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- 저자 : Jukta Adhikari (검색결과 2 건)
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In vitro morphogenesis in Selaginella microphylla (Kunth.) Spring
Jha, Timir Baran,Mukherjee, Siddhartha,Basak, Anusuya,Adhikari, Jukta 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3
Selaginella, an extant genus of primitive vascular plants, has survived over 400 million years of evolution. In vitro morphogenesis in Selaginella microphylla is considered for the first time to establish a well-documented aseptic culture on half- strength Murashige and Skoog's basal medium with 2ip ($4.92-49.21{\mu}M$), or Kn ($4.65-46.47{\mu}M$) or $GA_3$ ($2.89-28.90{\mu}M$) for shoot multiplication, and with different concentrations of IBA (4.9-49 lm) to initiate root cultures. $GA_3$ was instrumental for shoot multiplication as well as induction of reproductive structures in each and every leaf axil. On the other hand, it is observed that IBA alone in S. microphylla can act as signal molecules for induction of enormous numbers of root masses from a few existing roots. An interesting pattern of re-differentiation has also been observed where apical portions of large numbers of roots were converted to green shoot apical meristems. Further differentiation produced tiny green shoots. Distinct bipolarity was noted in shoots when they were isolated from root masses and appeared as embryo-like structures. Chromosome analysis from in vitro sporophytic plants revealed 2n = 16 chromosomes, indicating chromosomal stability. The interesting in vitro pattern of morphogenesis obtained in S. microphylla may provide new insights into totipotency of plants.
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In vitro morphogenesis in Selaginella microphylla (Kunth.) Spring
Timir Baran Jha,Siddhartha Mukherjee,Anusuya Basak,Jukta Adhikari 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3
Selaginella, an extant genus of primitivevascular plants, has survived over 400 million years ofevolution. In vitro morphogenesis in Selaginella microphyllais considered for the first time to establish a welldocumentedaseptic culture on half- strength Murashigeand Skoog’s basal medium with 2ip (4.92–49.21 lM), orKn (4.65–46.47 lM) or GA3 (2.89–28.90 lM) for shootmultiplication, and with different concentrations of IBA(4.9–49 lm) to initiate root cultures. GA3 was instrumentalfor shoot multiplication as well as induction of reproductivestructures in each and every leaf axil. On the otherhand, it is observed that IBA alone in S. microphylla canact as signal molecules for induction of enormous numbersof root masses from a few existing roots. An interestingpattern of re-differentiation has also been observed whereapical portions of large numbers of roots were converted togreen shoot apical meristems. Further differentiation producedtiny green shoots. Distinct bipolarity was noted inshoots when they were isolated from root masses andappeared as embryo-like structures. Chromosome analysisfrom in vitro sporophytic plants revealed 2n = 16 chromosomes,indicating chromosomal stability. The interesting invitro pattern of morphogenesis obtained in S. microphyllamay provide new insights into totipotency of plants.
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