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- 저자 : Haq Ikram-ul (검색결과 4 건)
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Bioloistic-mediated Transformation of Cotton (Gossypium hirsutum L.): Embryogenic Calli as Explant
Haq Ikram-ul,Asad Shaheen,Zafar Yusuf The Korean Society of Plant Biotechnology 2005 Plant molecular biology and biotechnology research Vol.7 No.4
Genetic transformation was carried out by using biolistic gun method. The hypocotyl derived embryogenic calli (explants) of cotton (Gossypium hirsutum L.) cv. Cocker-312 were transformed with a recombinant pGreen II plasmid, in which both, bar (selection marker) and GUS (${\beta}$-glucuronidase) reporter genes were incorporated. Explants were arranged on osmoticum-containing medium (0.5M mannitol) 4 hours prior to and 16 hours after bombardment that was resulted into an increase about >80% for GUS stable expression. 3 days after bombardment, GUS assay was performed, which exhibited, $18.36{\pm}1.00$ calli showed blue spots. The transformed embryogenic calli were cultured on selection medium (@ 6 mg/L basta) for 3 months. The putative transgenic plants were developed via selective somatic embryogenesis (@1.50 mg/L basta); maximum $27.58{\pm}1.25$ somatic embryos were obtained while $17.47{\pm}1.00$ embryos developed into plantlets (@ 0.75mg/L basta). In five independent experiments, up to 7.24% transformation efficiency was recorded. The presence of the transgenes was analyzed by using PCR and southern hybridization analysis. The transgenic plants were developed with in 6-7 months, but mostly transformants were abnormal in morphology.
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Haq, Ikram-ul,Zafar, Yusuf The Korean Society of Plant Biotechnology 2004 Plant molecular biology and biotechnology research Vol.6 No.1
Callus induction and somatic embryogenesis are fundamental to cotton tissue culture biotechnology. An efficient protocol for callus induction, somatic embryogenesis and their maturation have been developed to regenerate plantlets from cotton (Gossypium hirsutum L.) variety coker 312. Embryogenic callus was initiated from hypo-cotyl region that was used as an explant at seedling stage when it was about 7-8 days old. Callus induction was achieved through culturing hypocotyls (5-7mm) on $MS_{1a} medium supplemented with 2,4-D (0.1 mg/L) and KT (0.5 mg/L) for six weeks. A friable, colorless, bulky and well proliferating callus becomes greenish with the addition of NAA (2.0 mg/L), ZT (0.1 mg/L) and removal of 2,4-D (M $S_{1b}$) cultured for two weeks then again transferred to $MS_{1a}. 2,4-dichlorophenoxyacetic acid (2,4-D) promoted the proliferation of embryogenic callus, but had a negative effect on the differentiation and germination of somatic embryos. ZT (0.1mg/L) and activated charcoal (2g/L), both hormones play an important role in differentiation and germination of somatic embryos in hypocotyls derived embryogenic callus but in case of cotton, such a capability have been observed on MS medium with 1.92 g/L $KNO_3$, but it is considered to attain somewhat more improvement. High embryogenesis frequency was achieved through nutrient deficient stress treatment. The frequency of globular embryogenesis (two-three folds) was achieved when well proliferating callus was (from $MS_{1a}$ media) cultured on MS (1/5 strength) medium for four weeks. Here the development of anthocyanins is the best indicator for somatic embryogenesis. However, when embryoid callus was cultured on MS (full strength) medium, the globular embryos were developed into normal plantlets immediately. In this procedure 27.49% cotyledenary embryos were developed. Of that 70% cotyledenary embryos were developed not only into normal plantlets but rooted simultaneously, when cultured on MS (with 0.05 mgg/L giberrelic acid) medium. So complete plants could be regenerated through somatic embryogenesis from hypocotyl explants within 6 months.s.
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Siraj-ul-Islam,Fazal-i-Haq,Ikram A. Tirmizi 한국전산응용수학회 2010 Journal of applied mathematics & informatics Vol.28 No.3
A Numerical scheme based on collocationmethod using quartic B-spline functions is designed for the numerical solution of one-dimensional modified equal width wave (MEW) wave equation. Using Von-Neumann approach the scheme is shown to be unconditionally stable. Performance of the method is validated through test problems including single wave,interaction of two waves and use of Maxwellian initial condition. Using error norms L2 and L1 and conservative properties of mass, momentum and energy, accuracy and efficiency of the suggested method is established through comparison with the existing numerical techniques.
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Islam, Siraj-Ul,Haq, Fazal-I,Tirmizi, Ikram A. The Korean Society for Computational and Applied M 2010 Journal of applied mathematics & informatics Vol.28 No.3
A Numerical scheme based on collocation method using quartic B-spline functions is designed for the numerical solution of one-dimensional modified equal width wave (MEW) wave equation. Using Von-Neumann approach the scheme is shown to be unconditionally stable. Performance of the method is validated through test problems including single wave, interaction of two waves and use of Maxwellian initial condition. Using error norms $L_2$ and $L_{\infty}$ and conservative properties of mass, momentum and energy, accuracy and efficiency of the suggested method is established through comparison with the existing numerical techniques.
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