Micropatterning proteins on polyhydroxyalkanoate substrates by using the substrate binding domain as a fusion partner

Park, Jong Pil,Lee, Kyung-Bok,Lee, Seok Jae,Park, Tae Jung,Kim, Min Gon,Chung, Bong Hyun,Lee, Zee-Won,Choi, Insung S.,Lee, Sang Yup John Wiley & Sons 2005 Biotechnology and Bioengineering Vol.92 No.2

<P>A novel strategy for micropatterning proteins on the surface of polyhydroxyalkanoate (PHA) biopolymer by microcontact printing (µCP) is described. The substrate binding domain (SBD) of the Pseudomonas stutzeri PHA depolymerase was used as a fusion partner for specifically immobilizing proteins on PHA substrate. Enhanced green fluorescent protein (EGFP) and red fluorescent protein (RFP) fused to the SBD could be specifically immobilized on the micropatterns of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate). Laser scanning confocal microscopic studies suggested that two fusion proteins were micropatterned in their functionally active forms. Also, antibody binding assay by surface plasmon resonance suggested that protein–protein interaction studies could be carried out using this system. © 2005 Wiley Periodicals, Inc.</P>

Preliminary Study on Multiple Oxygen Isotopic Composition of Sulfate in the Fresh Water, King Sejong Station, King George Island, Antarctica

Insung Lee,Jong Ik Lee,Byong-Kwon Park,Huiming Bao,Alan J. Kaufman,Sang Bum Park,Guebuem Kim 대한지질학회 2008 대한지질학회 학술대회 Vol.2008 No.

Stability Phase Diagram of GdBa2Cu3O7-δ for the Nominal Composition of Gd:Ba:Cu = 1:1:2.5 in Low Oxygen Pressures

Park, Insung,Oh, Won-Jae,Lee, Jae-Hun,Moon, Seung-Hyun,Yoo, Sang-Im Institute of Electrical and Electronics Engineers 2018 IEEE transactions on applied superconductivity Vol.28 No.4

<P>The phase stability of the GdBa<SUB>2</SUB>Cu<SUB>3</SUB>O<SUB>7-δ</SUB> (GdBCO) compound and stable phases were carefully investigated for the nominal composition of Gd:Ba:Cu = 1:1:2.5 in low oxygen pressures (<I> P</I>O<SUB>2</SUB>) of 1–150 mTorr. For this study, Gd-Ba-Cu-O amorphous films were deposited on LaAlO <SUB>3</SUB> (001) substrates at 200  °C by pulsed laser deposition and used as precursor films. Using a reel-to-reel tube furnace, as-deposited precursor films were annealed at various high temperatures in low <I>P </I>O<SUB>2</SUB> atmospheres and then rapidly cooled down to room temperature. As a result, the stability boundary of GdBCO for this composition was found to be remarkably shifted to the lower temperature regions compared with that of the nominal composition for Gd:Ba:Cu = 1:2:3. Also, it was found for this composition that with increasing temperature at a given <I>P</I>O<SUB>2</SUB>, three-phase equilibrium among Gd123, Gd<SUB>2</SUB> CuO<SUB>4</SUB>, and Cu<SUB>2</SUB>O below the GdBCO phase boundary first changed into two-phase equilibrium between Gd <SUB>2</SUB>CuO<SUB>4</SUB> and liquid, and then changed into another two-phase equilibrium between Gd<SUB>2</SUB>O<SUB> 3</SUB> and liquid.</P>

Salt-Induced, Continuous Deposition of Supramolecular Iron(III)-Tannic Acid Complex

Park, Taegyun,Kim, Won Il,Kim, Beom Jin,Lee, Hojae,Choi, Insung S.,Park, Ji Hun,Cho, Woo Kyung American Chemical Society 2018 Langmuir Vol.34 No.41

<P>One-step assembly of iron(III)-tannic acid (Fe<SUP>3+</SUP>-TA) complex forms nanothin (∼10 nm) films on various substrates within minutes. In this deposition scheme, however, the film does not grow continuously over time even though Fe<SUP>3+</SUP>-TA complex is still abundant in the coating solution. In this paper, we report that the salt addition dramatically changes the one-off coating characteristic to continuous one, and each salt has its optimum concentration (<I>C</I><SUB>MFT</SUB>) that produces maximum film thickness. For detailed investigation of the salt effects, we employed various salts, including LiCl, NaCl, KCl, CaCl<SUB>2</SUB>, SrCl<SUB>2</SUB>, BaCl<SUB>2</SUB>, NaBr, and NaNO<SUB>3</SUB>, and found that only cations played an important role in the continuous deposition of the Fe<SUP>3+</SUP>-TA complex, with smaller <I>C</I><SUB>MFT</SUB> values for the cations of higher valency and larger size. On the basis of the results, we suggested that the positively charged cations screened the negative surface charges of Fe<SUP>3+</SUP>-TA complex particles, leading to coagulation and continuous deposition, further supported by the ζ-potential measurement and time-resolved dynamic light-scattering analysis.</P> [FIG OMISSION]</BR>

Effects of Body Pillow Use on Sleeping Posture and Sleep Architecture in Healthy Young Adults

Park Insung,Suzuki Chihiro,Suzuki Yoko,Kawana Fusae,Yajima Katsuhiko,Fukusumi Shoji,Kokubo Toshio,Tokuyama Kumpei,Yanagisawa Masashi,Satoh Makoto 대한수면학회 2021 sleep medicine research Vol.12 No.1

Background and Objective Patients with position-dependent obstructive sleep apnea have a > 2-fold higher apnea-hypopnea index when sleeping in a supine position compared with a non-supine position. We investigated the effect of body pillow use on sleeping body position and sleep architecture in healthy young adults. Methods In experiment 1, we evaluated the body pressure distribution with or without body pillow use in 8 healthy young adults [age, 36.5 ± 13.0 years; body mass index (BMI); 20.6 ± 1.2 kg/m2]. In experiment 2, we performed a randomized-crossover intervention study to evaluate the effects of body pillow use on sleeping position and sleep architecture in 10 healthy young adults (age, 24.3 ± 7.8 years; BMI, 21.4 ± 1.7 kg/m2). Sleep architecture was characterized by polysomnography, and body positions were monitored using a sensor. Subjective sleep quality was evaluated with the Oguri-Shirakawa-Azumi sleep inventory, middle age and aged version. Results In experiment 1, body pillow use significantly reduced mean body pressure on the shoulder, hip, and whole body. In experiment 2, mean time spent in the supine, lateral, and prone body positions did not differ significantly between the 2 trials. Body pillow use, however, significantly extended the sustained time spent in the lateral body position compared with the control trial. Subjective sleep quality and sleep architecture did not differ significantly between the 2 trials, but body pillow use decreased the number of short (30 s) slow-wave sleep episodes. Conclusions Sleeping with a body pillow effectively extends sustained time in a lateral sleeping position and prevents segmentation of slow-wave sleep episodes.

The fabrication of bulk magnet stacked with HTS tapes for the magnetic levitation

Park, Insung,Kim, Gwantae,Kim, Kyeongdeok,Sim, Kideok,Ha, Hongsoo 한국초전도저온학회 2022 한국초전도저온공학회논문지 Vol.24 No.3

With the innovative development of bio, pharmaceutical, and semiconductor technologies, it is essential to demand a next-generation transfer system that minimizes dust and vibrations generated during the manufacturing process. In order to develop dust-free and non-contact transfer systems, the high temperature superconductor (HTS) bulks have been applied as a magnet for levitation. However, sintered HTS bulk magnets are limited in their applications due to their relatively low critical current density (J_c) of several kA/cm² and low mechanical properties as a ceramic material. In addition, during cooling to cryogenic temperatures repeatedly, cracks and damage may occur by thermal shock. On the other hand, the bulk magnets made by stacked HTS tapes have various advantages, such as relatively high mechanical properties by alternate stacking of the metal and ceramic layer, high magnetic levitation performance by using coated conductors with high J_c of several MA/cm², consistent superconducting properties, miniaturization, light-weight, etc. In this study, we tried to fabricate HTS tapes stacked bulk magnets with 60 mm × 60 mm area and various numbers of HTS tape stacked layers for magnetic levitation. In order to examine the levitation forces of bulk magnets stacked with HTS tapes from 1 to 16 layers, specialized force measurement apparatus was made and adapted to measure the levitation force. By increasing the number of HTS tapes stacked layers, the levitation force of bulk magnet become larger. 16 HTS tapes stacked bulk magnets show promising levitation force of about 23.5 N, 6.538 kPa at 10 mm of levitated distance from NdFeB permanent magnet.

Structure Modulation of Silica Microspheres in Bio-Inspired Silicification: Effects of TEOS Concentration

Park, Ji Hun,Choi, Ji Yu,Park, Taegyun,Yang, Sung Ho,Kwon, Sunbum,Lee, Hee-Seung,Choi, Insung S. Wiley Blackwell (John Wiley Sons) 2011 Chemistry, an Asian journal Vol.6 No.8

Hardware-Accelerated Multipipe Parallel Rendering of Large Data Streams

Park, Sanghun,Park, Sangmin,Bajaj, Chandrajit,Ihm, Insung Korea Computer Graphics Society 2001 컴퓨터그래픽스학회논문지 Vol.7 No.2

최근 과학적 데이터의 증가 결과로, 매우 방대한 크기의 볼륨 데이터들이 점차로 일반화되고 있다. 다양한 텍스춰 기반 볼륨 렌더링 알고리듬들이 제안되었으나, 대부분의 방법들이 하드웨어가 갖고 있는 텍스춰 메모리보다 작은 크기의 볼륨 데이터를 가시화하는데 촛점을 맞추고 있다. 본 논문은 멀티파이프 시스템 구조상에서 매우 큰 정적 데이터와 시간 가변 데이터를 위한 새로운 병렬 볼륨 렌더링 스킴에 대해 설명한다. 이 스킴은 큰 볼륨을 동적으로 작은 크기의 블릭으로 분할하고, 이를 그래픽스 파이프에 적절히 할당함으로써 텍스춰 교체 비용을 최소화 한다. 이 기법은, 수행중에 법선 벡터를 계산하고 OpenGL 컬러 행렬을 이용하여 퐁 쉐이딩 영상을 쉽게 생성할 수 있다. 우리는 SGI Onyx2 시스템을 이용하여 큰 크기의 다양한 데이터에 대해 실험한 결과를 보인다. As a result of the recent explosive growth of scientific data, extremely large volume datasets have become increasingly commonplace. While several texture-based volume rendering algorithms have been proposed, most of them focused on volumes smaller than the hardware's available texture memory. This paper presents a new parallel volume rendering scheme for very large static and time-varying data on a multipipe system architecture. Our scheme subdivides large volumes dynamically into smaller bricks, and assigns them adaptively to graphics pipes to minimize the costs of texture swapping. With the new method, Phong shaded images can be easily created by computing the gradients on the fly and using the color matrix feature of OpenGL. We report experimental results on an SGI Onyx2 for the various large datasets.

전도성 올리고전해질과 에리스로신 B간 형광공명 에너지전달에 의한 수용액 기반 광전류 생성

강인성(Insung Kang),박종협(Jonghyup Park),조현진(Hyunjin Jo),박주현(Juhyun Park) 한국고분자학회 2015 폴리머 Vol.39 No.2

수용액 기반 광전류 생성 연구는 자연의 광합성을 모사하여 친환경적으로 전기를 생산하기 위한 연구의 하나로서 주목받고 있다. 본 연구에서는 지질 소액포에 집적된 전도성 올리고전해질과 수용액 중에 용해된 형광염료를 이용하여 광전류 생성 소자를 제작하고, 두 염료 사이의 형광공명 에너지전달 현상이 결과적인 광전류 생성에 미치는 영향에 대해 조사하였다. 양극성 전도성 올리고전해질은 지질 이중층 내에 집적되어 지질 소액포의 형태로 수용액 중에 분산되었고 이 수용액에 형광염료를 농도를 변화시키며 형광공명 에너지전달 효율과 광전류의 변화를 측정하였다. 전자공여체인 전도성 올리고전해질에서 전자수용체인 형광염료로 에너지전달 효율은 형광염료 농도의 증가에 따라 증가하였으며, 광전류 생성은 증가 후 감소하였다. 광전류 변화에 있어서 염료 사이의 에너지전달과 전자수송체의 역할에 대해 논의하였다. Aqueous-based, environmentally friendly photocurrent generation has been highlighted to produce electricity by mimicking photosynthesis in nature. We fabricated a photocurrent generation system using a conjugated oligoelectrolyte (COE) assembled in lipid vesicles and a fluorescence dye, and investigated the fluorescence resonance energy transfer (FRET) between two species and the influence of FRET on the photocurrent generation. The FRET efficiency from the electron donor, COE, to the electron acceptor, the dye, increased with the dye concentrations, but the photocurrent increased and then decreased with the dye concentrations. We discussed about the role of FRET and electron shuttles over the variation of photocurrent.

Organic ferroelectric field-effect transistor with P(VDF-TrFE)/PMMA blend thin films for non-volatile memory applications

Bae, Insung,Kang, Seok Ju,Park, Youn Jung,Furukawa, T.,Park, Cheolmin Elsevier 2010 Current Applied Physics Vol.10 No.1

<P>Among the many ferroelectrics polymers, poly(vinylidene fluoride-co-trifluoroethylene) [P(VDF-TrFE)] has drawn a great attention with its promising memory properties such as large remanent polarization, good fatigue and retention properties. Since the ferroelectric layer plays a critical role in the operation of memory device, it is important to control the structure of ferroelectric thin film. In order to improve the performance of a ferroelectric device, in this contribution we employed P(VDF-TrFE) layers blended with various compositions of an amorphous poly(methyl methacrylate) (PMMA) from 0 wt.% to 20 wt.%. In metal/ferroelectric/metal capacitor structure, we observed the decrease of remanent polarization from 9.13 mu C/cm(2) to 4.7 mu C/cm(2) and increase of coercive voltage from 9.5 V to 15.2 V with PMMA. Polarization switching time of the ferroelectric blend films estimated from the switched polarization vs. time curves increases with the amount of PMMA. Furthermore, ferroelectric field-effect transistors (FeFETs) based on ferroelectric P(VDF-TrFE) and PMMA blend films with single-crystalline tri-isopropylsilylethynyl pentacene (TIPS-PEN) channels show that both ON and OFF currents of the transistors are maintained with the PMMA contents despite the reduction of remanent polarization of P(VDF-TrFE)/PMMA films. (C) 2009 Elsevier B.V. All rights reserved.</P>