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Cholong KIM,Hyeyeon HUR,Seungju OH,Jongmin YANG,Seongchul SHIN,Gillhwan KIM,Dhanashri DESAI,Hyunho LEE 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Today, waste plastics after humans usage frequently causes serious environmental problems and their detection methods have been lagged due to many obstacles. In this study, using polystyrene binding peptide (PSBP), which was combined into micropatterns having gold nanorods (Au NRs), microplastic detection system was developed from lithographic trimethylolpropane ethoxylate triacrylate (ETPTA) micropatterning. Au NRs could amplify the peak of polystyrene (PS) microplastics under FT-IR spectroscopy, which could make PS detection more efficiently. Its effect is believed to be a plasmonic origin and it could detect microplastics in low PS concentration to solve problem associated with vibrational spectroscopic detection.
Microfluidics Mixer Platform for Efficient Fluorescent Labeling of Microplastics
Seongcheol SHIN,Hyun ho LEE,Seungju OH,Hyeyeon HUR,Jongmin YANG,Dhanashri DESAI,Gillhwan KIM,Chorong KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Microplastics are small plastic particles less than 5 mm, and their environmental problems are caused by their increased accumulations throughout the food chains. However, there have been a number of hardships to qualify and quantify the amount of microplastics. In this study, we fabricate microfluidic mixer device using polydimethylsiloxane (PDMS) by SU-8 photolithography or by laser ablation method for efficient fluorescent labeling of individual microplastics inside of serpentine microfluidic channel. It shows that mixing channel is an efficient platform to fluorescently label the microplastics with a short time scale additionally with recirculation of fluorescent labeling mixture.
Microfluidic Microplastics Trap Module for Detection by Fluorescent Staining
Seongcheol SHIN,Hyun Ho LEE,Seungju OH,Hyeyeon HUR,Jongmin YANG,Dhanashiri DESAI,Gillhwan KIM,Chorong KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Microplastics has a negative impact on marine ecosystems and humans. In this study, we developed microfluidic devices as microplastics trap modules using polydimethylsiloxane (PDMS) using photolithography or laser ablation. For a generation of turbulent flow to separate microplastics, several grooves were pattered inside the microfluidic channel. It proves that grooved channel is an efficient platform to trap the microplastics by recirculation. Finally, the microfluid trap module could be connected with PDMS mixer module to selectively stain the trapped microplastics my fluorescent dye.