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- 저자 : Herrera Miguel N. (검색결과 3 건)
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Herrera Miguel N. 한국자원공학회 2022 Geosystem engineering Vol.25 No.3
Chile is a world leader in copper production and is expected to reach production of around 6,237,000 tons of fine copper by 2022. On the other hand, in 2021 the production of copper by the hydrometallurgical route reached 1,509,000 tons and that of the smelting and refining route was 4,606,000 tons. Considering this production scenario, this article describes in a general way the contributions that the Chilean mining industry has made to the fulfillment of the 17 sustainable development goals, SDGs. The main advances are highlighted, besides discussing the main pending tasks to comply with the commitments made by Chile towards the international community.
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Marfil-Santana Miguel David,O’Connor-Sánchez Aileen,Ramírez-Prado Jorge Humberto,De los Santos-Briones Cesar,López-Aguiar,Lluvia Korynthia,Rojas-Herrera Rafael,Lago-Lestón Asunción,Prieto-Davó Alejand 한국미생물학회 2016 The journal of microbiology Vol.54 No.11
The need for new antibiotics has sparked a search for the microbes that might potentially produce them. Current sequencing technologies allow us to explore the biotechnological potential of microbial communities in diverse environments without the need for cultivation, benefitting natural product discovery in diverse ways. A relatively recent method to search for the possible production of novel compounds includes studying the diverse genes belonging to polyketide synthase pathways (PKS), as these complex enzymes are an important source of novel therapeutics. In order to explore the biotechnological potential of the microbial community from the largest underground aquifer in the world located in the Yucatan, we used a polyphasic approach in which a simple, non-computationally intensive method was coupled with direct amplification of environmental DNA to assess the diversity and novelty of PKS type I ketosynthase (KS) domains. Our results suggest that the bioinformatic method proposed can indeed be used to assess the novelty of KS enzymes; nevertheless, this in silico study did not identify some of the KS diversity due to primer bias and stringency criteria outlined by the metagenomics pipeline. Therefore, additionally implementing a method involving the direct cloning of KS domains enhanced our results. Compared to other freshwater environments, the aquifer was characterized by considerably less diversity in relation to known ketosynthase domains; however, the metagenome included a family of KS type I domains phylogenetically related, but not identical, to those found in the curamycin pathway, as well as an outstanding number of thiolases. Over all, this first look into the microbial community found in this large Yucatan aquifer and other fresh water free living microbial communities highlights the potential of these previously overlooked environments as a source of novel natural products.
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Santy Peraza-Echeverria,Jorge M. Santamaría,Gabriela Fuentes,Mariana de los Ángeles Menéndez-Cerón,Miguel Ángel Vallejo-Reyna,Virginia Aurora Herrera-Valencia 한국유전학회 2012 Genes & Genomics Vol.34 No.4
The NPR1 (non-expressor of pathogenesis related gene 1) gene was initially identified in Arabidopsis as a master regulator of the systemic acquired resistance (SAR). Five additional NPR1 homologues have been identified in Arabidopsis whose function range from regulators of SAR to plant development. In the present study, we characterized the structure, phylogeny and expression of the NPR1 family in papaya (Carica papaya L.), one of the most important tropical fruit crops. We identified four NPR1 homologues in the papaya genome sequence (CpNPR1 to CpNPR4). Overall, the four papaya predicted NPR1 proteins showed the characteristic BTB/POZ and ankyrin domains of the Arabidopsis NPR1 family. Twelve additional open reading frames showing homology to retrotransposon elements or genes involved in different physiological processes were found in close proximity to the papaya NPR1homologues. The phylogenetic analysis revealed that the papaya NPR1 sequences resolved in three clades, each clade containing two Arabidopsis NPR1 homologues involved either in the positive regulation of SAR (clade I), negative regulation of SAR (clade II) or plant development (clade III), suggesting a similar function for the corresponding papaya NPR1homologues. Furthermore, the expression of the four papaya NPR1 homologues was detected in both vegetative and reproductive tissues. The present study has provided the first comparative analysis of the NPR1 family in a tropical fruit crop and expanded our knowledge on this type of genes in dicotyledoneous plants. The identification of the full set of papaya NPR1 homologues will pave the way for their systematic functional analysis and new opportunities for engineering disease resistance in this crop.
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