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        Separation of Taxanes from Taxus canadensis Using Dynamic Pressurized Liquid Extraction

        Yuheng Wang,Joanne Gamage,Zisheng Zhang 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.4

        An extraction technique, dynamic pressurized liquid extraction (DPLE), was proposed to extract the taxanes; including 10-DAB III, Baccatin III, 9-DHB III and paclitaxel, from powdered Taxus canadensis needles. A dual-solvent approach was adopted in which the impurities were firstly removed by extraction with hexane,and the taxanes were subsequently extracted with an appropriate solvent. The performance of chloroform, dichloromethane,and mixtures of methanol/dichloromethane was compared for use as the taxane-extracting solvent, and it was found that solvents containing a higher proportion of methanol had higher extraction capabilities. The effect of temperature on DPLE extraction of the taxanes was also studied, and it was found that higher extraction efficiencies could be realized with increasing temperature up to a threshold of 90℃. Based on a progressive conversion model, a kinetic equation for the extraction process was proposed. This model successfully confirmed that smaller needle powder particle sizes would result in higher extraction rates, which is consistent with the data obtained by experimentation.

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