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Prediction of Genetic Gain in Sweet Corn using Selection Indexes
Isadora Gonçalves da Silva,Renata Castoldi,Hamilton César de Oliveira Charlo,Mateus de Souza Miranda,Thaíssa Dias Cardoso Nunes,Luciene Lacerda Costa,Ernane Miranda Lemes 한국작물학회 2020 Journal of crop science and biotechnology Vol.23 No.2
The cultivation of sweet corn is expanding in Brazil, but there are serious constraints about the availability of commercial cultivars. The selection of superior sweet corn genotypes can be performed based on selection indexes based on plant agronomic characteristics. Thus, the objective of this work was to compare selection indexes to select sweet corn genotypes aiming greater productions. The experiment was conducted at the Vegetable Experimental Station of the Federal University of Uberlândia, Campus Monte Carmelo. Eighteen sweet corn accessions (F3 generation) were evaluated. The selection indexes applied to the agronomic characteristics of sweet corn were: direct and indirect, the sum of ranks, desired gains and ideotype indexes. The characteristics evaluated presented significant differences among genotypes, except for stem diameter, prolificacy and grain number per corncob. The coefficients of variation were below 30%, and the genetic parameters were satisfactory for most of the characteristics. The greatest gains with direct selection were in production (21.23%) and productivity of commercial sweet corn ear (15.19%), however, the indirect gains are unsatisfactory for sweet corn selection, and the sum of ranks index provided a balanced distribution of gains. The sweet corn genotypes L2P11, L2P37, P45, L2L5P3, and L5P18 presented a superior performance for the set of characters evaluated.
Machado Ricardo,da Silva Isadora,Comparin Daniel,de Mattos Bianca Araujo Marques,Alberton Luiz Rômulo,da Silva Neto Ulisses Xavier 대한치과보존학회 2021 Restorative Dentistry & Endodontics Vol.46 No.1
Objectives: The aim of this study was to compare smear layer removal by conventional application (CA), passive ultrasonic irrigation (PUI), EasyClean (EC), and XP-Endo Finisher (XPF), using 17% ethylenediaminetetraacetic acid (EDTA) after chemomechanical preparation, as evaluated with scanning electron microscopy (SEM). Materials and Methods: Forty-five single-rooted human mandibular premolars were selected for this study. After chemomechanical preparation, the teeth were randomly divided into 5 groups according to the protocol for smear layer removal, as follows: G1 (control): CA of distilled water; G2 (CA): CA of 17% EDTA; G3 (PUI): 17% EDTA activated by PUI; G4 (EC): 17% EDTA activated by EC; and G5 (XPF): 17% EDTA activated by XPF. SEM images (×1,000) were obtained from each root third and scored by 3 examiners. Data were evaluated using the Kruskal-Wallis and Dunn tests (p < 0.05). Results: In the apical third, there were no statistically significant differences among the groups (p > 0.05). In the cervical and middle thirds, the experimental groups performed better than the control group (p < 0.05); however, G2 presented better results than G3, G4, and G5 (p < 0.05), which showed no differences among one another (p > 0.05). Conclusions: No irrigation method was able to completely remove the smear layer, especially in the apical third. Using CA for the chelating solution performed better than any form of activation.
Balhaddad Abdulrahman A.,Garcia Isadora M.,Maktabi Haifa,Ibrahim Maria Salem,Alkhubaizi Qoot,Strassler Howard,Collares Fabrício M.,Melo Mary Anne S. 대한치과보존학회 2021 Restorative Dentistry & Endodontics Vol.46 No.4
Objectives This study aimed to evaluate the effect of improper positioning single-peak and multi-peak lights on color change, microhardness of bottom and top, and surface topography of bulk fill and incremental composites after artificial aging for 1 year. Materials and Methods Bulk fill and incremental composites were cured using multi-peak and single-peak light-emitting diode (LED) following 4 clinical conditions: (1) optimal condition (no angulation or tip displacement), (2) tip-displacement (2 mm), (3) slight tip angulation (α = 20°) and (4) moderate tip angulation (α = 35°). After 1-year of water aging, the specimens were analyzed for color changes (ΔE), Vickers hardness, surface topography (Ra, Rt, and Rv), and scanning electron microscopy. Results For samples cured by single-peak LED, the improper positioning significantly increases the color change compared to the optimal position regardless of the type of composite (p < 0.001). For multi-peak LED, the type of resin composite and the curing condition displayed a significant effect on ΔE (p < 0.001). For both LEDs, the Vickers hardness and bottom/top ratio of Vickers hardness were affected by the type of composite and the curing condition (p < 0.01). Conclusions The bulk fill composite presented greater resistance to wear, higher color stability, and better microhardness than the incremental composite when subjected to improper curing. The multi-peak LED improves curing under improper conditions compared to single-peak LED. Prevention of errors when curing composites requires the attention of all personnel involved in the patient's care once the clinical relevance of the appropriate polymerization reflects on reliable long-term outcomes.
Balhaddad Abdulrahman A.,Garcia Isadora,Collares Fabrício,Felix Cristopher M.,Ganesh Nisha,Alkabashi Qoot,Massei Ward,Strassler Howard,Melo Mary Anne 대한치과보존학회 2020 Restorative Dentistry & Endodontics Vol.45 No.4
Objectives: This study investigated the effects of physically damaged and resin-contaminated tips on radiant emittance, comparing them with new undamaged, non-contaminated tips using 3 pieces of spectrophotometric laboratory equipment. Materials and Methods: Nine tips with damage and/or resin contaminants from actual clinical situations were compared with a new tip without damage or contamination (control group). The radiant emittance was recorded using 3 spectrophotometric methods: a laboratory-grade thermopile, a laboratory-grade integrating sphere, and a portable light collector (checkMARC). Results: A significant difference between the laboratory-grade thermopile and the laboratory-grade integrating sphere was found when the radiant emittance values of the control or damaged/contaminated tips were investigated (p < 0.05), but both methods were comparable to checkMARC (p > 0.05). Regardless of the method used to quantify the light output, the mean radiant emittance values of the damaged/contaminated tips were significantly lower than those of the control (p < 0.05). The beam profile of the damaged/contaminated tips was less homogeneous than that of the control. Conclusions: Damaged/contaminated tips can reduce the radiant emittance output and the homogeneity of the beam, which may affect the energy delivered to composite restorations. The checkMARC spectrophotometer device can be used in dental offices, as it provided values close to those produced by a laboratory-grade integrated sphere spectrophotometer. Dentists should assess the radiant emittance of their light-curing units to ensure optimal curing in photoactivated, resin-based materials.
Coaguila-Llerena Hernán,Barbieri Isadora,Tanomaru-Filho Mário,Leonardo Renato de Toledo,Ramos Ana Paula,Faria Gisele 대한치과보존학회 2020 Restorative Dentistry & Endodontics Vol.45 No.4
Objectives: The aim of this study was to assess the physicochemical properties, cytotoxicity and penetration into dentinal tubules of ChlorCid™ Surf (3% sodium hypochlorite [NaOCl] with surfactant) in comparison to ChlorCid™ (3% NaOCl without surfactant). Materials and Methods: The physicochemical properties evaluated were pH, surface tension, free available chlorine (FAC) and contact angle. Cytotoxicity was evaluated in L929 fibroblasts exposed to the solutions by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide and neutral red assays. Assessment of penetration into dentinal tubules was performed by staining single-rooted permanent human teeth with crystal violet (n = 9), which were irrigated with the solutions and analyzed in cervical, middle and apical segments. Data were analyzed by one-way analysis of variance (ANOVA) and Tukey's post-test, 2-way ANOVA and Bonferroni's post-test or t-test (α = 0.05). Results: ChlorCid™ Surf and ChlorCid™ FAC values were close to those indicated by the manufacturer. ChlorCid™ Surf showed lower surface tension and contact angle on dentin, and higher pH than ChlorCid™ (p < 0.05). The penetration of ChlorCid™ Surf was higher in cervical and middle segments, compared with ChlorCid™ (p < 0.05). There was no difference in irrigant cytotoxicity (p > 0.05). Conclusions: ChlorCid™ Surf showed lower surface tension, lower contact angle on root canal dentin, higher penetration into dentinal tubules and more alkaline pH, compared with ChlorCid™. However, both solutions showed similar cytotoxicity and FAC content.
Sérgio Lúcio Pereira de Castro Lopes,Isadora Luana Flores,Thiago de Oliveira Gamba,Rivea Ines Ferreira-Santos,Mari Eli Leonelli de Moraes,Aline Alvarez Cabello,Paula Nascimento Moutinho 대한구강악안면외과학회 2017 대한구강악안면외과학회지 Vol.43 No.2
Maxillofacial images must be examined to find pathologies not identified during clinical examination. Unicystic ameloblastoma (UA) extending to the mandibular body and ramus was neglected on initial panoramic radiographic examination. After orthodontic therapy, a huge lesion was observed clinically and through imaging exams. After the conservative surgery, no recurrence was observed during five years of follow-up. This case emphasized the need for careful evaluation of patient images focusing on the oral diagnosis before any dental treatment planning, including orthodontic therapy.
Sandra Branco,João Orvalho,Alexandre Leitão,Isadora Pereira,Manuel Malta,Isabel Mariano,Tânia Carvalho,Rui Baptista,Brian R. Shiels,Maria C. Peleteiro 대한수의학회 2010 JOURNAL OF VETERINARY SCIENCE Vol.11 No.1
This study was carried out to investigate fifteen cases of acute lethal infection of calves (≤ 4 months of age) by the protozoan parasite Theileria (T.) annulata in the south of Portugal. Calves developed multifocal to coalescent nodular skin lesions, similar to multicentric malignant lymphoma. Infestation with ticks (genus Hyalomma) was intense. Theileria was seen in blood and lymph node smears, and T. annulata infection was confirmed by isolation of schizont-transformed cells and sequencing of hypervariable region 4 of the 18S rRNA gene. At necropsy, hemorrhagic nodules or nodules with a hemorrhagic halo were seen, particularly in the skin, subcutaneous tissue, skeletal and cardiac muscles, pharynx, trachea and intestinal serosa. Histologically, nodules were formed by large, round, lymphoblastoid neoplastic-like cells. Immunohistochemistry (IHC) identified these cells as mostly CD3 positive T lymphocytes and MAC387 positive macrophages. A marker for B lymphocytes (CD79αcy) labeled very few cells. T. annulata infected cells in these nodules were also identified by IHC through the use of two monoclonal antibodies (1C7 and 1C12) which are diagnostic for the parasite. It was concluded that the pathological changes observed in the different organs and tissues were caused by proliferation of schizont-infected macrophages, which subsequently stimulate a severe uncontrolled proliferation of uninfected T lymphocytes.
Bohns Fábio Rocha,Leitune Vicente Castelo Branco,Garcia Isadora Martini,Genari Bruna,Dornelles Nélio Bairros,Guterres Silvia Stanisçuaski,Ogliari Fabrício Aulo,de Melo Mary Anne Sampaio,Collares Fabrí 대한치과보존학회 2020 Restorative Dentistry & Endodontics Vol.45 No.4
Objectives: In this study, we investigated the potential of amoxicillin-loaded polymeric microspheres to be delivered to tooth root infection sites via a bioactive reparative cement. Materials and Methods: Amoxicillin-loaded microspheres were synthesized by a spray-dray method and incorporated at 2.5% and 5% into a mineral trioxide aggregate cement clinically used to induce a mineralized barrier at the root tip of young permanent teeth with incomplete root development and necrotic pulp. The formulations were modified in liquid:powder ratios and in composition by the microspheres. The optimized formulations were evaluated in vitro for physical and mechanical eligibility. The morphology of microspheres was observed under scanning electron microscopy. Results: The optimized cement formulation containing microspheres at 5% exhibited a delayed-release response and maintained its fundamental functional properties. When mixed with amoxicillin-loaded microspheres, the setting times of both test materials significantly increased. The diametral tensile strength of cement containing microspheres at 5% was similar to control. However, phytic acid had no effect on this outcome (p > 0.05). When mixed with modified liquid:powder ratio, the setting time was significantly longer than that original liquid:powder ratio (p < 0.05). Conclusions: Lack of optimal concentrations of antibiotics at anatomical sites of the dental tissues is a hallmark of recurrent endodontic infections. Therefore, targeting the controlled release of broad-spectrum antibiotics may improve the therapeutic outcomes of current treatments. Overall, these results indicate that the carry of amoxicillin by microspheres could provide an alternative strategy for the local delivery of antibiotics for the management of tooth infections.
Marconi Daniel Feijolo,da Silva Giovana Siocheta,Weissheimer Theodoro,Silva Isadora Ames,Só Gabriel Barcelos,Jahnke Leonardo Thomasi,Skupien Jovito Adiel,Só Marcus Vinicius Reis,da Rosa Ricardo Abreu 대한치과보존학회 2022 Restorative Dentistry & Endodontics Vol.47 No.4
Objectives This study aimed to investigate the influence of different obturation techniques compared to cold lateral compaction on the success rate of primary non-surgical endodontic treatments. Materials and Methods Systematic searches were performed for studies published up to May 17th, 2022 in MEDLINE/PubMed, Cochrane Library, Web of Science, Scopus, EMBASE, and Grey Literature Reports. Randomized clinical trials and nonrandomized (nonrandomized clinical trials, prospective or retrospective) studies that evaluated the success rate of primary non-surgical endodontic treatments obturated with the cold lateral compaction (control) and other obturation techniques were included. The revised Cochrane risk of bias tools for randomized trials (RoB 2) and nonrandomized studies of interventions (ROBINS-I) were used to evaluate the risk of bias. The Grading of Recommendations Assessment, Development, and Evaluation (GRADE) tool was used to evaluate the certainty of evidence. Results Eleven studies (4 randomized clinical trials (RCTs), 4 prospective, and 3 retrospectives) were included. Two RCTs were classified as having some concerns risk of bias and 2 as a low risk of bias. Two nonrandomized studies were classified as having a critical risk of bias and 5 as having a moderate risk of bias. The GRADE analysis demonstrated a very low to moderate certainty of evidence. Conclusions This systematic review generally evidenced no differences in the success rate of primary non-surgical endodontic treatments when the cold lateral compaction technique and other obturation techniques are performed. Further well-designed studies are still necessary.