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Heeyoun Ahn 한국외국어대학교 통번역연구소 2022 한국외국어대학교 통번역연구소 학술대회 Vol.2022 No.01
The purpose of this research is to suggest types of interpreters’ roles and the boundary of the roles, which is required for business interpretation. In business interpretation, both interpreters and their users are active regarding their roles as cultural mediators. However, there hasn’t been an answer for the roles of interpreters who are not biased but reliable. This suggests that interpreters and their users are exposed to the conflicts on their roles. To resolve this problem, this research conducts theoretical reviews and surveys in parallel. By studying types of interpretation and theories related to interpreters’ roles in various perspectives, the study is able to draw core standards of types of interpretation, which helps establish the analysis framework of this study. Surveys for this study were conducted to see the interpreters’ perception on their roles as cultural mediators in business interpretation and the expectation of users. First, a survey reviewed interpreters’ own perception on their roles. By combining the survey results with implications from prior research, the study selected a few interpreters who can discuss different perceptions of different interpreters’ roles as they have experience as both freelancers and in-house interpreters. Another survey was conducted to collect answers from users of interpreters: these users have experience of using interpretation service for cultural and linguistic communications where global business happens. Thus, such users were able to share their thoughts regarding the roles of business interpreters. With the mentioned methods, this research confirms that interpreters are implementing their roles going over the boundary of various roles of theirs in the grey area of business interpretation where clear boundarys of roles can’t be expected despite a little clue. The research also confirms that the users of interpreters assign interpreters with the expected roles as cultural mediators based on their ideas that the users are only able to have smooth communications through the cultural coordination of interpreters during the intercultural business communications. This means that it is necessary for business interpreters to be aware of their roles as cultural mediators and for users to understand and have empathy for interpreters’ roles. To that end, professional organizations for interpreting education need to deliver the relevant knowledge by reflecting what happens on site of business interpretation so that interpreters-to-be can realize the ideal roles that users expect and implement what they learn on site. A guideline for the roles of interpreters will be handy to elicit collaboration with interpreters by making users understood with the interpretation process and roles of interpreters. (Ewha Womans University, Korea)
Heeyoun Hwang,Ju Yeon Lee,Hyun Kyoung Lee,Gun Wook Park,Hoi Keun Jeong,Myeong Hee Moon,Jin Young Kim,Jong Shin Yoo 한국당과학회 2017 한국당과학회 학술대회 Vol.2017 No.01
The characterization of site-specific micro-heterogeneity in glycoprotein is very important for understanding cell biology and disease processes. Vitronectin is well known to be a multi-functional glycoprotein in blood and the extracellular matrix, which is related to hepatocellular carcinoma (HCC). Here, we systematically analyzed the site-specific N-glycopeptides of vitronectin in human plasma by tandem mass spectrometry combined with immunoprecipitation and HILIC enrichment. Vitronectin was purified with immunoprecipitation by monoclonal antibody from plasma and digested to tryptic N-glycopeptides. Then, enrichment with HILIC materials was used, and followed by analysis with nano-LC/MS/MS. The sequences of N-glycopeptides were identified from the mass spectra by high-energy C-trap dissociation (HCD) and collision-induced dissociation (CID). In HCD mode, oxonium ions were used for recognizing glycopeptides and y ions for sequencing the peptide backbone. In CID mode, Y ions were used for characterizing their glycoforms. As a result, total 17 site-specific N-glycopeptides were completely identified at all of three N-glycosylation sites of vitronectin in human plasma, including 12 N-glycopeptides first reported. Finally, we specifically found that three hybrid and four complex glycopeptides of tri-antennary forms with outer-fucosylation increased in HCC human plasma.
Chromosome-Centric Human Proteome Study of Chromosome 11 Team
( Heeyoun Hwang ),( Jin Young Kim ),( Jong Shin Yoo ) 한국질량분석학회 2021 Mass spectrometry letters Vol.12 No.3
As a part of the Chromosome-centric Human Proteome Project (C-HPP), we have developed a few algorithms for accurate identification of missing proteins, alternative splicing variants, single amino acid variants, and characterization of function unannotated proteins. We have found missing proteins, novel and known ASVs, and SAAVs using LC-MS/MS data from human brain and olfactory epithelial tissue, where we validated their existence using synthetic peptides. According to the neXtProt database, the number of missing proteins in chromosome 11 shows a decreasing pattern. The development of genomic and transcriptomic sequencing techniques make the number of protein variants in chromosome 11 tremendously increase. We developed a web solution named as SAAvpedia for identification and function annotation of SAAVs, and the SAAV information is automatically transformed into the neXtProt web page using REST API service. For the 73 uPE1 in chromosome 11, we have studied the function annotaion of CCDC90B (NX_Q9GZT6), SMAP (NX_O00193), and C11orf52 (NX_Q96A22).
Biochemical and NMR Characterization of MTH1880 Mutant Proteins for Folding-Unfolding Studies
Heeyoun Kim,Sooyoung Ryu,윤지혜,김석만,장익수,이원태 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.12
MTH1880 is a hypothetical protein derived from Methanobacterium thermoautotrophicum, thermophilic methanogen. The solution structure determined by NMR spectroscopy showed that it has a novel α+β-fold with a highly acidic ligand binding pocket. Since MTH1880 maintains its ultra-stable structural characteristics at both high temperature and pressure, it has been considered as an excellent model for studying protein folding. To initiate the structural and folding study of MTH1880 in proving its unusual stability, we performed the site directed mutagenesis and biochemical analysis of MTH1880 mutants. Data from circular dichroism and NMR spectroscopy suggest that the point mutations perturbed the structural stability of protein even though the secondary structure is retained. This study will provide the useful information in understanding the role of participating residues during folding-unfolding process and our result will be used in designing further folding experiments for hyper-thermopile proteins like MTH1880.
Heeyoun Hwangl,Unyong Kim,Young Suk Seol,Myung Jin Oh,Hyun Joo An 한국당과학회 2018 한국당과학회 학술대회 Vol.2018 No.07
Heterogeneity and complexity of the glycosylation on biotherapeutics greatly depend on expression system, process conditions, and environment of cell culture of products. In order to evaluate biosimilarity of antibody drug such as Herceptin®, Remicade®, and Remsima®, we have developed a deep learning model, Deep Y, using intact glycoprotein analysis by LC-MS. Briefly, each antibody drug was independently analyzed to identify its intact glycoprotein composition. As a result, the list of identified intact glycoprotein compositions from each MS data was merged in the intact glycoprotein database, where a total of 34 intact glycoprotein compositions was identified from all three antibody drugs. Independently, the deconvoluted masses and their abundances of antibody drugs generated by Mass Hunter were used as data sets such as training, validation, and test set, for development of a deep learning model using convolutional and fully connected neural network. The accuracy was 100%, 90% and 85% at training, validation and test set, respectively, where the experiments of more than 100 ng/ml were predicted as 100% of accuracy at test set. The DeepY could predict the biosimilarity and distinguish the low quality of mass spectra from all antibody drugs. We will further test the antibody drugs in batch to batch, expand to other original antibody drugs and their biosimilars.