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- 저자 : Jürgen Polle (검색결과 3 건)
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Ana A. Ramos,Jürgen Polle,Duc Tran,John C. Cushman,EONSEON JIN,João C. Varela 한국조류학회I 2011 ALGAE Vol.26 No.1
The physiology of the unicellular green alga Dunaliella salina in response to abiotic stress has been studied for several decades. Early D. salina research focused on its remarkable salinity tolerance and ability, upon exposure to various abiotic stresses, to accumulate high concentrations of β-carotene and other carotenoid pigments valued highly as nutraceuticals. The simple life cycle and growth requirements of D. salina make this organism one of the large-scale commercially exploited microalgae for natural carotenoids. Recent advances in genomics and proteomics now allow investigation of abiotic stress responses at the molecular level. Detailed knowledge of isoprenoid biosynthesis mechanisms and the development of molecular tools and techniques for D. salina will allow the improvement of physiological characteristics of algal strains and the use of transgenic algae in bioreactors. Here we review D. salina isoprenoid and carotenoid biosynthesis regulation, and also the biotechnological and genetic transformation procedures developed for this alga that set the stage for its future use as a production system.
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Gene expression profiling of <i>Dunaliella</i> sp. acclimated to different salinities
Kim, Minjung,Park, Seunghye,Polle, Jü,rgen E.W.,Jin, EonSeon Blackwell Publishing Asia 2010 Phycological research Vol.58 No.1
<P>SUMMARY</P><P>To investigate which genes may be important for growth under extreme conditions such as very low or high salinities, a survey of the <I>Dunaliella</I> sp. transcriptome was performed with a cDNA microarray which had been generated previously representing 778 expressed sequence tags. The comparative microarray analysis indicated that 142 genes differed in expression levels by more than twofold in cells grown at extreme salinities (0.08 M and 4.5 M NaCl) when compared with cells grown at intermediate salinity (1.5 M NaCl). Of these genes, 28 had increased expression and 57 were suppressed in cells grown at low salinity. In cells grown at high salinity, 43 genes showed increased expression and 69 genes showed suppressed expression. However, we did observe a large overlap in the expression of extreme salinity-responsive genes based on Venn diagram analysis, which found 55 genes that responded to both of the two extreme salinity conditions. Further, we found that several genes had similar expression levels under low and high salinities, including some general stress response genes that were upregulated in both extreme salinity conditions. For confirmation of the validity of the cDNA microarray analysis, expression of several genes was independently confirmed by the use of gene-specific primers and real-time polymerase chain reaction. The present study is the first large-scale comparative survey of the transcriptome from the microalga <I>Dunaliella</I> sp. acclimated to extreme salinities, thus providing a platform for further functional investigation of differentially expressed genes in <I>Dunaliella</I>.</P>
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Jeon, Hancheol,Jeong, Jooyeon,Baek, Kwangryul,McKie-Krisberg, Zaid,Polle, Jü,rgen E.W.,Jin, EonSeon Elsevier 2016 Algal research Vol.19 No.-
<P><B>Abstract</B></P> <P> <I>Dunaliella salina</I> is a unicellular halophilic green alga, which can survive even in saturated brine solutions (up to 5.0M NaCl). In <I>D. salina</I>, the carbonic anhydrase (CA, EC 4.2.1.1) is essential for cells to acquire carbon and to cope with the low CO<SUB>2</SUB> environment under high salt conditions. For the first time, the present study describes the existence of eight genes coding for different types of <I>D. salina</I> CAs: five alpha-type (DsCAs) and three gamma-type (DsgCAs). Beta-type CAs appear to be lacking in <I>D. salina</I>, as they could not be found. Under either high salt or limited CO<SUB>2</SUB> condition, the CAs from <I>D. salina</I> showed different expression patterns, with phylogenetically close CAs exhibiting similar gene expression patterns. For the biological characterization of DsCA2b, which is a newly identified α-type CA, the enzyme was successfully produced as a soluble protein by truncating the membrane spanning regions at both ends (trDsCA2b). It was demonstrated that this truncated trDsCA2b version of the CA enzyme was active. Purified trDsCA2b clearly showed increased CA activity at increased NaCl concentrations of up to 3.0M. Based on in silico analysis and our predicted 3D structure of DsCA2b, we propose that this enzyme is localized in the plasma membrane of cells and active on the extracellular side. Our results are in agreement with the hypothesis that the DsCAs are essential enzymes for carbon acquisition mechanism under salt stress and CO<SUB>2</SUB> stress in <I>D. salina</I>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Eight novel carbonic anhydrases (DsCAs) were identified in <I>D. salina</I> CCAP 19/18. </LI> <LI> <I>DsCAs</I> were regulated differently under salt and CO<SUB>2</SUB> stresses. </LI> <LI> The salt enhanced activity of recombinant DsCA2b endorses salt tolerance of <I>D. salina</I>. </LI> <LI> DsCAs are predicted to play the key role in carbon acquisition in <I>D. salina</I>. </LI> </UL> </P>
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