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Regulated Deficit Irrigation and Its Several Problems in Practical Use
( Huanjie Cai ),( Shaozhong Kang ) 한국농공학회 2000 韓國農工學會誌 : 전원과 자원 Vol.42 No.-
Regulated Deficit Irrigation (RDI) is one of the most important measures for the water-saving and high yield of crops. RDI is based on the crop and water relations. The theories of RDI were analyzed using the experiment data in Shaanxi and Gansu Province. There are several problems of RDI in practical use, which include; the uncertainty of crop-water relations, the proper growth stages and water deficit degree of RDI applied, and the requirements of RDI to irrigation system and irrigation techniques.
Regulated Deficit Irrigation and Its Several Problems in Practical Use
( Huanjie Cai ),( Shaozhong Kang ) 한국농공학회 1999 한국농공학회 학술대회초록집 Vol.1999 No.-
Regulated Deficit Irrigation (RDI) is one of the most important measures for the water-saving and high yield of crops. RDI is based on the crop and water relations. The theories of RDI were analyzed using the experiment data in Shaanxi and Gansu Province. There are several problems of RDI in practical use, which include: the uncertainty of crop-water relations, the proper growth stages and water deficit degree of RDI applied, and the requirements of RDI to irrigation system and irrigation techniques.
Silencing IL-23 expression by a small hairpin RNA protects against asthma in mice
Li, Yanchun,Sun, Meng,Cheng, Huanji,Li, Shanyu,Liu, Li,Qiao, Hongmei,Hua, Shucheng,Lu, Jirong Korean Society for Biochemistry and Molecular Bion 2011 Experimental and molecular medicine Vol.43 No.4
To determine the impact of IL-23 knockdown by RNA interference on the development and severity of ovalbumin (OVA)-induced asthmatic inflammation, and the potential mechanisms in mice, the IL-23-specific RNAi-expressing pSRZsi-IL-23p19 plasmid was constructed and inhaled into OVA-sensitized mice before each challenge, as compared with that of control mice treated with alum or budesonide. Inhalation of the pSRZsi-IL-23p19, significantly reduced the levels of OVA-challenge induced IL-23 in the lung tissues by nearly 75%, determined by RT-PCR. In addition, knockdown of IL-23 expression dramatically reduced the numbers of eosinophils and neutrophils in BALF and mitigated inflammation in the lungs of asthmatic mice. Furthermore, knockdown of IL-23 expression significantly decreased the levels of serum IgE, IL-23, IL-17, and IL-4, but not IFN${\gamma}$, and its anti-inflammatory effects were similar to or better than that of treatment with budesonide in asthmatic mice. Our data support the notion that IL-23 and associated Th17 responses contribute to the pathogenic process of bronchial asthma. Knockdown of IL-23 by RNAi effectively inhibits asthmatic inflammation, which is associated with mitigating the production of IL-17 and IL-4 in asthmatic mice.
Cooperation between Human DAF and CD59 in Protecting Cells from Human Complement-mediated Lysis
Xu, Li,Wu, Wenlan,Zhao, Zhouzhou,Shao, Huanjie,Liu, Wanhong,Liu, Hui,Li, Wenxin Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.6
The complement (C) regulatory proteins decay accelerating factor (DAF, CD55) and CD59 could protect host cells using different mechanisms from C-mediated damage at two distinct levels within the C pathway. Co-expression of DAF and CD59 would be an effective strategy to help overcome host C-induced xenograft hyperacute rejection. In this study, we made a construct of recombinant expression vector containing DAF and CD59 cDNA and the stable cell lines were obtained by G418 selection. Extraneous genes integration and co-expression were identified by PCR, RT-PCR and Western blot analysis. Human c-mediated cytolysis assays showed that NIH/3T3 cells transfected stably with pcDNA3-CD59, pcDNA3-DAF, and pcDNA3-CD59DAF-DP were protected from C-mediated damage and that synchronously expressed human CD59 and DAF provided the most excellent protection for host cells as compared with either human CD59 or DAF expressed alone. Therefore, the construct represents an effective and efficacy strategy to overcome C-mediated damage in cells and, ultimately, in animals.
Silencing IL-23 expression by a small hairpin RNA protects against asthma in mice
Yanchun Li,Shucheng Hua,Jirong Lu,Meng Sun,Huanji Cheng,Shanyu Li,Li Liu,Hongmei Qiao 생화학분자생물학회 2011 Experimental and molecular medicine Vol.43 No.4
To determine the impact of IL-23 knockdown by RNA interference on the development and severity of ovalbumin (OVA)-induced asthmatic inflammation, and the potential mechanisms in mice, the IL-23-specific RNAi-expressing pSRZsi-IL-23p19 plasmid was constructed and inhaled into OVA-sensitized mice before each challenge, as compared with that of control mice treated with alum or budesonide. Inhalation of the pSRZsi-IL-23p19, significantly reduced the levels of OVA-challenge induced IL-23 in the lung tissues by nearly 75%, determined by RT-PCR. In addition, knockdown of IL-23 expression dramatically reduced the numbers of eosinophils and neutrophils in BALF and mitigated inflammation in the lungs of asthmatic mice. Furthermore, knockdown of IL-23 expression significantly decreased the levels of serum IgE, IL-23,IL-17, and IL-4, but not IFNγ, and its anti-inflammatory effects were similar to or better than that of treatment with budesonide in asthmatic mice. Our data support the notion that IL-23 and associated Th17 responses contribute to the pathogenic process of bronchial asthma. Knockdown of IL-23 by RNAi effectively inhibits asthmatic inflammation, which is associated with mitigating the production of IL-17 and IL-4 in asthmatic mice.