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      • KCI등재

        中国的挂牌公司制度

        石慧?(Shi Huirong) 한국법학회 2014 법학연구 Vol.55 No.-

        상장회사는 다원화 자본시장의 구축과정에 따라 나타나는 새로운 회사형태이다. 상장회사의 주식은 전국 주식양도 시스템을 통해 진행하는 거래이다. 청구주식은 전국 주식 양도시스템에서 상장하고 주식 소유제도에 대한 제한되지 않고, 고급 신기술 기업만 나오는 것이 아니다. 상장 주식의 양도방식은 협의방식, 조시방식, 경갑방식 또는 중국 증권감독위원회에 허락하는 양도방식으로 채택할 수 있다. 상장회사는 상장 조건을 만족하면 바로 증권거래소에 상장신청할 수 있다.

      • KCI등재

        中国的挂牌公司制度

        石 慧 荣 (Shi Huirong) 한국법학회 2014 법학연구 Vol.55 No.-

        挂牌公司是随着多层次资本市场的构建进程而出现的一种新型公司样态。挂牌公司的的股票可通过全国股份转让系统进行交易。申请股票在全国股份转让系统挂牌,不受股东所有制性质的限制,不限于高新技术企业。挂牌股票的转让可以采取协议方式、做市方式、竞价方式或其他中国证监会批准的转让方式。挂牌公司达到上市条件的,可以直接向证券交易所申请上市。

      • Preventing Cooperative Black Hole Attacks in Mobile Ad Hoc Networks: Simulation Implementation and Evaluation

        Hesiri Weerasinghe,Huirong Fu 보안공학연구지원센터 2008 International Journal of Software Engineering and Vol.2 No.3

        A black hole attack is a severe attack that can be easily employed against routing in mobile ad hoc networks. A black hole is a malicious node that falsely replies for any route requests without having active route to specified destination and drops all the receiving packets. If these malicious nodes work together as a group then the damage will be very serious. This type of attack is called cooperative black hole attack. In [9], we proposed a solution to identifying and preventing the cooperative black hole attack. Our solution discovers the secure route between source and destination by identifying and isolating cooperative black hole nodes. In this paper, via simulation, we evaluate the proposed solution and compare it with other existing solutions in terms of throughput, packet loss percentage, average end-to-end delay and route request overhead. The experiments show that (1) the AODV greatly suffers from cooperative black holes in terms of throughput and packet losses, and (2) our solution proposed in [9] presents good performance in terms of better throughput rate and minimum packet loss percentage over other solutions, and (3) our solution proposed in [9] can accurately prevent the cooperative black hole attacks.

      • Beacon-less Location Detection in Wireless Sensor Networks for Non-flat Terrains

        Sireesha Krupadanam,Huirong Fu 보안공학연구지원센터 2008 International Journal of Software Engineering and Vol.2 No.3

        In this paper, we develop a new beacon-less localization scheme for non-flat surfaces for wireless sensor networks. Simulations are conducted for a Gaussian distribution of nodes about the deployment points on the non-flat terrain, as well as a node deployment corresponding to the terrain surface characteristics. The localization errors for the proposed method, for a non-flat terrain with the Gaussian distribution of nodes and the terrain conforming distribution, are comparable to those in the case of the location discovery scheme in [1] for the flat surface. The localization errors, when the distribution of nodes corresponds to the terrain are slightly larger than when the nodes are normally distributed. The results are applicable to localization in real life wireless sensor networks deployed over non-flat terrains.

      • SCIESCOPUSKCI등재

        Spatial Pattern of Copper Phosphate Precipitation Involves in Copper Accumulation and Resistance of Unsaturated Pseudomonas putida CZ1 Biofilm<sup>s</sup>

        ( Guangcun Chen ),( Huirong Lin ),( Xincai Chen ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.12

        Bacterial biofilms are spatially structured communities that contain bacterial cells with a wide range of physiological states. The spatial distribution and speciation of copper in unsaturated Pseudomonas putida CZ1 biofilms that accumulated 147.0 mg copper per g dry weight were determined by transmission electron microscopy coupled with energy dispersive X-ray analysis, and micro-X-ray fluorescence microscopy coupled with micro-X-ray absorption near edge structure (micro-XANES) analysis. It was found that copper was mainly precipitated in a 75 μm thick layer as copper phosphate in the middle of the biofilm, while there were two living cell layers in the air-biofilm and biofilm-medium interfaces, respectively, distinguished from the copper precipitation layer by two interfaces. The X-ray absorption fine structure analysis of biofilm revealed that species resembling Cu<sub>3</sub>(PO<sub>4</sub>)<sub>2</sub> predominated in biofilm, followed by Cu-Citrate- and Cu-Glutathione-like species. Further analysis by micro-XANES revealed that 94.4% of copper were Cu<sub>3</sub>(PO<sub>4</sub>)<sub>2</sub>-like species in the layer next to the air interface, whereas the copper species of the layer next to the medium interface were composed by 75.4% Cu<sub>3</sub>(PO<sub>4</sub>)<sub>2</sub>, 10.9% Cu-Citrate-like species, and 11.2% Cu-Glutathione-like species. Thereby, it was suggested that copper was initially acquired by cells in the biofilm-air interface as a citrate complex, and then transported out and bound by out membranes of cells, released from the copper-bound membranes, and finally precipitated with phosphate in the extracellular matrix of the biofilm. These results revealed a clear spatial pattern of copper precipitation in unsaturated biofilm, which was responsible for the high copper tolerance and accumulation of the biofilm.

      • KCI등재

        Regulation of Innate Immune Response to Fungal Infection in Caenorhabditis elegans by SHN-1/SHANK

        Lingmei Sun,Huirong Li,Li Zhao,Liao Kai 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.11

        In Caenorhabditis elegans, SHN-1 is the homologue of SHANK, a scaffolding protein. In this study, we determined the molecular basis for SHN-1/SHANK in the regulation of innate immune response to fungal infection. Mutation of shn-1 increased the susceptibility to Candida albicans infection and suppressed the innate immune response. After C. albicans infection for 6, 12, or 24 h, both transcriptional expression of shn-1 and SHN-1::GFP expression were increased, implying that the activated SHN-1 may mediate a protection mechanism for C. elegans against the adverse effects from fungal infection. SHN-1 acted in both the neurons and the intestine to regulate the innate immune response to fungal infection. In the neurons, GLR-1, an AMPA ionotropic glutamate receptor, was identified as the downstream target in the regulation of innate immune response to fungal infection. GLR-1 further positively affected the function of SER-7-mediated serotonin signaling and antagonized the function of DAT-1-mediated dopamine signaling in the regulation of innate immune response to fungal infection. Our study suggests the novel function of SHN-1/SHANK in the regulation of innate immune response to fungal infection. Moreover, our results also denote the crucial role of neurotransmitter signals in mediating the function of SHN-1/SHANK in regulating innate immune response to fungal infection.

      • SCIESCOPUSKCI등재

        Effect of JAK-STAT pathway in regulation of fatty liver hemorrhagic syndrome in chickens

        Zhu, Yaling,Mao, Huirong,Peng, Gang,Zeng, Qingjie,Wei, Qing,Ruan, Jiming,Huang, Jianzhen Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.1

        Objective: To explore the molecular mechanisms of fatty liver hemorrhagic syndrome (FLHS) in laying hens, an experiment was conducted to reveal the differences in histopathological observation and gene expression between FLHS group and normal group. Methods: We compared the histopathological difference using hematoxylin and eosin staining and proceeded with RNA sequencing of adipose tissue to search differentially expressed genes and enriched biological processes and pathways. Then we validated the mRNA expression levels by real-time polymerase chain reaction and quantified protein levels in the circulation by enzyme-linked immunosorbent assay. Results: We identified 100 differentially expressed transcripts corresponding to 66 genes (DEGs) were identified between FLHS-affected group and normal group. Seven DEGs were significantly enriched in the immune response process and lipid metabolic process, including phospholipase A2 group V, WAP kunitz and netrin domain containing 2, delta 4-desaturase sphingolipid 2, perilipin 3, interleukin-6 (IL-6), ciliary neurotrophic factor (CNTF), and suppressor of cytokine signaling 3 (SOCS3). And these genes could be the targets of immune response and be involved in metabolic homeostasis during the process of FLHS in laying hens. Based on functional categories of the DEGs, we further proposed a model to explain the etiology and pathogenesis of FLHS. IL-6 and SOCS3 mediate inflammatory responses and the satiety hormone of leptin, induce dysfunction of Jak-STAT signaling pathway, leading to insulin resistance and lipid metabolic disorders. Conversely, CNTF may reduce tissue destruction during inflammatory attacks and confer protection from inflammation-induced insulin resistance in FLHS chickens. Conclusion: These findings highlight the therapeutic implications of targeting the JAK-STAT pathway. Inhibition of IL6 and SOCS3 and facilitation of CNTF could serve as a favorable strategy to enhance insulin action and improve glucose homoeostasis, which are of importance for treating obesity-related disorders for chickens.

      • KCI등재

        Full-length transcriptome combined with RNA sequence analysis of Fraxinus chinensis

        Sun Xiaochun,Li Huirong 한국유전학회 2023 Genes & Genomics Vol.45 No.5

        Background The dry root or stem bark of Fraxinus chinensis is a famous herb Qin Pi which is known for its anti-inflammatory, analgesic, anti-tumor, liver protective and diuretic pharmacological effects, the fundamental chemical components are coumarin, phenylethanol glycosides and flavonoids. However, it is difficult to clarify the secondary metabolite synthesis pathway and key genes involved in the pathway because of lack genome information of Fraxinus chinensis. Objective To generate a complete transcriptome of Fraxinus chinensis and to clarify the differentially expressed genes (DEGs) in leaves and stem barks. Methods In this study, full-length transcriptome analysis and RNA-Seq were combined to characterize Fraxinus chinensis transcriptome. Results A total of 69,145 transcripts were acquired and regarded as reference transcriptome, 67,441 transcripts (97.47%) were annotated to NCBI non-redundant protein (Nr), SwissProt, the Kyoto Encyclopedia of Genes and Genomes (KEGG) and eukaryotic orthologous groups (KOG) databases. A total of 18,917 isoforms were annotated to KEGG database and classified to 138 biological pathways. In total, 10,822 simple sequence repeat (SSRs) and 11,319 resistance (R) gene were classified to 18 types, and 3947 transcription factors (TFs) were identified in full-length transcriptome analysis. Additionally, 15,095 DEGs were detected by RNA-seq in leaves and barks, including 4696 significantly up-regulated and 10,399 significantly down-regulated genes. And 254 transcripts were annotated into phenylpropane metabolism pathway containing 86 DEGs and ten of these enzyme genes were verified by qRT-PCR. Conclusion It laid the foundation for further exploration of the biosynthetic pathway of phenylpropanoids and related key enzyme genes.

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