Pharbitis nil 개화 유도시 (誘導時) 엽단백질 변화에 대한 면역학적 분석

맹주선 (Jue Son Maeng) 한국식물학회 1982 Journal of Plant Biology Vol.25 No.4

Using double immunodiffusion and immunoelectrophoretic techniques, attempts were made to detect any protein changes in leaf tissues of a short-day plant, Pharbitis nil Chois, variety Violet during floral induction under 8 hr light, 16 hr dark cycles. Immunoprecipitin systems showed at least four proteins newly appeared in the induced leaf tissues. Accumulation of the proteins were observed as the induction proceeded.

Lemna gibba G3 개화 ( 꽃눈분화 ) 에 대한 Salicylic Acid 와 Dimethylsulfoxide 의 영향

맹주선(Jue Son Maeng),배정명(Jung Myung Bae) 한국식물학회 1984 Journal of Plant Biology Vol.27 No.4

The reversal effect of salicylie acid(SA) On inhibition of flowering in Lemna gibba G3 grown on NH_4^+-free 1/2H medium under continuous light is modified by PO_4^2- and Ca^2+ levels. Dimethylsulfoxide(DMSO) either depresses the SA effect in NH_4^+-free 1/2H medium or amplifies it in E medium. The dual action of DMSO determined by relative levels of macro-and micronutrient components is discussed.

무기질소원의 종류에 따른 남조류 (藍藻類) Anabaena flos - aquae 광합성의 특성

맹주선 (Jue Son Maeng) 한국식물학회 1982 Journal of Plant Biology Vol.25 No.4

The kinetics of ^14C fixation have been investigated in Anabaena flos-aquae growing on NH^+_4, NO^-_3 and N_2-N in batch cultures. Growth rate was highest with NH^+_4, followed by NO^-_3 and finally N_2. The compensation intensity(I_0) and the half-saturation irradiance(K_1) with N_2 were higher than with other N sources, but the maximum C fixation rate(P_max) was lower. The P_max/K_1 ration, which is analogous to quantum efficiency at low irradiance ranges, was also lower with N_2. All these parameters except K_1 decrease with culture age, or decreasing growth rate. Since ^14C uptake measures net photosynthesis, the higher values of I_0 and K_1, and the low values of P_max and P_max/K_1 ratio with N_2 appear to be related to the high energy demand of N_2 fixation. They may also be related to the low maximum growth rate with N_2-N.

光週期植物의 開花誘導에 미치는 2-Chloroethylphosphonic Acid(Ethephon)의 效果에 關한 硏究 : Ⅰ.Ethephon에 依한 Lemna perpusilla 6746 開花抑制 Ⅰ. Inhibition of Flowering in Lemna perp

孟柱善 한국식물학회 1977 Journal of Plant Biology Vol.20 No.2

The inhibitory effect of ethephon on the flowering in Lemna perpusilla 6746 was shown to be related to sucrose concentrations and dilution factors of Hutner's medium. When grown in 1/10-strength Hutner's medium under 10(14) cycles, the plants have been completely inhibited in the floral induction by ethephon(>5ppm) in the presence of sucrose(>20 mM) in the medium. However, in a less diluted Hutner's medium (1/2-strength), the inhibition of flowering by ethephon was observed to be partially diminished by sucrose at a high concentration(30 mM), while a low concentration of sucrose enhanced the inhibitory effect of ethephon in flowering. As inductive dark periods were extended, the effects of both compounds were partially nullified. Since no significant amount of ethylene possibly released in ethephon decomposition in the medium was detected, the inhibitory effect of ethephon in flowering was postulated to be exerted only through ethylene production within the plants. Plants were incubated in 10 ppm ethephon-containing medium during either dark or light periods, singly or periodically. The most effective single treatment with ethephon was observed during the 4th dark period, when formation of floral stimulus was assumed to be completed beyond a critical level. This postulation can be partially supported by a fact that the plants should be exposed to at least more than four consecutive 10(14) cycles for flowering.

CLONGING OF GENES EXPRESSED UPON FLORAL INDUCTION IN PHARBITIS COTYLEDONS

Kim, Kang-Chang,Hur, Yoon-Kang,Maeng, Jue-Son Korean Society of Photoscience 1998 Journal of Photosciences Vol.5 No.3

Using differential display reverse transcription technique, the present study attempted to isolate and characterize genes specifically expressed in cotyledons of Pharbitis nil Choisy cv. Violet during floral induction. A total of 107 bands specific to the inductive condition were initially obtained with 80 primer sets of 20 different arbitrary primers combined with 4 kinds of T12MN. In northern blot analysis with reamplified cDNAs as probes, three cDNAs were detected to be expressed specificcally in the induced cotyledon tissues, and designated PnFL-1, PnFL-2 and PnFL-3 , the size of which were 228 bp, 317 bp and 272 bp, respectively. A search for sequences similar to the decuced amino acid sequences was conducted using GenBank and EMBL database ; seequence encoded by PnFL-1 had 29% identity with the clone of Arabidopsis thaliana similiar to reverse trascriptase (Genbank Acc. N0.3047086), PnFL-2 shared 50% identity with hydroxiyproline-rich glycoprotein of Glycine max(GenBank Acc. No.347455), and PnFL-3 had 46% identity with TAMU 4. Thaliana genomic clone T23E16 (GenBank Acc. No.B67574). None of them was known gene in the plant system up to date, implying that the fragments may comprise parts of genes which are associated with the floral induction in Pharbitis nil.

Expression Characteristics of Pokeweed Antiviral Proteins(PAPs)

Hur, Yoon Kang,Han, Ching Tack,Maeng, Jue Son 한국식물학회 1997 Journal of Plant Biology Vol.40 No.1

좀개구리밥 ( Lemna gibba G3 ) 의 개화에 미치는 Polyamine 의 영향

김강창(Kang Chang Kim),맹주선(Jue Son Maeng) 한국식물학회 1992 Journal of Plant Biology Vol.35 No.4

The flowering in Lemna gibba G3, a long-day plant, was promoted under continuous light by agmatine, putrescine, spermidine and spermine present in the culture medium. Methylglyoxal-bis (guanylhydrazone) (MGBG) and cyclohexylamine (CHA), inhibitors of polyamine biosynthesis, were found to suppress the flowering in the plants. The vegetative growth rate was kept constant while the flowering was being promoted by the polyamines, and the inhibitors with depressive effect on flowering showed stimulatory effect on vegetative growth. The pattern of vegetative growth during floral promotion or depression was an indication that the promotive action of the polyamines and the suppressive effect of the inhibitors may be outcome of their possible involvement specifically in the flowering process rather than in broad spectrum of growth of L. gibba G3. The degree of promotive action of spermdine and spermine could not be altered (or lessened) by simultaneous application of their inhibitors to the medium. This phenomenon indicates that the flowering process in L. gibba G3 may largely be dependent to the status of endogenous spermidine and spermine. Endogenous level of spermidine in florally induced Lemna, was found to rapidly increase. In 24 h of floral induction, the content reached at the level 2 times higher than that in non-induced plants. The elevated level of spermidine provides an additional, though premature, evidence supporting the postulation that endogenous polyamine status might play an important role in the very early stage of floral induction in L. gibba G3.

좀개구리밥 ( Lemna gibba G3 ) 원형질막의 투과성 변화와 관련된 막전위의 특성

윤병호(Byung Gil Yoon),맹주선(Jue Son Maeng) 한국식물학회 1990 Journal of Plant Biology Vol.33 No.2

The membrane potential in the subepidermal cells of Lemma gibba G3 fronds was measured in the dark with glass capillary microelectrodes. At pH 7, the membrane potential, approximately-215mV, could be depolarized to -82~-88mV by 0.1 mM dicyclohexylcarbodiimide (DCCD) or by KCN at 0.3 mM or higher concentrations. When the pH of the medium was altered the potential showed reversible changes, while it revealed no response to the external pH changes when energy transduction across the membrane was being blocked by 0.1 mM DCCD. The results support an assumption that the active component of the membrane potential of Lemma subepidermal cells is generated by electrogenic H+-pump. By the addition of 0.10~5.00mM salicylic acid (SA) to the bathing medium the membrane potential was depolarized to a great extent, and the removal of SA from the medium repolarized the potential showing almost complete recovery, 92.3~97.6% to the initial levels. Although the potential was greatly depolarized by 5.0% or higher concentrations of dimethylsulfoxide (DMSO), the recovery rate by DMSO removal was decreased as the pretreatment concentration had increased. Twenty percent DMSO pretreatment limited the recovery at only 47.1%. The presence of SA in the bathing medium could reversibly increase the permeability of the plasmalemma. DMSO at its concentration of 5.0% or higher increased the permeability of the membrane by irrevesibly impairing the membrane component involved in the membrane permeability.

대청호의 수질과 식물플랑크톤 현존량

이진환,오희목,맹주선 한국환경생물학회 2000 환경생물 : 환경생물학회지 Vol.18 No.3

본 연구는 1998년 5월부터 11월까지 대청호의 2개 정점 표·중·저층에서 수질과 일차생산을 나타내는 식물플랑크톤 현존량과의 관계를 규명하기 위하여 물리·화학적 환경요인(수온, 용존산소량, pH, 투명도, 총질소, 질산염, 아질산염, 암모니아, 총인, 인산염, 용존총인, 총유기탄소, 부유물질), 식물플랑크톤 현존량, chlorophyll-α의 농도를 조사하였다. 영양염류(아질산염, 질산염, 암모니아 및 인산염)가 과거에 비하여 증가되었으며, 총질소, 총인, chlorophyll-α 및 투명도는 부영양화의 기준치를 초과하여 대청호의 수질은 심한 부영양화 수역으로 판명되었다. 식물플랑크톤 현존량은 7월과 8월의 정점 1을 제외하고 년중 대발생을 유지하고 있었으며, 봄철 대발생은 아질산염, 질산염 및 인산염의 영향이 큰 것으로 판명되었다. Chlorophyll-α의 농도는 식물플랑크톤 현존량의 월별 변동과 대부분 같은 경향으로 변화하였다. 여름철에 높은 부유물질은 장마기의 강우에 따른 것으로 판단되며, 특히 8월의 많은 종속영양 세균수는 높은 수온과 장마시 주변 수역으로부터 유입된 각종 유기영양염류의 유입에 따른 대량증식으로 추정된다. In order to study the relationship between water quality and phytoplankton standing crops, the authors investigated the physicochemical factors, phytoplankton standing crops and chlorophyll-α at two stations in the Daechung Reservoir during the period from May to November 1998. Nutrients(nitrite, nitrate, ammonia and phosphate) have gradually increased more than those of the previous reports. Concentrations of the total nitrogen, phosphorus, chlorophyll-α and transparency exceeded the standard value of eutrophication. It could be assumed that the eutrophication of the Dechung reservoir was caused by various pollutants. Chlorophyll-α concentration was under the influence of phytoplankton standing crops, but very tiny cell-sized blue-green algae were less important in regards to the chlorophyll-α. Except at station 1 from July to August, phytoplankton standing crops bloomed during the time of the survey. Spring phytoplankton blooms caused by nitrate and phosphate in the surface layer. High concentrations of the suspended solids in July and August were caused by an inflow of them due to heavy rains. Density of heterotrophic bacteria was high in August owing to the high water temperatures and the organic nutrients which were from the nearby reservoir.

Polyamine 과 Ethylene 영향하에서의 나팔꽃 자엽내 광조기성 (光調期性) 개화유도

김강욱(Kang Chang Kim),맹주선(Jue Son Maeng) 한국식물학회 1995 Journal of Plant Biology Vol.38 No.3

Exogenous putrescine of 0.5 mM or higher concentrations applied during a 16 h inductive dark period could elevate putrescine content in cotyledons of Pharbitis nil choisy cv. Violet, a short-day plant, resulting in complete blocking of photoperiodic floral induction. Titers of putrescine, spermidine and spermine in the cotyledons were traced throughout a 16 h dark period. While non-induced cotyledons under continuous light slightly increased levels of polyamines, induced tissue maintained its putrescine, spermidine and spermine levels as low as 66.4%, 60.9% and 84.9% of non-induced levels respectively. Endogenous polyamines kept at lower levels in the inductive dark period were found to upsurge by a night break treatment of 10 min light in the middle of the dark and consequently the inductive dark effect was canceled. Elevation of polyamine titers could also be induced by 100 μL/L ethylene treatment which completely suppressed floral induction. Compared to untreated cotyledons, ethylene-treated tissues increased putrescine content by as much as 136.5% in 12 h and spermidine level by up to 130.1% in 8 h. Ethylene-treated cotyledons not only increased endogenous polyamine content but also liberate ethylene in the second half of the inductive dark period accumulating up to three to fourfold level supporting a hypothesis that ethylene-treated tissues are stimulated to produce ethylene which in turn accelerates polyamine biosynthesis in the tissues. It is postulated that substantially low polyamine titers in the inductive dark period would be one of the necessary factors controlling photoperiodic induction of flowering in Pharbitis nil and the inhibitory effects of night break and exogenous ethylene treatment may be attributed to their action to stimulate endogenous polyamine production.