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- 저자 : Ji Yye Lee(이지예) (검색결과 1 건)
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Yu Rim Lee(이유림),Soo Youn Lee(이수연),Ji Yye Lee(이지예),HuiSu Kim(김희수),Jin-Suk Lee(이진석),Sangmin Lee(이상민) 한국신재생에너지학회 2021 한국신재생에너지학회 학술대회논문집 Vol.2021 No.7
Polyhydroxybutyrate (PHB) is interested as a substitute for polypropylene. The effects of growth conditions on PHB production have been widely studied in chemolithotrophs, particularly in Rhodobacter. However, the results on PHB production in Rhodobacter have been inconstant due to different strains and experimental conditions, and it is unclear how diverse environmental factors are connected with PHB production. Here, we report optimized growth conditions for PHB production and show that the growth conditions are closely associated with reactive oxygen species (ROS) regulation. PHB accumulates in cells up to 50% at the highest level under dark aerobic conditions as opposed to light aerobic/anaerobic conditions. According to the time course, PHB contents increase at 48h and then gradually decreased. When observing the effect of temperature and medium composition on PHB production, 30℃ and C/N ratio of 9:1 or more were found to be most effective. Among PHB biosynthetic genes, PhaA and PhaB are highly correlated with PHB production, while PhaC and PhaZ observer little change in overall expression levels. We found that peroxidase activities and expression levels of antioxidant-related genes in cells under dark conditions were relatively high, whereas the amount of H<sub>2</sub>O<sub>2</sub> was comparatively low compared to the light conditions. These results suggest optimal culture conditions for growth and PHB production and the importance of ROS-scavenging signaling with regard to PHB production.
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