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      • KCI등재

        Development of a Microbial Consortium for Dairy Wastewater Treatment

        C. A. Mazzucotelli,I. Durruty,C. E. Kotlar,M. R. Moreira,A. G. Ponce,S. I. Roura 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.2

        The wastewater from the dairy industries usuallycontains high concentrations of contaminants and, since thevolume generated is also high, the total contaminant load isvery significant. Among the available options for treatment,biological degradation looks like the most promising one. Furthermore, the supplementation of the native microbialpopulations with external microorganisms with high specificdegradation rates (bio-augmentation) has demonstrated toimprove the performance of treatment. The main objectiveof this research was to select a combination of bacteria toimprove the aerobic treatment of dairy processingwastewater. For this purpose, eleven fat/protein-degradingmicroorganisms belonging to the genera Bacillus, Serratia,Lactococcus, Enterococcus, Stenotrophomonas, Klebsiellaand Escherichia, were evaluated as potential degradingbacteria using a Plackett-Burman design. Assays werecarried out to select the strains that most significantlyinfluenced the degradation of wastewater and biomassyield, in terms of COD removal. A simulated dairyindustry effluent was used as culture medium. Four strainswere selected as potential members of the microbialconsortium: Lactococcus garvieae, Bacillus thuringiensis,Escherichia coli and Stenotrophomonas sp. The optimaloperation temperature and pH range of the selectedconsortium were 32°C and 6 ~ 8, respectively. Thedegradation percentages reached with the selected consortiumwere 80.67 and 83.44% at 24 and 48 h, respectively. Theselected consortium significantly improved the degradationof the dairy wastewater, and the degradation degreeachieved by this consortium was higher than by using thestrains individually.

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