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Jung, B. K.,Khan, A. R.,Hong, S. J.,Park, G. S.,Park, Y. J.,Kim, H. J.,Jeon, H. J.,Khan, M. A.,Waqas, M.,Lee, I. J. UNIV. OF MILAN DEPARTMENT OF FOOD SCIENCE AND MICR 2017 ANNALS OF MICROBIOLOGY Vol.67 No.9
<P>Plant growth-promoting rhizobacteria (PGPR) affect plant growth through various mechanisms, such as indole-3-acetic acid (IAA) production, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, and biofilm formation. The aim of the study reported here was to isolate and characterize rhizobacteria that produce quorum-sensing signal molecules and other PGPR-related molecules. A biofilm-forming bacterium, GS2, was isolated from the rhizosphere of a sesame plant and subsequently found to produce two quorum-sensing signal molecules that were identified as N-hexanoyl-L-homoserine lactone (m/z 200) and N-octanoyl-L-homoserine lactone (m/z 228) by liquid chromatography-tandem mass spectrometry analysis. The strain was also found to produce IAA (17.2 mu g mL(-1)), gibberellins (113.7 mu g mL(-1)), and ACC deaminase (9.7 mu M alpha-ketobutyrate mg(-1) protein h(-1)). The strain was identified as Serratia glossinae based on a comparison of 16S rRNA gene sequences. Inoculation of the strain promoted growth of a gibberellin-deficient rice dwarf mutant (Waito-C). Different growth attributes, including shoot and root elongation, chlorophyll content, and plant weight could be attributed to the PGPR characteristics of strain GS2. These results suggest that S. glossinae strain GS2 can serve as a microbial agent that improves plant growth.</P>
차체강판 등급에 따른 S-rail 성형성 및 스프링백 특성 비교
정대근(D. G Jung),김흥규(H. K. Kim),김세호(S. H. Kim),김형종(H. J. Kim) 한국소성가공학회 2011 한국소성가공학회 학술대회 논문집 Vol.2011 No.5
In this study, we conducted a series of s-rail forming tests by using sheet sheets with the grades of 340, 440, 590,and 780MPa and then compared formability as well as springback behaviors in the final s-rail products. Various types of drawbeads, i.e. without-bead, locking-bead, and smooth-bead, were applied to the s-rail forming die in order to examine the effect of bead on the s-rail formability and springback. The dimensions of experimentally obtained s-rail products were measured for comparison with finite element predictions. From the comparison, we could confirm that the experimental deformation behaviors agreed well with the finite element predictions in terms of the dependence on sheet sheet grade. The quantitative data of formability and springback for different grades of sheet sheets would be very useful for developing car body parts using high strength steel sheets which are increasingly required by automakers recently.
Hip2 ubiquitin-conjugating enzyme overcomes radiation-induced G2/M arrest
Bae, Y.,Jung, S.H.,Kim, G.Y.,Rhim, H.,Kang, S. Elsevier Biomedical Press 2013 Biochimica et biophysica acta, Molecular cell rese Vol.1833 No.12
Radiation induces cell cycle arrest and/or cell death in mammalian cells. In the present study, we show that Hip2, a ubiquitin-conjugating enzyme, can overcome radiation-induced G2/M cell cycle arrest and trigger the entry into mitosis. Ionizing radiation increased the levels of Hip2 by preventing its degradation but not its gene transcription. The stability of Hip2 in irradiated cells was further confirmed using live cell fluorescence imaging. Flow cytometric and molecular analyses revealed that Hip2 abrogated radiation-induced G2/M arrest, promoting entry into mitosis. Bimolecular fluorescence complementation assays and co-immunoprecipitation experiments showed that Hip2 interacted with and targeted p53 for degradation via the ubiquitin proteasome system, resulting in the activation of cdc2-cyclin B1 kinase to promote mitotic entry. These results contribute to our understanding of the mechanisms that regulate cell cycle progression and DNA damage-induced G2/M checkpoint cellular responses.
Lee, S.G.,Kim, J.,Woo, H.S.,Jo, Y.,Inamdar, A.I.,Pawar, S.M.,Kim, H.S.,Jung, W.,Im, H.S. Elsevier 2014 CURRENT APPLIED PHYSICS Vol.14 No.3
We fabricate a Cu<SUB>2</SUB>ZnSnS<SUB>4</SUB> (CZTS) absorber layer, by using single step electrodeposition of CZTS precursor, deposited at -1.05 V, followed by high temperature annealing in a sulfur atmosphere. X-ray diffraction pattern indicates that the as-grown sample is amorphous in nature, and polycrystalline CZTS thin films with kesterite crystal structure have been obtained by sulfurization from 450 to 580 <SUP>o</SUP>C. Surface morphologies of the as-grown sample show some voids with agglomerated particles. After sulfurization, the morphologies of the annealed samples become more uniform, and dense. EDAX study reveals that the sulfurized samples are nearly stoichiometric, being Cu-rich and S-deficient in composition. The band gaps of the annealed samples are found to be in the range from 1.9 to 1.5 eV.
Rosiglitazone-mediated dendritic cells ameliorate collagen-induced arthritis in mice
Byun, S.H.,Lee, J.H.,Jung, N.C.,Choi, H.J.,Song, J.Y.,Seo, H.G.,Choi, J.,Jung, S.Y.,Kang, S.,Choi, Y.S.,Chung, J.H.,Lim, D.S. Pergamon Press 2016 Biochemical pharmacology Vol.115 No.-
<P>Rosiglitazone is a selective ligand for peroxisome proliferator-activated receptor-gamma (PPAR-gamma), which serves diverse biological functions. A number of autoimmune disease models have been used to examine the anti-inflammatory and immunosuppressive effects of tolerogenic dendritic cells (tDCs). The aim of the present study was to investigate whether rosiglitazone-mediated DC (Rosi-DC) therapy suppressed arthritis in a collagen-induced arthritis (CIA) mouse model. Rosi-DCs were generated by treating immature DCs with TNF-alpha, type II collagen, and rosiglitazone. CIA mice then received subcutaneously (s.c.) two injections of Rosi-DCs. The severity of arthritis was then assessed histopathologically. The phenotypes of the DC and regulatory T (Treg) cell populations in CIA mice were determined by flow cytometry and the effect of Rosi-DCs on the secretion of autoimmunity-inducing cytokines was examined by ELISA. Rosi-DCs expressed lower levels of DC-related surface markers than mature DCs. Histopathological examination revealed that the degree of inflammation in the paws of Rosi-DC-treated mice was much lower than that in the paws of PBS-treated CIA mice. Taken together, these results clearly show that rosiglitazone-mediated DCs ameliorate CIA, most likely via the induction of antigen-specific Treg cells. (C) 2016 Elsevier Inc. All rights reserved.</P>
Kim, S.J.,Lee, J.W.,Jung, Y.S.,Kwon, D.Y.,Park, H.K.,Ryu, C.S.,Kim, S.K.,Oh, G.T.,Kim, Y.C. Elsevier Science Publishers 2009 Journal of hepatology Vol.50 No.6
Background/Aims: Oxidative stress via generation of reactive oxygen species is suggested to be the major mechanism of alcohol-induced liver injury. We investigated the effects of glutathione peroxidase-1 and catalase double deficiency (Gpx-1<SUP>-/-</SUP>/Cat<SUP>-/-</SUP>) on liver injury and changes in the sulfur amino acid metabolism induced by binge ethanol administration. Methods: Ethanol (5g/kg) was administered orally to the wild-type and the Gpx-1<SUP>-/-</SUP>/Cat<SUP>-/-</SUP> mice every 12h for a total of three doses. Mice were sacrificed 6h after the final dose. Results: The Gpx-1/Cat deficiency alone increased malondialdehyde levels in liver significantly. Hepatic methionine adenosyltransferase (MAT) activity and S-adenosylmethionine levels were decreased, however, glutathione contents were not changed. Ethanol administration to the Gpx-1<SUP>-/-</SUP>/Cat<SUP>-/-</SUP> mice increased the elevation of serum alanine aminotransferase activity, plasma homocysteine levels, hepatic fat accumulation and lipid peroxidation compared with the wild-type animals challenged with ethanol. Also the reduction of MAT activity and S-adenosylmethionine levels was enhanced, but MATI/III expression was increased significantly. Conclusions: The results indicate that Gpx-1 and Cat have critical roles in the protection of liver against binge ethanol exposure. Augmentation of ethanol-induced oxidative stress may be responsible for the impairment of the transsulfuration reactions and the aggravation of acute liver injury in the Gpx-1<SUP>-/-</SUP>/Cat<SUP>-/-</SUP> mice.
Lim, S.,Chung, D. R.,Kim, Y. S.,Sohn, K. M.,Kang, S. J.,Jung, S. I.,Kim, S. W.,Chang, H. H.,Lee, S. S.,Bae, I. G. Springer Science + Business Media 2017 Infection Vol.45 No.1
<P>Patients with a prior history of receiving immunosuppressive therapy within 1 month and chronic liver disease have 8.1-fold and 5-fold increased risk of meningitis by L. monocytogenes compared to S. pneumoniae, respectively.</P>
r-Whitney numbers of Dowling lattices
Cheon, G.S.,Jung, J.H. North-Holland Pub. Co ; Elsevier Science Ltd 2012 Discrete mathematics Vol.312 No.15
Let G be a finite group of order m>=1. A Dowling lattice Q<SUB>n</SUB>(G) is the geometric lattice of rank n over G. In this paper, we define the r-Whitney numbers of the first and second kind over Q<SUB>n</SUB>(G), respectively. This concept is a common generalization of the Whitney numbers and the r-Stirling numbers of both kinds. We give their combinatorial interpretations over the Dowling lattice and we obtain various new algebraic identities. In addition, we develop the r-Whitney-Lah numbers and the r-Dowling polynomials associated with the Dowling lattice.
Some combinatorial applications of the q-Riordan matrix
Cheon, G.S.,Jung, J.H. North Holland [etc.] 2015 Linear algebra and its applications Vol.482 No.-
Recently, the authors developed a q-analogue for Riordan matrices by means of Eulerian generating functions of the form g(z)=@?<SUB>n≥0</SUB>g<SUB>n</SUB>z<SUP>n</SUP>/n!<SUB>q</SUB> where n!<SUB>q</SUB> is the q-factorial. We apply this concept to give q-analogues of some familiar objects from the set partitions with double restrictions on blocks, namely the (r,s)-Bessel numbers of both types. By setting r=0 and letting s→~, these numbers may be reduced to the q-Stirling numbers of both kinds. Several algebraic formulas for the q-analogues are also derived using combinatorial methods together with the concept of q-Riordan matrices. In particular, q-analogues of the classical Bessel numbers of both kinds and their combinatorial interpretations are obtained.