Tumour-Derived Reg3A Educates Dendritic Cells to Promote Pancreatic Cancer Progression

Guo, Jie,Liao, Mengfan,Hu, Xianmin,Wang, Jun Korean Society for Molecular and Cellular Biology 2021 Molecules and cells Vol.44 No.9

As a pancreatic inflammatory marker, regenerating islet-derived protein 3A (Reg3A) plays a key role in inflammation-associated pancreatic carcinogenesis by promoting cell proliferation, inhibiting apoptosis, and regulating cancer cell migration and invasion. This study aimed to reveal a novel immuno-regulatory mechanism by which Reg3A modulates tumour-promoting responses during pancreatic cancer (PC) progression. In an in vitro Transwell system that allowed the direct co-culture of human peripheral blood-derived dendritic cells (DCs) and Reg3A-overexpressing/ silenced human PC cells, PC cell-derived Reg3A was found to downregulate CD80, CD83 and CD86 expression on educated DCs, increase DC endocytic function, inhibit DC-induced T lymphocyte proliferation, reduce IL-12p70 production, and enhance IL-23 production by DCs. The positive effect of tumour-derived Reg3A-educated human DCs on PC progression was demonstrated in vivo by intraperitoneally transferring them into PC-implanted severe combined immunodeficiency (SCID) mice reconstituted with human T cells. A Reg3A-JAK2/STAT3 positive feedback loop was identified in DCs educated with Reg3A. In conclusion, as a tumour-derived factor, Reg3A acted to block the differentiation and maturation of the most important antigen-presenting cells, DCs, causing them to limit their potential anti-tumour responses, thus facilitating PC escape and progression.

In Vitro Biological Characterization of DCUN1D5 in DNA Damage Response

Guo, Wei,Li, Guo-Jun,Xu, Hong-Bo,Xie, Jie-Shi,Shi, Tai-Ping,Zhang, Sheng-Zhong,Chen, Xiao-Hong,Huang, Zhi-Gang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Background: Novel prognostic biomarkers or therapeutic molecular targets for laryngeal squamous cell carcinoma (LSCC) are an urgent priority. We here sought to identify multiple novel LSCC-associated genes. Methods: Using high-density microarray expression profiling, we identified multiple genes that were significantly altered between human LSCCs and paired normal tissues. Potential oncogenic functions of one such gene, DCUN1D5, were further characterized in vitro. Results: Our results demonstrated that DCUN1D5 was highly expressed in LSCCs. Overexpression of DCUN1D5 in vitro resulted in 2.7-fold increased cellular migration, 67.5% increased invasive capacity, and 2.6-fold increased proliferation. Endogenous DCUN1D5 expression was decreased in a time-dependent manner after genotoxic stress, and silencing of DCUN1D5 by siRNA decreased the number of cells in the S phase by 10.2% and increased apoptosis by 11.7%. Conclusion: Our data suggest that DCUN1D5 in vitro might have vital roles in DNA damage response, but further studies are warranted to assess its significance in vivo.

Meta-analysis of the Relation Between the VDR Gene TaqIpolymorphism and Genetic Susceptibility to Prostate Cancer in Asian Populations

Guo, Ya-Jie,Shi, Ze-Ming,Liu, Jun-Da,Lei, Ning,Chen, Qiu-Hong,Tang, Ying Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

Background: Polymorphisms of the Taq I gene have been associated with prostate cancer risk. Methods:We applied a fixed-effects model to combine odds ratios (ORs) and 95% confidence intervals (95% CI). The Egger's test was carried out to evaluate potential publication bias. Results: A total of 10 case-control studies enrolling 1,141 prostate cancer patients and 1,685 controls were included in this meta-analysis. Compared with the T allele, the OR for the C allele was 0.81 (0.70-0.94). The ORs for CT and CC+CT genotypes were 0.86 (0.74-1.01) and 0.84 (0.73-0.97) compared to wide type genotype (homozygote TT). Conclusions: The present meta-analysis suggests that the TF gene Taq I polymorphism may reduce the prostate cancer risk in Asian populations.

Somatostatin Analogues Do Not Prevent Carcinoid Crisis

Guo, Lin-Jie,Tang, Cheng-Wei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.16

Background: Carcinoid crisis is a life-threating syndrome of neuroendocrine tumors (NETs) characterized by dramatic blood pressure fluctuation, arrhythmias, and bronchospasm. In the era of booming anti-tumor therapeutics, this has become more important since associated stresses can trigger carcinoid crisis. Somatostatin analogues (SSTA) have been recommended for prophylactic administration before intervention procedures for functioning NETs. However, the efficacy is still controversial. The aim of this article is to review efficacy of SSTA for preventing carcinoid crisis. Materials and Methods: PubMed, Cochrane Controlled trials Register, and EMBASE were searched using 'carcinoid crisis' as a search term combining terms with 'somatostatin'; 'octreotide'; 'lanreotide' and 'pasireotide' until December 2013. Results: Twenty-eight articles were retrieved with a total of fifty-three unique patients identified for carcinoid crisis. The most common primary sites of NETs were the small intestine and respiratory tract. The triggering factors for carcinoid crisis included anesthesia/surgery (63.5%), interventional therapy (11.5%), radionuclide therapy (9.6%), examination (7.7%), medication (3.8%), biopsy (2%) and spontaneous (2%). No randomized controlled trials (RCTs) were identified and two case-control studies were included to assess the efficacy of SSTA for preventing carcinoid crisis by meta-analysis. The overall pooled risk of perioperative carcinoid crisis was similar despite the prophylactic administration of SSTA (OR 0.44, 95% CI: 0.14 to 1.35, p=0.15). Conclusions: SSTA wasnot helpful for preventing carcinoid crisis based on a meta-analysis of retrospective studies. Attentive monitoring and careful intervention are essential. Future studies with better quality are needed to clarify any effect of SSTA for preventing carcinoid crisis.

Tan Lejos, Tan Cerca: On the Intellectual Implications of Latin American Studies in East Asia

Guo Jie 한국라틴아메리카학회 2021 라틴아메리카연구 Vol.34 No.1

The Pacific Ocean separates East Asia and Latin America but also opens a window of opportunity for exchanges between the two regions. Due to the rapid development of bilateral economic relations, increased government research funding and ever deepening intellectual curiosity among scholars, the development of Latin American Studies in China has seen unprecedented developments over the past two decades. Japan and South Korea also have a long history of engagement with Latin America, and research communities in both countries have already developed distinct traditions and approaches to studying the region. With the globalisation of Latin American Studies, East Asian scholars face common challenges and responsibilities as they make important decisions about the way they view the North Atlantic research tradition and refine their identity or cultural consciousness as the subject of the “South” in the cognitive system of the global frame. In 2014, East Asian Network of Latin American Studies (EANLAS), a regional academic community promoted by the joint efforts of Latin American scholars from Japan, South Korea and China, is expected to serve the realization of the above visions at the entity level through communication and collaboration with relevant academic networks at various levels.

Two-Step Oxidation of Refractory Gold Concentrates with Different Microbial Communities^s

( Guo-hua Wang ),( Jian-ping Xie ),( Shou-peng Li ),( Yu-jie Guo ),( Ying Pan ),( Haiyan Wu ),( Xin-xing Liu ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.11

Bio-oxidation is an effective technology for treatment of refractory gold concentrates. However, the unsatisfactory oxidation rate and long residence time, which cause a lower cyanide leaching rate and gold recovery, are key factors that restrict the application of traditional bio-oxidation technology. In this study, the oxidation rate of refractory gold concentrates and the adaption of microorganisms were analyzed to evaluate a newly developed two-step pretreatment process, which includes a high temperature chemical oxidation step and a subsequent bio-oxidation step. The oxidation rate and recovery rate of gold were improved significantly after the two-step process. The results showed that the highest oxidation rate of sulfide sulfur could reach to 99.01 % with an extreme thermophile microbial community when the pulp density was 5%. Accordingly, the recovery rate of gold was elevated to 92.51%. Meanwhile, the results revealed that moderate thermophiles performed better than acidophilic mesophiles and extreme thermophiles, whose oxidation rates declined drastically when the pulp density was increased to 10% and 15%. The oxidation rates of sulfide sulfur with moderate thermophiles were 93.94% and 65.73% when the pulp density was increased to 10% and 15%, respectively. All these results indicated that the twostep pretreatment increased the oxidation rate of refractory gold concentrates and is a potential technology to pretreat the refractory sample. Meanwhile, owing to the sensitivity of the microbial community under different pulp density levels, the optimization of microbial community in bio-oxidation is necessary in industry.

Expression of Human Stem Cell Factor with Recombinant Baculovirus in BmN Cell Line and Silkworm

( Guo Xi Jie ),( Jin Yong Feng ),( Yang Ming Guan ),( Zhang Yao Zhou ) 한국잠사학회 2002 International Journal of Industrial Entomology Vol.4 No.1

Species Classification and Quality Assessment of Chaihu (Radix Bupleuri) Based on High-Performance Liquid Chromatographic Fingerprint and Combined Chemometrics Methods

Xiao-Jie Li,Jie Hu,Zhen-Yu Li,Xue-Mei Qin,Li-Zeng Zhang,Xiao-Qing Guo 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.6

A high-performance liquid chromatographic (HPLC) method was established to analyze 36 Chaihu (Radix Bupleuri) samples collected from three species (Bupleurum chinense DC., B. scorzonerifolium Willd. and B. smithii Wolff.). Addition of trifluoroacetic acid into the mobile phase resulted in fingerprint chromatograms with stable baselines. There were thirty-two characteristic peaks in the standard fingerprint of B. chinense DC. Different recognition pattern methods, including similarity analysis (SA), hierarchical cluster analysis (HCA), principal component analysis (PCA) and partial least squares-discrimination analysis (PLS-DA) were utilized to analyze the 36 samples based on the contents of chemical constituents. Consistent results from SA, HCA and PCA analysis illustrated the rationalisation for why B. smithii Wolff. was not quoted in the Chinese Pharmacopoeia and classified samples were in agreement with their species. PLS-DA loading plots showed the chemical markers which had the most influences on the separation among different species. However, SA, HCA and PCA could not differentiate between wild and cultivated B. chinense DC. as well as between samples from different provinces. HPLC fingerprint in combination with chemometric techniques provided a very flexible and reliable method for homogeneity evaluation and quality assessment of traditional Chinese medicine.

A comparative study of landslide susceptibility maps using logistic regression, frequency ratio, decision tree, weights of evidence and artificial neural network

Liang-Jie Wang,Min Guo,Kazuhide Sawada,Jie Lin,Jinchi Zhang 한국지질과학협의회 2016 Geosciences Journal Vol.20 No.1

For the purpose of comparing susceptibility mapping methods in Mizunami City, Japan, the landslide inventory was partitioned into three groups as various training and test datasets to identify the most appropriate method for creating a landslide susceptibility map. A total of fifteen landslide susceptibility maps were produced using frequency ratio, logistic regression, decision tree, weights of evidence and artificial neural network models, and the results were assessed using existing test landside points and areas under the relative operative characteristic curve (AUC). The validation results indicated that the logistic regression model could provide the highest AUC value (0.865), and a relatively high percentage of landslide points fell in the high and very high landslide susceptibility classes in this study. Furthermore, the paper also suggested that the model performances would be increased if appropriate landslide points were used for the calculation.

중국 산시성(山西省)지역 관제묘건축의 지붕의장에 관한 연구

곽항걸(Guo, Heng Jie),이건하(Lee, Keon-ha),김영은(Kim, Young-eun) 대한건축학회 2014 대한건축학회논문집 Vol.30 No.12

Shanxi province was the hometown of GuanDi. The analysis of community features that found in the roof of common GuanDi Temple show as follows: 1. There are many differences in the roof design of Door, Theater Stage, Pray Hall, Main Hall, Spring and Autumn Palace between Shanxi province and Henan province. 2. The Ornament on roof ridge mostly use patterns of dragon head and dragon tail in Shanxi province. The Ridge brake use various patterns in Shanxi province. 3. Deity carving appears in the Dragon ridge and Vertical ridge. Animal carving appears in the Dragon ridge, Vertical ridge and Descending ridge in Shanxi province. 4. Decorations such as Dragon carving, Peony blossom carving, Flowering plant carving and Word carving are used in the surface of Dragon ridge. Decorations such as Dragon carving and Peony blossom carving are used in the surface of Vertical ridge. Decorations such as Peony blossom carving and Flowering plant carving are used in the surface of Descending ridge.