Identification of Novel Endo-β-1,4-glucanase Isoforms fromBursaphelenchus Species (Nemtoda: Aphelenchoididae)

Dae-Weon Lee,Il Sung Moon,강재순,Hyun Jung Choe,최재영,제연호,이시혁,Hye Rim Han,Kook-Jin Lim6,Young Ho Koh 한국응용곤충학회 2007 Journal of Asia-Pacific Entomology Vol.10 No.2

Bursaphelenchus xylophilus has been recognized as a causal pathogen of pine wilt disease (PWD). In order to identify relevant molecular biomarkers, we selected a variable region of endo-β-1,4-glucanase (β14-gcn) which was cloned from the cDNA of B. xylophilus and B. mucronatus. We then identified three novel β14-gcn isoforms: Bm-β14-gcn and Bm-β14-gcni from B. mucronatus, and Bx-β14-gcn from B. xylophilus. Bm-β14-gcn and Bx-β14-gcn were found to be identical, whereas Bm-β14-gcni was unique to B. mucronatus. These isoforms are Bursaphelenchus-specific and may be employed as molecular markers for the diagnosis of PWD. Our phylogenetic analysis showed that these β14-gcns from Bursaphelenchus species were associated most closely with fungal β14-gcns.

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Sung Youn Jo,Mohamed Bayome,Justyn Park,Hee Jin Lim,Yoon-Ah Kook,Seong Ho Han 대한치과교정학회 2019 대한치과교정학회지 Vol.49 No.1

Effect of a Non-polymer Titanium Dioxide Thin Film-Coated Stent with Heparin in a Porcine Coronary Restenosis Model

Lim, Kyung Seob,Jeong, Myung Ho,Bae, In Ho,Park, Jun-Kyu,Park, Dae Sung,Shim, Jae Won,Kim, Han Byual,Jin, Mi Rim,Kim, Hyun Kuk,Kim, Sung Soo,Kim, Min Chul,Sim, Doo Sun,Hong, Young Joon,Kim, Ju Han,Ahn The Korean Society on Thrombosis and Hemostasis 2017 Clinical & experimental thrombosis and hemostasis Vol.3 No.2

Clinical relevance of combined anti-mitochondrial M2 detection assays for primary biliary cirrhosis

Han, Eunhee,Jo, Sung Jin,Lee, Hyeyoung,Choi, Ae-Ran,Lim, Jihyang,Jung, Eun-Sun,Oh, Eun-Jee Elsevier 2017 Clinica chimica acta Vol.464 No.-

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Antimitochondrial antibody (AMA) is a specific serologic marker in primary biliary cirrhosis (PBC). The aim of this study was to evaluate the clinical relevance of combined AMA assays.</P> <P><B>Methods</B></P> <P>Sera were obtained from 79 patients with PBC and 108 patients with other liver disease. They were tested by indirect immunofluorescence (IIF) using rat kidney/stomach tissue and HEp2 cells as substrate, 4 AMA-M2 assays, anti-sp100, and anti-gp210 assays.</P> <P><B>Results</B></P> <P>Using IIF-AMA with cut-off titer of 1:40, the sensitivity and specificity for PBC were 88.6% and 87.0%, respectively. A cut-off titer of 1:80 improved the specificity to 93.5%. The 4 commercial assay kits using AMA-M2 autoantibodies showed sensitivity of 55.7–79.7% and specificity of 91.7–95.4% with moderate to good agreement. AMA-M2 assays using both native and recombinant E2 antigens had higher sensitivity. ANAs on HEp2 cells, anti-sp100, and anti-gp210 were detected in 67.1%, 13.9–15.2%, and 22.8–27.8% of PBC patients, respectively. Additional AMA-M2 specific assays in IIF-AMA negative and low titer positive (1:40) sera increased the sensitivity and specificity to 88.6% and 90.7%, respectively.</P> <P><B>Conclusions</B></P> <P>Serological diagnosis for PBC using IIF with high titer cut-off and additional AMA-M2 specific tests by ELISA or LIA in IIF-negative sera should be used.</P> <P><B>Highlights</B></P> <P> <UL> <LI> IIF with cut-off value of 1:80 has high specificity for AMA detection. </LI> <LI> Assays using recombinant E3 antigens had high sensitivity for PBC diagnosis. </LI> <LI> IIF with high titer cut-off and additional M2 specific tests in IIF-negative sera should be used for PBC diagnosis. </LI> </UL> </P>

Lysyl–Transfer RNA Synthetase Induces the Maturation of Dendritic Cells through MAPK and NF-κB Pathways, Strongly Contributing to Enhanced Th1 Cell Responses

Lim, Hui Xuan,Jung, Hak-Jun,Lee, Arim,Park, Si Hoon,Han, Byung Woo,Cho, Daeho,Kim, Tae Sung The American Association of Immunologists, Inc. 2018 JOURNAL OF IMMUNOLOGY Vol.201 No.9

<P>In addition to essential roles in protein synthesis, lysyl–tRNA synthetase (KRS) is secreted to trigger a proinflammatory function that induces macrophage activation and TNF-α secretion. KRS has been associated with autoimmune diseases such as polymyositis and dermatomyositis. In this study, we investigated the immunomodulatory effects of KRS on bone marrow–derived dendritic cells (DCs) of C57BL/6 mice and subsequent polarization of Th cells and analyzed the underlying mechanisms. KRS-treated DCs increased the expression of cell surface molecules and proinflammatory cytokines associated with DC maturation and activation. Especially, KRS treatment significantly increased production of IL-12, a Th1-polarizing cytokine, in DCs. KRS triggered the nuclear translocation of the NF-κB p65 subunit along with the degradation of IκB proteins and the phosphorylation of MAPKs in DCs. Additionally, JNK, p38, and ERK inhibitors markedly recovered the degradation of IκB proteins, suggesting the involvement of MAPKs as the upstream regulators of NF-κB in the KRS-induced DC maturation and activation. Importantly, KRS-treated DCs strongly increased the differentiation of Th1 cells when cocultured with CD4<SUP>+</SUP> T cells. The addition of anti–IL-12–neutralizing Ab abolished the secretion of IFN-γ in the coculture, indicating that KRS induces Th1 cell response via DC-derived IL-12. Moreover, KRS enhanced the OVA-specific Th1 cell polarization in vivo following the adoptive transfer of OVA-pulsed DCs. Taken together, these results indicated that KRS effectively induced the maturation and activation of DCs through MAPKs/NF-κB–signaling pathways and favored DC-mediated Th1 cell response.</P>

Tunable piezoelectric nanogenerators using flexoelectricity of well-ordered hollow 2D MoS₂ shells arrays for energy harvesting

Han, Jin Kyu,Kim, Sungho,Jang, Seunghun,Lim, Yi Rang,Kim, Sang-Woo,Chang, Hyunju,Song, Wooseok,Lee, Sun Sook,Lim, Jongsun,An, Ki-Seok,Myung, Sung Elsevier 2019 Nano energy Vol.61 No.-

<P><B>Abstract</B></P> <P>Piezoelectric two-dimensional (2D) transition-metal dichalcogenides such as molybdenum disulfide (MoS<SUB>2</SUB>) have recently attracted significant attention owing to their applicability for fabrication of flexible power generators. In this study, novel piezoelectric nanogenerators (PNGs) consisting of 2D piezoelectric MoS<SUB>2</SUB> shells are fabricated where an Al<SUB>2</SUB>O<SUB>3</SUB> thin layer deposited on the surface of polystyrene (PS) beads is used to avoid collapse of the spherical MoS<SUB>2</SUB> shells under the high growth temperature. In addition, the MoS<SUB>2</SUB> shell size is controlled by adjusting the PS bead size and the effects of the MoS<SUB>2</SUB> shell size on power generation characteristics are investigated. Our PNG based on the piezoelectric MoS<SUB>2</SUB> shells produces a peak output voltage of approximately 1.2 V at a pressure of 4.2 kPa. The minimum pressure for power generation by tapping is 0.3 kPa. This novel method is very promising for development of the next-generation PNGs based on 2D semiconductor piezoelectric materials.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Novel piezoelectric nanogenerators (PNGs) consisting of 2D piezoelectric MoS<SUB>2</SUB> shells were fabricated. </LI> <LI> The effect of the MoS<SUB>2</SUB> shell size on power generation characteristics was investigated. </LI> <LI> The output voltage of 1.2 V was measured under the pressure of 4.2 kPa by using MoS<SUB>2</SUB> shell-based PNGs. </LI> <LI> High-power generation properties and nonlinear hysteresis behavior were observed for the micrometer-scale MoS<SUB>2</SUB> shells. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

2015 Nationwide Survey Revealed Barley stripe mosaic virus in Korean Barley Fields

Lim, Hyo-Jin,Seo, Eun-Young,Kim, Hyun-Seung,Kim, Jung-Kyu,Park, Chan-Hwan,Gong, Jun-Su,Kim, Ik-hyun,Han, Sang-huk,Kilcrease, James P.,Furuya, Naruto FACULTY OF AGRIC PUBLICATIONS-KYUSHU UNIV 2016 Journal of the Faculty of Agriculture, Kyushu Univ Vol.61 No.1

<P>A seed transmitted virus has consistently caused significant economic damage to barley crops in Korea in recent years, and may be increasing because many farmers save seed for replanting. Because some barley seed is imported, there is the potential for introduction of new seed transmitted viruses, causing diseases which may spread. Barley cultivation in South Korea is expanding nationwide due to the increasing popularity of health foods, so both production and quality of barley grain is important. Although Barley stripe mosaic virus (BSMV) has been reported previously as a small percentage of all barley viruses present in Korea, increases in imports of barley seed may lead to increased occurrence of this seed transmitted virus. We therefore investigated the virus status of barley crops around Iksan and Wanju. On several newly cultivated barley farms, we observed areas showing symptoms typical of BSMV, and confirmed BSMV infection in 24% of the samples examined. In order to understand the occurrence and seed transmission of Korean BSMV isolates, we examined sequence variation within the Triple Gene Block proteins, and subcellular localization of two of these proteins. The newly identified Korean BSMV isolates show low sequence variability and high sequence homology to previously reported US isolates. With these results, we expect to confirm distribution of barley viruses and possible emerging viruses, which will serve as base line data to document virus prevention and control measures.</P>

Interfacial Engineering for Enhanced Light Absorption and Charge Transfer of a Solution-Processed Bulk Heterojunction Based on Heptazole as a Small Molecule Type of Donor

Lim, Iseul,Bui, Hoa Thi,Shrestha, Nabeen K.,Lee, Joong Kee,Han, Sung-Hwan American Chemical Society 2016 ACS APPLIED MATERIALS & INTERFACES Vol.8 No.13

<P>In the present study, a solution-processed organic semiconductor based on indolocarbazole derivative (heptazole) is introduced as a p-type donor material for a bulk-heterojunction photovoltaic device. The heptazole has an optical band gap of 2.97 eV, and its highest occupied molecular orbital lowest unoccupied molecular orbital energy levels are compactable with the PC60BM to construct a donor acceptor heterojuction for energy harvesting and transfer. When the bulk-heterojunction photovoltaic devices consisting of ITO/PEDOT:PSS/heptazole:PC60BM/Al with different blending ratio of heptazole:PC60BM were constructed, the cell with 1:1 blending ratio exhibited the best power conversion efficiency. Further, when an indoline organic dye (D149) was introduced as an interfacial modifier to the above donor/acceptor bulk heterojunction, the device demonstrated an enhanced overall power conversion efficiency from 1.26% to 2.51% hence demonstrating enhancement by the factor of 100%. The device was further characterized using electronic absorption spectroscopy, photoluminescence spectroscopy, electrochemical impedance spectroscopy, and the photovoltage decay kinetics. These studies reveal that the enhanced power conversion efficiency of the device is due to the enhanced charge transfer with the complementary light absorption feature of the interfacial D149 dye molecules.</P>

Amorphous Li-Zr-O layer coating on the surface of high-Ni cathode materials for lithium ion batteries

Lim, Young Jin,Lee, Sun-Me,Lim, Hayoung,Moon, Bora,Han, Kyu-Sung,Kim, Jin-Ho,Song, Jun-Ho,Yu, Ji-Sang,Cho, Woosuk,Park, Min-Sik Elsevier 2018 ELECTROCHIMICA ACTA Vol.282 No.-

<P><B>Abstract</B></P> <P>Improving cycle performance and rate-capability of lithium ion batteries is necessary in moving toward their use in electric vehicles. Working towards this goal, we propose an amorphous Li-Zr-O coating on the surface of a high-Ni layered transition metal oxide (LiNi<SUB>0.8</SUB>Co<SUB>0.1</SUB>Mn<SUB>0.1</SUB>O<SUB>2</SUB>) <I>via</I> a simple wet process. By using residual Li on the surface of as-prepared LiNi<SUB>0.8</SUB>Co<SUB>0.1</SUB>Mn<SUB>0.1</SUB>O<SUB>2</SUB> particles as a Li source, a non-stoichiometric Li-Zr-O compound can be successfully formed on the surface of LiNi<SUB>0.8</SUB>Co<SUB>0.1</SUB>Mn<SUB>0.1</SUB>O<SUB>2</SUB> particles at a low temperature of 400 °C. The formation of an amorphous Li-Zr-O layer is beneficial in minimizing undesirable side reactions associated with residual Li at the surface and, by extension, improving the cycle performance and rate-capability of LiNi<SUB>0.8</SUB>Co<SUB>0.1</SUB>Mn<SUB>0.1</SUB>O<SUB>2</SUB>. The amorphous Li-Zr-O coated LiNi<SUB>0.8</SUB>Co<SUB>0.1</SUB>Mn<SUB>0.1</SUB>O<SUB>2</SUB> exhibits a high reversible capacity of more than 190 mAh g<SUP>−1</SUP> with improved cycle performance. This simple approach offers easy control of the surface characteristics of LiNi<SUB>0.8</SUB>Co<SUB>0.1</SUB>Mn<SUB>0.1</SUB>O<SUB>2</SUB>, improving the surface stability and enhancing the thermal stability.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A non-stoichiometric Li-Zr-O compound is introduced on the surface of NCM811 particles <I>via</I> a wet-process at a temperature of 400 °C. </LI> <LI> An amorphous Li-Zr-O layer is beneficial in minimizing undesirable side reactions associated with residual Li. </LI> <LI> The amorphous Li-Zr-O coated NCM811 exhibits a high reversible capacity of 190 mAh g<SUP>−1</SUP> and stable cycle performance. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>Amorphous Li-Zr-O coating layers can be uniformly formed on the surface of the particles, which is advantageous in providing suitable Li<SUP>+</SUP> pathways and improving structural stability. In this work, the residual Li on the surface of as-prepared LiNi<SUB>0.8</SUB>Co<SUB>0.1</SUB>Mn<SUB>0.1</SUB>O<SUB>2</SUB> particles can be partially utilized as a Li source for the formation of an amorphous Li-Zr-O coating layer during the coating process.</P> <P>[DISPLAY OMISSION]</P>

Fault Current Limiting Characteristics of Transformer-Type Superconducting Fault Current Limiter Due to Winding Direction of Additional Circuit

Han, Tae-Hee,Ko, Seck-Cheol,Lim, Sung-Hun IEEE 2018 IEEE transactions on applied superconductivity Vol.28 No.3

<P>In this paper, the transformer-type superconducting fault current limiter (SFCL) with additional circuit, which could limit the fault current through twice quench operations, was suggested. The suggested SFCL includes one primary winding, two nonisolated secondary windings wound on the same iron core, and two high-<I>T<SUB>C</SUB></I> superconducting elements connected with each secondary winding. The advantage of the suggested SFCL is that it can perform the twice current limiting operations due to the transient amplitude of the fault current. Among its design parameters, the winding direction of another secondary winding comprising the additional circuit of the transformer-type SFCL or the nonisolated secondary winding is expected to affect its fault current limiting characteristics. To analyze the dependence of the fault current limiting operation on the winding direction of two nonisolated secondary windings, two limiting operational currents from its electrical equivalent circuit were derived. Through the fault current limiting tests for the suggested SFCL, the fault current limiting characteristics of the SFCL were discussed.</P>