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        Expression of rpoS, ompA and hfq genes of Cronobacter sakazakii strain Yrt2a during stress and viable but nonculturable state

        Jameelah, Maryam,Dewanti-Hariyadi, Ratih,Nurjanah, Siti 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.3

        Cronobacter spp. in powdered infant formula has been etiologically linked to meningitis and necrotizing enterocolitis in certain groups of infants. This study aimed to determine whether C. sakazakii Yrt2a strain experiencing desiccation stress could enter viable but nonculturable (VBNC) state as well as to examine the expression of genes associated with stress and virulence during the above states. Stress and VBNC conditions were determined based on viability and culturability assays. Expression of genes related to stress (rpoS) and virulence (hfq and ompA) was evaluated by real-time PCR. The results showed that C. sakazakii Yrt2a entered VBNC 24 days post exposure to 2 h of desiccation treatment. The expression of rpoS, hfq and ompA genes was up-regulated during stress conditions, suggesting that Cronobacter successfully managed stress to maintain its culturability while maintaining its virulence. The expression of the target genes decreased at VBNC state but remained higher than that of a normal state. These findings reinforce the assumption that C. sakazakii undergoing VBNC state maintains its pathogenicity.

      • KCI등재

        Expression of rpoS, ompA and hfq genes of Cronobacter sakazakii strain Yrt2a during stress and viable but nonculturable state

        Maryam Jameelah,Ratih Dewanti-Hariyadi,Siti Nurjanah 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.3

        Cronobacter spp. in powdered infant formula has been etiologically linked to meningitis and necrotizing enterocolitis in certain groups of infants. This study aimed to determine whether C. sakazakii Yrt2a strain experiencing desiccation stress could enter viable but nonculturable (VBNC) state as well as to examine the expression of genes associated with stress and virulence during the above states. Stress and VBNC conditions were determined based on viability and culturability assays. Expression of genes related to stress (rpoS) and virulence (hfq and ompA) was evaluated by real-time PCR. The results showed that C. sakazakii Yrt2a entered VBNC 24 days post exposure to 2 h of desiccation treatment. The expression of rpoS, hfq and ompA genes was up-regulated during stress conditions, suggesting that Cronobacter successfully managed stress to maintain its culturability while maintaining its virulence. The expression of the target genes decreased at VBNC state but remained higher than that of a normal state. These findings reinforce the assumption that C. sakazakii undergoing VBNC state maintains its pathogenicity.

      • KCI등재

        Physicochemical and Thermal Properties of Lignocellulosic Fiber from Gigantochloa Scortechinii Bamboo: Effect of Steam Explosion Treatment

        Siti Atiqa Al Zahra Mat Darus,Mariyam Jameelah Ghazali,Che Husna Azhari,Rozli Zulkifli,Ahmad Adlie Shamsuri,Hanifi Sarac,Mohd Tamizi Mustafa 한국섬유공학회 2020 Fibers and polymers Vol.21 No.10

        Bamboo is an abundant natural resource in Asia and one of the high potential fibers used to reinforce polymercomposites. This article presents a comparative study on the physicochemical and thermal properties of bamboo fiber (BF)from Gigantochloa scortechinii for untreated BF, steam explosion (SE) treatment BF, and steam explosion followed by alkali(SE-alkali) treatment BF. The physicochemical and thermal properties of BF were determined using energy dispersive X-ray(EDX) spectroscopy and thermogravimetric analysis (TGA), while scanning electron microscopy (SEM) was used toexamine the surfaces morphologies. A Fourier transform infrared (FTIR) spectroscopy was utilized to detect the presence offunctional groups. TGA results showed that SE BF was significantly more thermally stable than the untreated BF and SEalkalitreatment BF. Major changes in chemical composition and surface morphology of the bamboo fibers indicated thathemicellulose and lignin were removed by SE-alkali treatment. In conclusion, the BF surface is physically and chemicallymodified by the SE-alkali treatment.

      • KCI등재

        The Mechanical Performance of Sugar Palm Fibres (Ijuk) Reinforced Phenolic Composites

        Bushra Rashid,Zulkiflle Leman,Mohammad Jawaid,Mariyam Jameelah Ghazali,Mohamad Ridzwan Ishak 한국정밀공학회 2016 International Journal of Precision Engineering and Vol.17 No.8

        Sugar palm fibres are one of the natural fibres which have many features and need further study to understand their properties. The aim of this work is to investigate the flexural, compressive and impact properties of sugar palm fibres reinforced phenolic composites. Sugar palm fibres were used as a filler (particle size 150 μm) and with loading of 0, 10, 20, 30, and 40 vol.%. The fibres were treated by sea water and then fabricated into composites by hot press technique. Flexural, compressive, and impact tests were carried out as per ASTM D790, ASTM D695-08a, and ASTM D256 standards, respectively. Scanning electron microscopy (SEM) was used to investigate the morphology and the interfacial bonding of the fibres-matrix in composites. The results show that the mechanical properties of the composites improve with the incorporation of fibres. The composite of 30 vol.% particle loading exhibit optimum values which are 32.23 MPa, 61.66 MPa, and 4.12 kJ/m2 for flexural, compressive, and impact strength, respectively. This was because good compatibility of fibre-matrix bonding. Consequently, sugar palm fibre is one of the prospective fibres and could be used as a potential resource to reinforcement polymer composite.

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