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RISS 인기검색어
- 검색키워드
- 저자 : Jos Thomas Puthur (검색결과 3 건)
- 무료
- 기관 내 무료
- 유료
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High Frequency In Vitro Regeneration of Kigelia pinnata L. Via Organogenesis
Puthur, Jos T.,Thomas, T. Dennis 한국식물학회 2004 Journal of Plant Biology Vol.47 No.1
An effective and reproducible protocol for the micropropagation of Kigelia pinnata L through high frequency callus regeneration is described. Seeds were surface sterilized before culturing on Gamborgs basal medium (B5 medium). After two weeks the cotyledonary node along with a portion of the hypocotyl were carefully excised from well-developed embryos and subcultured on B5 medium supplemented with different concentrations of 2,4-D and BAP. The cultured cotyledonary node explants showed callus formation at the base of the lower cut end of the hypocotyl. This callus showed shoot initiation after two weeks of subculture on the regeneration medium supplemented with various concentrations of BAP alone or in combination with NAA. The highest number of shoot regeneration occurred on medium containing 5 μM BAP and 0.1 μM NAA. The optimum rooting of the regenerated shoots was observed on %B5 medium supplemented with 4 μM IBA. Micropropagated plants were successfully established in soil in field condition with a survival frequency of 100%.
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Kolothodi Chandran Jisha,Jos Thomas Puthur 한국작물학회 2014 Journal of crop science and biotechnology Vol.17 No.4
Seed priming improves the seed performance and also helps the seedlings to alleviate the detrimental effects of various stresses. Seed priming is believed to bring about some biochemical changes in the metabolism within the seed, which ultimately favors germinationand the further growth stages of the seedlings even under stressed conditions. The present investigation was carried out tostudy the effect of hydropriming and halopriming in three rice varieties (Neeraja, Vaisakh, and Vytilla 6), with varied abiotic stresstolerance potential under NaCl and PEG stress. In general, the application of both stresses, NaCl and PEG induced retardation ofgrowth and metabolism of the seedlings. However, seed priming treatments could reduce the extent of decrease in these biologicalattributes. Both hydro- and halopriming resulted in the enhancement of protein, carbohydrate, and photosynthetic pigment content,modulated antioxidant enzyme activities, reduced the lipid peroxidation of biomembranes, and enhanced the photochemistry andmitochondrial activities in rice seedlings subjected to NaCl and PEG stress as compared to non-primed ones. According to the variousmorphological, physiological, and biochemical characteristics studied in the rice seedlings raised from primed and non-primedseeds, we confirmed that both hydropriming and halopriming had a positive influence on stimulating metabolism in rice seeds, whichultimately resulted in improved seedling vigor and tolerance under NaCl and PEG stress. Halopriming was found to be more efficientthan hydropriming in enhancing the seedling vigor, overall growth, and stress tolerance potential of rice varieties
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Comparative Study on the Zinc and Cadmium Tolerance Potential of Twelve Prominent Rice Cultivars
Asari Kandi Sinisha,Jos Thomas Puthur 한국작물학회 2018 Journal of crop science and biotechnology Vol.21 No.3
Heavy metal pollution being a potential threat to agriculture raising rice cultivars with heavy metal tolerance is a promising strategy for remediation of heavy metal polluted agricultural lands. We present here a comprehensive study describing the differences in physiological and biochemical responses of 12 prominent high-yielding rice cultivars to increasing ZnSO4 concentrations (0, 2, 6, and 10 mM) and CdCl2 concentrations (0, 1, 2, and 3 mM). Even though Zinc (Zn) is an essential element required for the normal growth and development process of plants, a higher concentration of Zn has an antagonistic effect. Cadmium (Cd) is detrimental to plants and is found in soils contaminated with heavy metals. The effects of Zn and Cd on rice seedlings were a reduction in shoot length, greater chlorophyll and carotenoid loss, higher malondialdehyde content, proline accumulation, and an increased level of sugar and amino acids when treated with CdCl2 and ZnSO4. The CdCl2 and ZnSO4 stress-induced biochemical changes displayed major differences in the 12 rice cultivars in terms of tolerance to Zn and Cd toxicity. Our data provides evidence that the cultivar Varsha showed the highest tolerance and cultivar JY showed the least tolerance towards Cd and Zn toxicity.
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