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Je Hwan Kim(金濟桓),So Hyun Baek(白蘇鉉),Jeom Ho Ryu(柳點鎬) 한국육종학회 1997 한국육종학회지 Vol.29 No.2
This study was carried out to investigate the effects of growth regulators on the microspore surival rate, pollen dimorphism, microspore-originated calli formation and plant regeneration. The anthers of rice were cultured on the N₆ basal medium supplemented with the single or the combination of α-naphthalen acetic acid (NAA), 2, 4-dichlorophenoxy acetic acid(2, 4-D) and Kinetin. Microspore surival rate was not greatly different among growth regulators in 5 days of culture, but it was the highest on the medium with 1 mg/L NAA(81.9%), and the lowest on the medium with 4 mg/L Kinetin (40.3%) in 20 days of culture. Appearance rate of S pollen was low on the medium with 4 mg/L, Kinetin (24.2%), and high on the medium with 1 mg/L NAA (75.6%) Both of callus induction and plant regeneration were good on the medium supplement with NAA or 2, 4-D each, the combination of NAA with Kinetin, and 2, 4-D with Kinetin. The rate of induction was best on the medium with 1 mg/L 2, 4-D (55.1%). But the plant was not differentiated in calli induced from the medium with only Kinetin supplemented. The frequency of plant regeneration was excellent in calli induced from the medium with 2 mg/L NAA (13.5%)
Kang Soo Lee(李康壽),Sun Young Choi(崔善英),Jeom Ho Ryu(柳點鎬) 한국육종학회 1997 한국육종학회지 Vol.29 No.4
This study was conducted to find out the suitable NaCl concentration and time for investigating the varietal differences for salinity tolerance in barley. Seeds of 17 varieties were exposed to eight levels of NaCl concentration ranging from 0 to 350mM at 20℃. The average NaCl concentration that inhibited 50% germination of the viable seeds was 306mM and standard deviation was highest in 300mM NaCl. The relationship between the NaCl concentration that inhibited 50% germination of the viable seeds and the germination percentage in 300mM NaCl was correlated with high correlation coefficient, below 1% level of probability, and the germination percentage was correlated with the germination speed at 4th day after sowing in 300mM NaCl. The varieties used could be classified into three groups by the germination speed at 4th day after sowing in the 300mM NaCl. Nulssalbori, Hinssalbori and Olbori were classified into the tolerant group, Mudeungssalbori, Duwonchapssalbori and Youngsanbori, Saessalbori, Kinssalbori, Hinchalssalbori, Sacheon 6 bori and Tapgolbori, Saechalssalbori, Naehanssalbori were classified as the semi-susceptible group, and Songhakbori, Chalbori, Chunchubori and Kwanghwalssalbori were classified as the susceptible group.
Esterase 同位酵素, 蛋白質 및 形態的 特性에 의한 고구마 品種 分類
Deog Su Kim(金德洙),Sung Kun Oh(吳聖根),Moon Sup Chin(陣文燮),Jeom Ho Ryu(柳點鎬) 한국육종학회 1996 한국육종학회지 Vol.28 No.2
This study was conducted to utilize effectively similar varieties of sweet potato at the National Crops Experiment Station of Korea in 1994. Those were classified by esterase isozymes, proteins and some morphological characteristics. The results were as follows. The esterase isozymes in the leaves were similar in Kanto 95-1 and Kanto 95-2, Beniastma, Beniaka, Beniazuma and Benikomachi, Kintoki and Kintoki-2, Hongmi and Hongmi-2, High Starch and Hi-Starch. The esterase isozymes in the tuberous roots were similar in Beniastma and Beniaka, shiroshistma and Shiroshastma. The protein banding patterns in the tuberous roots were similar in Kanto and Kanto 95-1, Beniastma, Beniaka, Beniazuma and Benikomachi, Simon and Simon-2, High Starch and Hi-Starch. The morphological characteristics in the field were similar in Kanto 95 and Kanto 95-1, Beniastma, Beniaka, Beniazuma and Benikomachi, Simon and Simon-2, High Starch and Hi-Starch. Beniastma and Beniaka were appeared as the similar varieties because those had the same esterase isozymes in the leaves, tuberous roots and protein banding pattern in the tuberous roots, and some morphological characteristics in the field.
참깨(Sesamum indicum L.)의 葯培養에 의한 半數體 育成 : II.Haploidal Callus 誘起에 미치는 低溫處理,培地,培養條件 및 品種의 影響
柳點鎬,金濟桓,杜洪秀,權泰鎬 전북대학교 농업과학기술연구소 1993 農大論文集 Vol.24 No.1
This studies were conducted to provide the basic information about the duration and temperature of cold treatment, media, cultural condition, varieties and F1 on callus induction using the anther of sesame(Sesamum indicum L. and S. orientale L.). 1. Duration of cold treatment to induct the haploidal callus was the most effective for 2~44days in 10˚C both Danbaek and Wild type. 2. Murashge and Skoog's medium was more effective than any others in anther culture. 3. Continous dark culture was the most effective and combination of dark and light was less effective than continous dark culture. 4. Induction ratio of haploidal callus in Danbaek and Wild type was the highest than any others and that of F1 hybrid was less than dornor plant.
大豆에 있어서 生育初基의 斷水處理가 還元糖 및 α-amylase에 미치는 影響
柳點鎬,崔善英,李康壽 全北大學校 1988 論文集 Vol.30 No.-
In order to obtain the basic information for clarifying the mechanism of water stress, effects of water removal (15 days after emergence) on dry weight, chlorophyll and reducing sugar content, α-amylase activity and isozyme pattern were investigated in the early growth stage of soybean plant. The results are as follows. 1. Dry weight of shoot was increased in both the control and the treatment, but the degree of increase was remarkably lower in the treatment than that in the control. After 9 days of the treatment, it was decreased about 42% compared to the control. 2. Water content(%) and chlorophyll content in the leaves showed nearly unchanged in the control, but remarkable decrease in the treatment. After 9 days of the treatment, they came to 60% and to 4.5mg.g DW and decreased 26% and 42%, respectively, compared to the control. 3. Reducing sugar content and α-amylase activity in the leaves remained at almost constant level in the control, but showed almost linear increase in the treatment. After 9 days of the treatment, they came to 26mg/g DW and to 20 units, and reached about 2 and 1.6 times, respectively, compared to the control. 4. α-amylase isozymes showed only b (light stained) and c (dark stained) isozymes in the control and remained in color intensity during the period of investigation, while, in the treatment, a new a band was observed 3 days after the treatment, and a and b isozmes also became dark stained condition from 7 days after the treatment.
柳點鎬 전북대학교 농업과학기술연구소 1988 農大論文集 Vol.19 No.-
To obtain the basic information for shortening the bleeding period, mass propagation and the production of interspecific hybrids, the lotus (Nelumbo nucifera gasrtn) embryos at 20 days after (lowering were cultured in vitro on the Murashige and Skoog's ( MS) medium which contained the combinations of NAA (O,1,2 ppm) and Kinetin (0,1,2 ppm). The results are as follows. 1. Rooting rate, root number, root length and shoot growth of lotus embryo were remarkable on the basal MS medium without NAA and Kinetin, The higher the concentration of NAA and Kinetin, the more severe the inhibition of their growth sllowed. 2. On the basal MS medium under the light culture, the rooting rate at 20 and 30 days after culture we-re 66% and 100%, and shoot length at those times were 5.9cn and 10.3cn, respectively. While, under the dark culture, the rooting' rate at 20 and 30 days after culture were 16 % and 33%, and shoot length were 10.9 cm and 14.3cm, respectively. 3. The explants at 20 days after culture in vitro could be transplanted on the soil pot. 4. The Induction of callus was not observed at all 5. On the whole, light cultural condition was better than the dark one for the growth of embryo.