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Jian-Jiang Zhong,Wei Wang,Zhen-Jiang Zhao,Yufang Xu,Xuhong Qian 한국생물공학회 2005 Biotechnology and Bioprocess Engineering Vol.10 No.2
A series of fluorine and hydroxyl containing jasmonate derivatives, which were chemically synthesized in our institute, were investigated for their effects on the biosynthesis and heterogeneity of ginsenosides in suspension cultures of Panax notoginseng cells. Compared to the control (without addition of elicitors), 100 M of each of the jasmonate was added on day 4 to the suspension cultures of P. notoginseng cells. It was observed that, jasmonates greatly enhanced the ginsenoside content and the ratio of Rb group to Rg group (i.e. (Rb1 + Rd)/(Rg1 + Re)) in the P. notoginseng cells. Some of the synthetic jasmonates, such as pentafluoropropyl jasmonate (PFPJA), 2-hydroxyethyl jasmonate (HEJA) and 2-hydroxye-thoxyethyl jasmonate (HEEJA), could promote the ginsenoside content to 2.55 0.11, 3.65 0.13 and 2.94 0.06 mg/100 mg DW, respectively, compared to that of 0.64 0.06 mg/100 mg DW for the control and 2.17 0.04 mg/100 mg DW by the commercially available methyl jasmonate (MJA); and they could change the respective Rb:Rg ratio to 1.60 0.04, 1.87 0.01 and 1.56 0.05, compared to that of 0.47 0.01 for the control and 1.42 0.06 by MJA. The results suggest that suitable esterification of MJA with fluorine or hydroxyl group could increase the elicitation activity to induce plant secondary metabolism. The information obtained from this study is useful for hyper-production of heterogeneous products by plant cell cultures.
Recent advances in bioreactor engineering
Jian-Jiang Zhong 한국화학공학회 2010 Korean Journal of Chemical Engineering Vol.27 No.4
A bioreactor is the core of biological processes. To design an appropriate bioreactor system for a particular bioprocess, intensive studies on the biological system, such as cell growth, metabolism, genetic manipulation, and protein or other product expression are needed to understand the cells’ requirement on their physical and chemical environments. It is also necessary to control and optimize the bioreactor environment via operating variables in order to favor the desired functions of cells and achieve cost-effective large-scale manufacture. This article briefly describes fundamental design principles and new types of bioreactors such as centrifugal impeller and wave bioreactors. Bioreactor operation factors and modes including mixing, oxygen supply, shear force, fed-batch, and perfusion cultures are discussed. The trends in bioreactor engineering are also briefly shown.
Zhong, Jian-Jiang,Fang, Qing-Hua,Tang, Ya-Jie The Korean Society of Plant Biotechnology 2002 Plant molecular biology and biotechnology research Vol.4 No.3
Submerged cultures of Ganoderma lucidum, a valuable mushroom in traditional Chinese medicine, were used for production of bioactive Banoderic acids and Ganoderma polysaccharides. The significant effects of oxygen supply were demonstrated in both shake flasks and bioreactors. By changing the medium loading volume in a shake flask, a different value of initial volumetric oxygen transfer coefficient ($K_L$a) was obtained, and a higher $K_L$a value led to a higher biomass density and a higher productivity of both intracellular polysaccharide and ganoderic acid. In a stirred bioreactor, at an initial $K_L$a of 78.2 $h^{-1}$, a maximal cell concentration of 15.6 g/L by dry weight was obtained, as well as a maximal intracellular polysarcharide (IPS) production of 2.2 g/L and its maximal productivity of 220 mg/(L.d). An increase of initial $K_L$a led to a higher production and productivity of GA, and the GA production and productivity at an initial $K_L$a of 96.0 $h^{-1}$ was 1.8-fold those at an initial $K_L$a of 16.4 $h^{-1}$. The fundamental information obtained in this study may be useful for efficient large-scale production of these valuable bioactive products by the submerged cultures.
Jiang, Ai-Ren,Gao, Chang-Ming,Ding, Jian-Hua,Li, Su-Ping,Liu, Yan-Ting,Cao, Hai-Xia,Wu, Jian-Zhong,Tang, Jin-Hai,Qian, Yun,Tajima, Kazuo Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.1
To evaluate the relationship between abortions and risk of breast cancer, we conducted a case-control study with 669 cases and 682 population-based controls in Jiangsu Province of China. A structured questionnaire was used to elicit detailed information. Unconditional logistic regression analysis was performed to calculate odds ratios (ORs) and 95% confidence intervals (CIs). The results have revealed that induced abortion was related to increased risk of breast caner. Premenopausal women who had ${\geq}3$ times of induced abortion were at increased crude OR (2.41, 95%CI: 1.09-5.42) and adjusted-OR (1.55, 95%CI: 1.15-5.68). Postmenopausal women with a previous induced abortion were at increased crude OR (2.04, 95%CI: 1.48-2.81) and adjusted-OR (1.82, 95%CI: 1.30-2.54), and there was a significant increase trend in OR with number of induced abortions (p for trend: 0.0001). Overall, spontaneous abortion did not significantly alter the risk of breast cancer, but postmenopausal women who had history of spontaneous abortion were at increased OR. These results suggested that relationship between breast cancer and abortions may depend on menopausal status and induced abortion may played an important role in the development of breast cancer in Jiangsu' women of China.