistically significant. On the other hand, the probability that a group of patients directly presenting to the emergency department by private transport, assumed to be most conscious of cost, showed a 2.4% point increase in urgent conditions (p<0.001). This trend appeared to be consistent across the level 1 and level 2 emergency medical institutes. Conclusions: A raise in the emergency fee implemented on March 1, 2013 increased the proportion of urgent patients in the total emergency visits by reducing emergency department visits by non-urgent patients.

A derivative of epigallocatechin-3-gallate induces apoptosis via SHP-1-mediated suppression of BCR-ABL and STAT3 signalling in chronic myelogenous leukaemia : EGCG-MP effectively induces apoptosis in CML

Jung, Ji Hoon,Yun, Miyong,Choo, Eun-Jeong,Kim, Sun-Hee,Jeong, Myoung-Seok,Jung, Deok-Beom,Lee, Hyemin,Kim, Eun-Ok,Kato, Nobuo,Kim, Bonglee,Srivastava, Sanjay K,Kaihatsu, Kunihiro,Kim, Sung-Hoon Wiley (Blackwell Publishing) 2015 British journal of pharmacology Vol.172 No.14

Mortality After Acute Coronary Syndrome in Human Immunodeficiency Virus Infection with Optimal Adherence: A Nationwide Study

Jung Hyemin,Lee Eunyoung,Ro Jun-soo,Lee Jin Yong,Bang Ji Hwan 대한감염학회 2023 Infection and Chemotherapy Vol.55 No.4

Background: There have been few studies on the outcome of acute coronary syndrome (ACS) in human immunodeficiency virus (HIV) infection in the era when antiretroviral therapy (ART) is generalized and most of them have achieved viral suppression. Using claims data, we aimed to assess the mortality after atherosclerotic cardiovascular events in people with HIV (PWH) who maintain optimal adherence to ART. Materials and Methods: We used claims data from the National Health Insurance of the Korea to confirm newly diagnosed PWH from 2009 to 2019, and measured ART adherence. ACS and mortality were confirmed in PWH who showed optimal adherence to ART. Results: Among 7,100 PWH with optimal adherence and during 27,387 person-year of follow-up duration, ACS was confirmed in 140 (2.0%) cases, which was 1.3 times greater than statistics of the Korean general population (511.0 vs. 383.1 per 100,000). Acquired immunodeficiency syndrome, hypertension, dyslipidemia, and diabetes mellitus were associated with the development of ACS in PWH with optimal adherence. Mortality was confirmed in 10 cases, which is 7.1% overall and 2.9% when limited to myocardial infarction. It was comparable with the mortality rate of the Korean general population after myocardial infarction (8.9%). Conclusion: ACS prevalence was higher in PWH even when optimal adherence was maintained. However, mortality after ACS was comparable to that in the HIV-negative population.

Prevalence of antineuronal antibodies in patients with encephalopathy of unknown etiology: Data from a nationwide registry in Korea

Byun, Jung-Ick,Lee, Soon-Tae,Jung, Keun-Hwa,Sunwoo, Jun-Sang,Moon, Jangsup,Kim, Tae-Joon,Lim, Jung-Ah,Kim, Soyun,Kim, Do-Yong,Han, Su-Hyun,Jang, Hyemin,Suh, Hong Il,Cho, A-Hyun,Kim, Dong Wook,Shin, Ju Elsevier 2016 Journal of neuroimmunology Vol.293 No.-

<P><B>Abstract</B></P> <P>We aimed to evaluate the prevalence of antineuronal antibodies in a nationwide cohort of patients with encephalopathy of unknown etiology. We screened 1699 patients with idiopathic encephalopathy who were referred from 70 hospitals across Korea for autoimmune synaptic and classic paraneoplastic antibodies. Those with cerebellar degeneration, sensory polyneuropathy or other paraneoplastic syndromes without encephalopathy were not included in this study. One-hundred and four patients (6.12%) had antibody-associated autoimmune encephalopathy. Autoimmune synaptic antibodies were identified in 89 patients (5.24%) and classic paraneoplastic antibodies were identified in 16 patients (0.94%). The patients with antibody-associated autoimmune encephalopathy comprised a small but significant portion of the total number of patients with encephalopathy of unknown cause.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Among 1699 patients with encephalopathy of unknown etiology, autoantibody was identified in 6.12%. </LI> <LI> Autoimmune encephalopathy comprised a significant portion of idiopathic cases. </LI> <LI> Anti-NMDA receptor antibody was the most common autoantibody in age under 40. </LI> <LI> Anti-LGI1 antibody was the most common autoantibody in age 40 and over. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

MS-전자코를 사용한 페트(polyethylene terephthalate) 생수병과 뚜껑의 이취 이행 분석

한현정(Hyun Jung Han),박수원(Su Won Park),정효연(Hyo Yeon Jung),김정선(Jung Sun Kim),동혜민(Hyemin Dong),노봉수(Bong Soo Noh) 한국식품과학회 2015 한국식품과학회지 Vol.47 No.4

원료인 생수와 포장 용기인 페트병과 뚜껑에서 야기되는 이취성분의 존재여부를 MS-전자코를 통하여 분석해 보았다. 이취가 나는 생수는 농도별로 DF2 양의 방향으로 이동하는 것을 확인할 수 있었고, 이취의 원인이 페트병 혹은 페트병의 뚜껑이라고 가정하여 분석을 진행한 결과 뚜껑에서 휘발 성분이 페트병 보다 많이 검출되는 것으로 나타났다. 이는 물에서 나는 이취가 페트병보다는 뚜껑에 영향을 더 많이 받았을 것이라고 볼 수 있었다. 또한 이온 분획의 결과로 보아 이취의 원인이 되는 물질인 2,4-di-tert-butylphenol, 노난올과 데칸올이 페트병과 뚜껑에 포함되어 있다고 추측되었다. The objective of the study was to investigate the off-flavor generated from PET water bottles and their caps by using a mass spectrometry-based electronic nose. The ion fragment data obtained from the electronic nose were used for discriminant function analysis (DFA). In the case of increased concentrations of the contamination of water, the off-flavor pattern depended on the discriminant function second score instead of the discriminant function first score. To identify the cause of off-flavor in polyethylene terephthalate (PET) bottled water, the PET bottle and its cap were analyzed by DFA. The results showed that the cap generated more volatile compounds than the bottle or mineral water did. The substances causing the off-flavor were predicted to be 2,4-di-tert-butylphenol (2,4-DTBP), nonanal, and decanal when the main peak of the mass spectrum was compared with the major ion fragments of the electronic nose. Thus, using this method, we could determine whether the PET water bottle was contaminated and whether the off-flavor resulted from contamination of the bottle cap.

CNOT2 promotes proliferation and angiogenesis via VEGF signaling in MDA-MB-231 breast cancer cells

Sohn, Eun Jung,Jung, Deok-Beom,Lee, HyoJung,Han, Ihn,Lee, Jihyun,Lee, Hyemin,Kim, Sung-Hoon Elsevier 2018 Cancer letters Vol.412 No.-

<P><B>Abstract</B></P> <P>Here the underlying role of CNOT2, a subunit of CCR4-NOT complex, was elucidated in cancer progression. CNOT2 was overexpressed in HIT-T15, ASPC-1, BXPC-3, PC-3, LNCaP, MCF-7 and MDA-MB-231 cell lines, which was confirmed by Tissue array in various human tumor tissues. Also, CNOT2 depletion suppressed proliferation and colony formation of MDA-MB-231 cells. Of note, microarray revealed decreased expression of CNOT2, VEGF-A, HIF2 alpha (<0.5 fold) and increased expression of UMOD1, LOC727847, MMP4, hCG and other genes (>2.0 fold) in CNOT2 depleted MDA-MB-231 cells compared to untreated control. Consistently, downregulation of VEGF, CNOT2 and HIF2 alpha was verified in CNOT2 depleted MDA-MB-231 cells by RT-qPCR. Additionally, CNOT2 depletion inhibited VEGF induced tube formation in HUVECs and reduced neovascularization in CAM assay. Furthermore, the growth of CNOT2 depleted MDA-MB-231 cells was significantly reduced in Balb/c nude mice along with decreased expression of VEGF and PCNA by immunohistochemistry compared to untreated control group. Overall, our findings provide evidences that CNOT2 promotes proliferation and angiogenesis via VEGF signaling in MDA-MB-231 breast cancer cells as a potent molecular target for breast cancer treatment.</P> <P><B>Highlights</B></P> <P> <UL> <LI> CNOT2 was overexpressed in HIT-T15, ASPC-1, BXPC-3, PC-3, LNCaP, MCF-7 and MDA-MB-231 cell lines compared to normal cells. </LI> <LI> CNOT2 depletion suppressed proliferation and colony formation of MDA-MB-231 cells. </LI> <LI> CNOT2 depletion inhibited VEGF induced tube formation in HUVECs and reduced neovascularization in CAM assay. </LI> <LI> CNOT2 depletion suppressed the growth of MDA-MB-231 cells in Balb/c nude mice along with decreased expression of VEGF and PCNA by IHC. </LI> <LI> Overall, CNOT2 promotes proliferation and angiogenesis via VEGF signaling in MDA-MB-231 breast cancer cells. </LI> </UL> </P>

Over-expression of PsGPD, a mushroom glyceraldehyde-3-phosphat dehydrogenase gene, enhances salt tolerance in rice plants

Dan-Be Park,Jung-Il Cho,Hyemin Lim,Zamin Shaheed Siddiqui,Sung-Han Park,Mi-Jeong Jeong,Gang-Seob Lee,Soo-Chul Park 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

Transgenic potatoes expressing glyceraldehyde-3-phosphate dehydrogenase (GPD), isolated from the oyster mushroom, Pleurotus sajor-caju, had increased tolerance to salt stress (Jeong et al. 2001). To examine the physiological mechanisms enhancing salt tolerance in GPD transgenic rice plants, the salt tolerance of five GPD transgenic rice lines (T1–T5) derived from Dongjin rice cultivar was tested in a fixed 150-mM saline environment in comparison to two known wild-type rice cultivars, Dongjin (salt sensitive) and Pokali (salt tolerant). Transgenic lines T2, T3, and T5 showed a substantial increase in biomass and relative water content compared to Dongjin. Stomatal conductance and osmotic potential were higher in the GPD transgenic lines and were similar to those in Pokali. The results are discussed based on the comparative physiological response of GPD transgenic lines with those of the salt-sensitive and salt-tolerant rice cultivars.