괴화 추출물이 대식세포에서의 nitric oxide와 interleukin-6의 생성에 미치는 영향

이지은,이주연,최점일,김종관,김성조,Lee, Ji-Eun,Lee, Ju-Youn,Choi, Jeom-Il,Kim, Chong-Kwan,Kim, Sung-Jo 대한치주과학회 2005 Journal of Periodontal & Implant Science Vol.35 No.1

Both nitric oxide (NO) and interleukin-6 (IL-6) have been thought to have a role in the pathogenesis of inflammatory periodontal disease as it does in other inflammatory diseases, and the inhibitors of NO and IL-6 production have been considered as potential anti-inflammatory agents. In this study, we evaluated methanol extract of Sophorae Flos for inhibition of NO and IL-6 production in Prevotella intermedia LPS-induced mouse macrophages RAW264.7 cells. Dried Sopharae Flos was sliced, and extracted with 100% methanol. LPS from p. intermedia ATCC 25611 was prepared by the standard hot phenol-water method. NO production was assayed by measuring the accumulation of nitrite in culture supematants and IL-6 was measured using mouse IL-6 ELISA kit. Western blot analysis of iNOS and analysis of reverse transcription (RT)-PCR products were carried out. The methanol extract of Sophorae Flos concentration-dependently reduced the production of NO and the expression of iNOS protein and mRNA in RAw264.7 cells treated with P. intermedia LPS. Sophorae Flos also suppressed IL-6 production and the expression of IL-6 mRNA in RAw264.7 cells stimulated by P. intermedia LPS. The inhibition of NO and IL-6 production by Sophorae Flos may be useful in the therapy of inflammatory diseases such as periodontitis. This hypothesis, however, remains to be tested.

Identification of immunological parameters associated with the alveolar bone level in periodontal patients

Park, Chang-Seo,Lee, Ju-Yeon,Kim, Sung-Jo,Choi, Jeom-Il Korean Academy of Periodontology 2010 Journal of Periodontal & Implant Science Vol.40 No.2

Purpose: The present study was performed to clarify the relationship between periodontal disease severity and selected immunological parameters consisting of serum IgG titer against periodontopathogenic bacteria, the expression of the helper T-cell cytokine by gingival mononuclear cells, and patients' immunoreactivity to cross-reactive heat shock protein (HSP) epitope peptide from P. gingivalis HSP60. Methods: Twenty-five patients with moderate periodontitis had their gingival connective tissue harvested of gingival mononuclear cells during an open flap debridement procedure and peripheral blood was drawn by venipuncture to collect serum. The mean level of interproximal alveolar bone was calculated to be used as an index for periodontal disease severity for a given patient. Each of selected immunologic parameters was subject to statistical management to seek their correlations with the severity of periodontal disease. Results: A significant correlation could not be identified between serum IgG titers against specific bacteria (Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, Actinobacillus actinomycetemcomitans, and Streptococcus mutans) and the severity of periodontal disease. Expression of interleukin (IL)-10 by gingival mononuclear cells was statistically significant in the group of patients who had higher levels of alveolar bone height. However, a similar correlation could not be demonstrated in cases for IL-4 or interferon-$\gamma$. Patients' serum reactivity to cross-reactive epitope peptide showed a significant correlation with the amount of alveolar bone. Conclusions: It was concluded that expression of IL-10 by gingival mononuclear cells and patients' sero-reactivity to the cross-reactive HSP peptide of P. gingivalis HSP60 were significantly correlated with alveolar bone height.

Symposium : New Trends in Periodontal Therapy: Optimizing Papillary Dimension for Esthetic Restoration-Multidisciplinary Perio-ortho-prosthodontic Approaches

( Jeom Il Choi ) 대한치주과학회 2010 대한치주과학회 학술대회자료집 Vol.2010 No.1

고정성 보철치료의 영속성을 높이기 위한 지대치의 적합성

최점일(JEOM-IL CHOI) 대한치과의사협회 2010 대한치과의사협회지 Vol.48 No.9

The success in periodontal-prosthetic therapy lies in patient satisfaction with subjective and customized treatment regimen that has been refined for individual needs. To fulfill these requirements, multiple predictors may have to be taken into consideration in the comprehensive treatment planning. Incorporation of complex factors that guarantee the evidence-based therapy would include, but not limited to, osseous morphology, biotype of gingival around abutment, bone morphology of interdental bone and those around furcation area, abutment or bridge mobility. The periodontal-restorative interface should be of mutually protective. Also strategic and esthetic value of abutment teeth to be restored should also be taken into consideration. Taken together, all these should work in concert to enhance the predictability and longevity of abutment teeth in periodontal-prosthetic therapy.

Prior Immunization with Fusobacterium Nucleatum Interferes with Opsonophagocytosis Function of Sera against Porphyromonas Gingivalis

Choi, Jeom-Il,Borrello, Melinda A.,Cutler, Christopher W.,Zauderer, Maurice The Korean Academy of Periodontoloy 2000 Journal of Periodontal & Implant Science Vol.30 No.1

본 연구는 Balb/c mice를 이용하여 Porphyromonas gingivalis 381(Pg)로 면역하기 전에 Fusobacterium nucleatum ATCC 10953(Fn)로 면역한 Group 1(N=10)과 Pg 로만 단독 면역을 시행한 Group 2(N=10)로부터 채취한 혈청의 Pg에 대한 식균능력을 비교하는 데 그 목적이 있다. 면역 후 혈청항체는 Pg에 대해 현저히 상승하였으나, 두 그룹간의 평균 항체역가는 통계적으로 차이가 없었다. 식균능력을 비교한 결과, Pg로만 단독 면역한 경우 식균능력이 Fn으로 먼저 면역한 Group 1의 경우보다 현저히 높았으며, 혈청항체역가와 식균지수와는 긴밀한 상관관계를 보였다. 결론적으로 치주세균의 사전 감염은 후속적인 세균감염에 대한 숙주 면역기능(식균능력)에 교란을 가져 올 수 있다.

IgG subclass-dependent Recognition of Porphyromonas Gingivalis Antigens in the Early-onset Periodontitis

Choi, Jeom-Il,Yoshimobu, Fuminobu,Schifferle, Robert E.,Okuda, Katsuji The Korean Academy of Periodontoloy 1999 Journal of Periodontal & Implant Science Vol.29 No.4

본 연구는 세 종류의 Porphyromonas gingivalis(Pg) antigen의 IgG subclass associated recognition을 평가하기 위해 수행했었다. 총 35명의 조기발병형치주질환자중, Pg381에 대한 IgG2항체의 증가를 보이는 5명이 급속진 행형 치주질환자, IgG4의 증가를 보이는 6명의 환자(국소유년형 치주질환자 2명과 급속진행 형 치주질환자 4명), IgG2+4의 증가를 보이는 2명의 급속진행형 치주질환자 그리고 IgG1+2+4의 증가를 보이는 8명의 환자(국소 유년형 치주질환자 2명과 급속진행형 치주질환자 6명)으로 구성된 21명의 환자를 dot immunoblot analysis를 위해 선택했다. 실험에 사용된 정제된 항원은 Pg381에서 추출한 43-kd fimbrilin protein과 lipoplysaccharide(LPS), Pg A7A1-28(ATCC 53977)에서 추출한 capsularpolysaccharide(CPS)였다. Immunoblotting pattern은 IgG4 antibody가 fimbrial antigen에 강력히 반응함을 보여주었다. Fimbriae에 잘 반응하는 몇몇의 IgG4 antibody역시 antigen에 대해 양성반응을 보였다. 대조적으로 IgG2는 CPS antigen을 일차적으로 인식했다. 전부는 아니지만 대부분의 경우, single이나 group화된 IgG subclass는 모두 LPS antigen을 인식하지 못했다. 같은 group에서 염색강도의 개인적인 차이는 증명되었다. 이런 결과는 조기발 병형 치주질환에서 Pg의 fimbriae와 CPS가 immunodominant antigen이 될 수 있음을 제시한다. 더욱이 IgG subclass antibody가 이런 Pg의 immunoglobulin antigen을 선택적으로 인식함을 알았고, 이는 조기발병형치주질환의 병리에 immunodominant antigen과 함께 IgG의 기능적인 역할을 고려해야 함을 제시한다.

The lgG subclass responses in the Phenotypic subsets of the early-onset periodontitis

Chio, Jeom-Il 부산대학교 유전공학연구소 1999 분자생물학 연구보 Vol.15 No.-

본 연구는 조기발병형 치주염의 서로 다른 4가지 표현형에 있어서 Porphyromonas gingivalis(Pg) 381과 Actinobacillus actinomycetemcomitans(Aa) Y4에 대한 상승된 IgG subclass의 양상을 평가하기 위해 시행하였다. Subform Ⅰ(distinctive localized juvenile periodontitis pattern)에서 3명, subform Ⅱ(post juvenile periodontitis pattern)에서 19명, subform Ⅲ (localized but rapidly progressing pattern)에서 15명, subform Ⅳ (distinctive rapidly progressing periodontitis pattern)에서 15명의 환자를 조사하여 Pg에 대한 total IgG level은 subform Ⅱ와 Ⅵ보다 subform Ⅰ과 Ⅲ에서 훨씬 높게 나타났다. IgG3 level이 subform Ⅰ와 Ⅵ사이에서 현저한 차이가 있다는 점을 제외하고는, 다른 IgG subclass level에서 subrorm사이에 아무런 차이가 없었다. Pg에 대한 IgG subclass는 single class 혹은 다양한 group에서 상승되어 나타났으며, IgG1+2+4가 가장 흔하게 발견되었고, 다음으로 IgG4 단독, IgG2 단독, IgG2+4, IgG2+3+4가 빈번히 상승되어 발견되는데, 특히 severe form(subform Ⅲ & Ⅳ)에서 그러했다. 뿐만 아니라, IgG level은 subform Ⅱ, Ⅲ, Ⅳ와 일치하여 점차적으로 증가하였고, 반면에 IgG1/IgG4 ratio는 그와 일치하여 감소되었다. 이러한 ratio의 감소는 단백질성의 오래된 항원의 과부하로 인해 immunoglobulin gene의 전환을 가능하게 한다는 것을 나타내고 있다. Aa에 대한 IgG2 level은 다른 유형보다 subformⅠ에서 상당히 높았다. Pg에 대한 IgG2 levels이 subformⅠ의 국소 부위에서 발생하는 disease activity와 밀접한 관련이 있으며, Aa의 경우에는 이러한 관련성이 나타나지 않았다. Pg에 대한 IgG2 level은 18-25세에서 훨씬 높은 동시에 26-35세에서는 감소했으며 결국 30대 후반에서는 더 높은 수치로 되돌아갔다. 이러한 결과는 Pg에 대한 IgG2 및 IgG responsiveness(single combined)가 EOP severe form의 발달에 중요하게 작용하며 IgG2 levels은 IgG/IgG4 ratio와 더불어 EOP의 localized type이 generalized type으로 계속 진행하는 것을 조절하는 역할을 하는 것으로 보인다는 것을 강하게 시사하였다.

과대 동요치아를 이용한 계속금관 가공의치의 임상적 연구

최점일(Jeom Il Choi),이재봉(Jae Bong Lee) 대한치과의사협회 1985 대한치과의사협회지 Vol.23 No.12

Immunoglobulin Allotypes and Immunoglobulin G Subclass Responses to Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in Early-Onset Periodontitis

CHOI, JEOM-IL,HA, MEE-HYE,KIM, JUN-HONG,KIM, SUNG-JO 부산대학교 유전공학연구소 1996 분자생물학 연구보 Vol.12 No.-

The present study was performed to estimate the observed frequencies of the immunoglobulin heavy-chain(Gm) and light-chain (Km) allotypes among patients with early-oneset periodontitis (EOP) and their effect on the IgG2 subclass responses against Actinobacillus actinomycetemcomitans Y4 and Porphyromaonas gingivalis 381, respectively. Sixty-nine EOP patients, including 11 with localized juvenile periodontitis (LJP), 19 who had LJP, 15 with LJP-rapidly progressing periodontitis (RPP), and 24 with RPP, were examined for the Gm and Km allotypes by a hemagglutination inhibition test. Levels of immunoglobulin G2 (IgG2) antibodies against the two organisms were determined by enzyme-linked immunosorbent assay. Fifty race-and age-matched, periodontally healthy subjects were also included as a control group. The observed frequencies of the Gm haplotype afnb and Km(1) were significantly higher in the RPP and LJP groups, respectively. The G2m(n) + group of those with RPP and the Km(1) + group of those with LJP had significantly higher levels of IgG2 antibodies to A. actinomycetemcomitans and P. gingivalis, respectively. The results indicate that linkage disequilibrium of the G2m(n) locus in RPP patients or the Km(1) locus in LJP patients may be associated with high IgG2 antibody responses to the respective bacteria. It was reasoned that the IgG2 antibody responses are associated with the immunoglobulin allotypes. The function of IgG2 antibodies in their reaction to different bacterial antigens may be interpreted as either protective or nonprotective in the two different types of EOP (i.e., LJP and RPP).

The road not taken: beyond our embarrassment and skepticism

Choi, Jeom-Il Korean Academy of Periodontology 2019 Journal of Periodontal & Implant Science Vol.49 No.3