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Michael J. Simmonds,Rhys Christy,Sonya M. Marshall-Gradisnik,Herbert J. Meiselman,Oguz K. Baskurt 한국유변학회 2011 Korea-Australia rheology journal Vol.23 No.4
The extent of red blood cell (RBC) aggregation has widely been accepted as a reliable indicator of inflammatory processes. The ability to measure RBC aggregation indexes in capillary blood samples may simplify the application of the test and may bring advantages in its clinical usage as well as application in field studies. This study compared RBC aggregation indexes measured in venous and capillary blood samples using a newly developed capillary tube aggregometer. Although strong correlations between aggregation indexes measured in capillary and venous samples were detected, the indexes measured in venous samples were higher compared to capillary samples, thus indicating a bias. The possible reason for this bias is proposed to be related to the difference in oxygen saturation of venous and capillary samples and therefore altered the light absorption spectrums. Our results indicate that capillary blood samples can be used to estimate RBC aggregation in venous blood samples, however the bias should be taken into account.
Simmonds, Michael J.,Christy, Rhys,Marshall-Gradisnik, Sonya M.,Meiselman, Herbert J.,Baskurt, Oguz K. 한국유변학회 2011 Korea-Australia rheology journal Vol.23 No.4
The extent of red blood cell (RBC) aggregation has widely been accepted as a reliable indicator of inflammatory processes. The ability to measure RBC aggregation indexes in capillary blood samples may simplify the application of the test and may bring advantages in its clinical usage as well as application in field studies. This study compared RBC aggregation indexes measured in venous and capillary blood samples using a newly developed capillary tube aggregometer. Although strong correlations between aggregation indexes measured in capillary and venous samples were detected, the indexes measured in venous samples were higher compared to capillary samples, thus indicating a bias. The possible reason for this bias is proposed to be related to the difference in oxygen saturation of venous and capillary samples and therefore altered the light absorption spectrums. Our results indicate that capillary blood samples can be used to estimate RBC aggregation in venous blood samples, however the bias should be taken into account.
Nemeth, Norbert,Baskurt, Oguz K.,Meiselman, Herbert J.,Furka, Istvan,Miko, Iren The Korean Society of Rheology 2009 Korea-Australia rheology journal Vol.21 No.3
Micropore filtration of dilute red blood cell (RBC) suspensions is a widely known method for determining red blood cell deformability. Use of this method for cells from various laboratory animal species does require considering the effects of the cell size to pore size ratio and of suspension hematocrit. In general, previous animal studies have utilized 5% hematocrit suspensions and five micron pores, and thus conditions similar to human clinical laboratory practice. However, when used for repeated sampling from small laboratory animals or for parallel multiple samples from different sites in large laboratory animals, the volume of blood sampled and hence the hematocrit of the test suspension may be limited. Our results indicate that hematocrit levels yielding stable values of RBC pore transit time are pore size and species specific: three micron pores = $2{\sim}5%$ for dog and $3{\sim}5%$ for rat; five micron pores $3{\sim}5%$ for dog and $1{\sim}5%$ for rat. An analytical approach using a common expression for calculating transit time is useful for determining the sensitivity of this time to hematocrit alterations and hence to indicate hematocrit levels that may be problematic.
Norbert Nemeth,Oguz K. Baskurt,Herbert J. Meiselman,Istvan Furka,Iren Miko 한국유변학회 2009 Korea-Australia rheology journal Vol.21 No.3
Micropore filtration of dilute red blood cell (RBC) suspensions is a widely known method for determining red blood cell deformability. Use of this method for cells from various laboratory animal species does require considering the effects of the cell size to pore size ratio and of suspension hematocrit. In general, previous animal studies have utilized 5% hematocrit suspensions and five micron pores, and thus conditions similar to human clinical laboratory practice. However, when used for repeated sampling from small laboratory animals or for parallel multiple samples from different sites in large laboratory animals, the volume of blood sampled and hence the hematocrit of the test suspension may be limited. Our results indicate that hematocrit levels yielding stable values of RBC pore transit time are pore size and species specific: three micron pores=2~5% for dog and 3~5% for rat; five micron pores 3~5% for dog and 1~5% for rat. An analytical approach using a common expression for calculating transit time is useful for determining the sensitivity of this time to hematocrit alterations and hence to indicate hematocrit levels that may be problematic.
Nemeth, Norbert,Baskurt, Oguz K.,Meiselman, Herbert J.,Kiss, Ferenc,Uyuklu, Mehmet,Hever, Timea,Sajtos, Erika,Kenyeres, Peter,Toth, Kalman,Furka, Istvan,Miko, Iren The Korean Society of Rheology 2009 Korea-Australia rheology journal Vol.21 No.2
Hemorheological results may be influenced by the time between blood sampling and measurement, and storage conditions (e.g., temperature, time) during sample delivery between laboratories may further affect the resulting data. This study examined possible hemorheological alterations subsequent to storage of rat and dog blood at room temperature ($22^{\circ}C$) or with cooling ($4{\sim}10^{\circ}C$) for 2, 4, 6, 24, 48 and 72 hours. Measured hemorheological parameters included hematological indices, RBC aggregation and RBC deformability. Our results indicate that marked changes of RBC deformability and of RBC aggregation in whole blood can occur during storage, especially for samples stored at room temperature. The patterns of deformability and aggregation changes at room temperature are complex and species specific, whereas those for storage at the lower temperature range are much less complicated. For room temperature storage, it thus seems logical to suggest measuring rat and dog cell deformability within 6 hours; aggregation should be measured immediately for rat blood or within 6 hours for dog blood. Storage at lower temperatures allows measuring EI up to 72 hours after sampling, while aggregation must be measured immediately, or if willing to accept a constant decrease, over 24~72 hours.
Norbert Nemeth,Oguz K. Baskurt,Herbert J. Meiselman,Ferenc Kiss,Mehmet Uyuklu,Timea Hever,Erika Sajtos,Peter Kenyeres,Kalman Toth,Istvan Furka,Iren Miko 한국유변학회 2009 Korea-Australia rheology journal Vol.21 No.2
Hemorheological results may be influenced by the time between blood sampling and measurement, and storage conditions (e.g., temperature, time) during sample delivery between laboratories may further affect the resulting data. This study examined possible hemorheological alterations subsequent to storage of rat and dog blood at room temperature (22℃) or with cooling (4~10℃) for 2, 4, 6, 24, 48 and 72 hours. Measured hemorheological parameters included hematological indices, RBC aggregation and RBC deformability. Our results indicate that marked changes of RBC deformability and of RBC aggregation in whole blood can occur during storage, especially for samples stored at room temperature. The patterns of deformability and aggregation changes at room temperature are complex and species specific, whereas those for storage at the lower temperature range are much less complicated. For room temperature storage, it thus seems logical to suggest measuring rat and dog cell deformability within 6 hours; aggregation should be measured immediately for rat blood or within 6 hours for dog blood. Storage at lower temperatures allows measuring EI up to 72 hours after sampling, while aggregation must be measured immediately, or if willing to accept a constant decrease, over 24~72 hours.