Evaluation of Inhibitory Effect of Phlorotannins from Ecklonia cava on Triglyceride Accumulation in Adipocyte

Kim, Haejin,Kong, Chang-Suk,Lee, Jung Im,Kim, Hojun,Baek, Seungoh,Seo, Youngwan American Chemical Society 2013 Journal of agricultural and food chemistry Vol.61 No.36

<P>In the present study, a methanolic extract of Ecklonia cava and its solvent-partitioned fractions were evaluated for their antiadipogenic effect in 3T3-L1 adipocytes. One of them, the <I>n</I>-BuOH fraction, effectively reduced lipid accumulation and glucose consumption. In addition, the presence of the <I>n</I>-BuOH fraction in adipocytes suppressed the regulations of adipogenic transcription factors, PPARγ and SREBP1c, and adipogenic specific genes, FABP4, FABP1, FAS, LPL, HSL, and ACS1. Further purification of <I>n</I>-BuOH fractions led to the isolation of six phlorotannins (<B>1</B>–<B>6</B>). The six phlorotannins effectively suppressed triglyceride accumulation. Comparative analysis showed that lipid accumulation in adipocytes was dramatically attenuated in the presence of eckstolonol (<B>4</B>).</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2013/jafcau.2013.61.issue-36/jf401454m/production/images/medium/jf-2013-01454m_0007.gif'></P>

Plant homeodomain finger protein 2 promotes bone formation by demethylating and activating Runx2 for osteoblast differentiation

Kim, Hye-Jin,Park, Jong-Wan,Lee, Kyoung-Hwa,Yoon, Haejin,Shin, Dong Hoon,Ju, Uk-Il,Seok, Seung Hyeok,Lim, Seung Hyeon,Lee, Zang Hee,Kim, Hong-Hee,Chun, Yang-Sook Springer Science and Business Media LLC 2014 Cell research Vol.24 No.10

<P>Plant homeodomain finger protein 2 (PHF2), which contains a plant homeodomain and a Jumonji-C domain, is an epigenetic regulator that demethylates lysine 9 in histone 3 (H3K9me2). On the other hand, runt-related transcription factor 2 (Runx2) plays essential roles in bone development and regeneration. Given previous reports that the PHF2 mutation can cause dwarfism in mice and that PHF2 expression is correlated with that of Runx2 in differentiating thymocytes, we investigated whether PHF2 regulates Runx2-mediated bone formation. Overexpression of PHF2 facilitated bone development in newborn mice, and viral shRNA-mediated knockdown of PHF2 delayed calvarial bone regeneration in adult rats. In primary osteoblasts and C2C12 precursor cells, PHF2 enhances osteoblast differentiation by demethylating Runx2, while suppressor of variegation 3-9 homolog 1 (SUV39H1) inhibits bone formation by methylating it. The PHF2-Runx2 interaction is mediated by the Jumonji-C and Runt domains of the two proteins, respectively. The interaction between Runx2 and osteocalcin promoter is regulated by the methylation status of Runx2, i.e., the interaction is augmented when Runx2 is demethylated. Our results suggest that SUV39H1 and PHF2 reciprocally regulate osteoblast differentiation by modulating Runx2-driven transcription at the post-translational level. This study may provide a theoretical basis for the development of new therapeutic modalities for patients with impaired bone development or delayed fracture healing.</P>

Anchored protease-activatable polymersomes for molecular diagnostics of metastatic cancer cells

Kim, Hyun-Ouk,Lim, Jong-Woo,Choi, Jihye,Lee, Hwunjae,Son, Hye Young,Kim, Jihye,Park, Geunseon,Chun, Haejin,Song, Daesub,Huh, Yong-Min,Haam, Seungjoo Royal Society of Chemistry 2017 Journal of Materials Chemistry B Vol. No.

<P>Real-time quantitative and qualitative analyses of metastasis-associated proteases are critical for precise diagnosis and novel therapeutic treatment of advanced cancers. However, conventional methods based on DNA, peptides, and proteins require sophisticated chemistry and additional processes to expose detection moieties, and they lack elements of temporal control, which limit their applicability. We designed unique protease-activatable polymersomes (PeptiSomes) for high sensitivity,<I>in situ</I>quantitative analysis of activating membrane-type 1 matrix metalloproteinases (MT1-MMP, MMP14). To do this, we first synthesized an amphiphilic block polymer-peptide and a copolypeptide based on mPEG-<I>b</I>-pLeu and MT1-peptide-<I>b</I>-pLeu, respectively. Amphiphilic self-assembled PeptiSomes in water were capable of disassembling and releasing the encapsulated self-quenched fluorescence dye (calcein)<I>via</I>enzymatic activation by MT1-MMP. Our PeptiSome system may potentially prevent the initiation and progression of cancer metastasis. Furthermore, the PeptiSome approach described here is likely to facilitate the development of rapid protease assay techniques and further extend the role of proteases as metastasis indicators and therapeutic targets.</P>

Comparison of Growth Charateristics and Protein Analysis in Rice with Barnyard Grass

Haejin Kim,Jihoon Park,Seonghak Lee,Sunhee Woo,Chulwon Lee 한국잡초학회 2010 한국잡초학회 별책(학술대회 초록집) Vol.30 No.2

High Involvement Work Practices and Temporary Employment Practices

Haejin Kim(김혜진) 한국노동연구원 2010 노동정책연구 Vol.10 No.2

본 연구는 미국 뉴저지 주의 소재 병원들에 종사하는 간호사들의 설문조사를 통해, 고성과(고참여)작업관행과 임시고용관행이 노동자들에게 어떤 영향을 미치는지 고찰했다. 이를 위해 임의적 노력이라는 개념이 개발되었는데, 이는 요소 분석을 통해 더 열심히 일하기, 더 현명하게 일하기와 더 친절하게 일하기라는 세 가지로 분류되었다. 고성과(고참여)작업관행은 이 중 더 현명하게 일하기로 부분적으로 매개되어 돌봄 서비스 질 인식과 긍정적 관계를 보여주었으며, 임시고용관행은 더 열심히 일하기로 부분적으로 매개되어 돌봄 서비스 질 인식과 부정적 관계를 보여주었다. 이 연구는 이렇게 상이한 상관관계를 가진 고성과(고참여)작업관행과 임시고용관행을 동시에 연구함으로써 현재 많이 사용되고 있는 이들 관행들의 사용을 재고해 볼 필요를 제기한다. This paper examines how two popular employment practices, high involvement work practices (HIWPs) and temporary employment practices, work on workers and firms, using a survey of nurses in New Jersey, U.S.A., hospitals. For this purpose, discretionary work effort construct is developed, resulting in three factors ? working harder, working smarter, and working kinder effort. Then, a positive relationship is found between HIWPs and perceived quality of work, partially mediated by working smarter effort, and a negative relationship is found between temporary employment practices and perceived quality of work through partial mediation by working harder effort. The simultaneous investigation of HIWPs and temporary employment practices, with these different relationships, gives a chance to reconsider on the use of these two employment trends.

Another Look at Affirmative Action : Voices of U.S. Beneficiaries

Haejin Kim(김혜진) 한국노동연구원 2008 노동정책연구 Vol.8 No.2

적극적 고용조치는 평등의 개념을 ‘기회의 평등’에서 ‘결과의 평등’으로 확장하는 주요 정책의 하나로 설계되었다. 여성을 둘러싼 평등 대 차이 논쟁에 비추어 볼 때, 이 정책은 평등주의적 접근에 기반하고 있다. 적극적 고용조치가 비전통적 직업에 보다 많은 여성을 합류시켰다는 점에서는 긍정적으로 평가받고 있지만, 한편으로는 여성의 특성을 무시하고 남성 규범을 따르도록 했다는 비판도 받고 있다. 미국에서 적극적 고용조치를 통해 남성 중심 직업에서 일하게 된 여성들의 경험담을 통해, 이 논문은 다음과 같은 결론을 이끌어낸다. 비전통적 직업에의 여성의 존재 자체가 이들의 평등한 지위를 보장해 주지는 않는다. 그러나 이들 여성들이 모두 남성 규범을 따르는 것도 아니다. 이 여성들은 다양한 생존전략을 구사하는데, 그 중 일부는 남성성과 여성성이라는 기존 질서에 도전한다. 이들 여성들이 어떤 생존전략을 구사하는가에 관계없이 이들은 기존의 성 정체성을 해체하고 재구성하는 과정을 겪는다. 이들의 경험담은 한국에서도 적극적 고용조치가 평등하면서도 차이를 인정하는 노동 현장을 건설하는데 일조할 수 있다는 가능성을 제시한다. Affirmative Action was designed to expand the equality notion from ‘equality of opportunities’ to ‘equality of outcomes.’ Regarding the equality vs. difference debate on women, it is based on the equality side. While Affirmative Action is appraised to enable more women to get into non-traditional occupations, it is also criticized of ignoring women’s traits and making them imitate men. This paper, through voices of women in non-traditional occupations in the U.S., one group of beneficiaries of Affirmative Action, concludes as follows: The existence of women in nontraditional jobs in itself doesn’t guarantee the equal status for these women. However, it does not lead to these women accepting and following male norms, either. For survival, these women use different strategies, some challenging the given distinction between masculinity and femininity. In the process, they deconstruct the masculine and feminine identities and reconstruct them. The voices of Affirmative Action beneficiaries in the U.S. shed light on a potential of Affirmative Action for building “equal and different” workplaces in Korea.

Stereodefined <i>N,O</i>-Acetals: Pd-CatalyzedSynthesis from Homopropargylic Amines and Utility in the FlexibleSynthesis of 2,6-Substituted Piperidines

Kim, Haejin,Rhee, Young Ho American Chemical Society 2012 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.134 No.9

<P>We developed a conceptually new synthetic strategy whichexploitsthe stereochemical information of labile acyclic <I>N,O</I>-acetals. The key to this strategy, chemo- and stereoselective synthesisof <I>N,O</I>-acetals, was achieved by the Pd-catalyzedaddition of sulfonyl-protected homopropargylic amines to alkoxyallene.The <I>N,O</I>-acetals generated in this way were combinedwith Au-catalyzed cycloisomerization to give an access to 2,6-disubstitutedpiperidines with stereochemical flexibility.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/2012/jacsat.2012.134.issue-9/ja2116298/production/images/medium/ja-2011-116298_0002.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ja2116298'>ACS Electronic Supporting Info</A></P>

Managing the strategic fit of national R&D programme portfolios: the case of creative economy policy in Korea

Kim, Jieun,Jo, Haejin,Kim, Yoonjo,Jeon, Jeonghwan Informa UK (Taylor Francis) 2016 Innovation Vol.18 No.2

ChemInform Abstract: Stereodefined N,O‐Acetals: Pd‐Catalyzed Synthesis from Homopropargylic Amines and Utility in the Flexible Synthesis of 2,6‐Substituted Piperidines.

Kim, Haejin,Rhee, Young Ho WILEY‐VCH Verlag 2012 Chem Inform Vol.43 No.31

<P><B>Abstract</B></P><P>A chiral ligand‐directed approach is used to obtain the N,O‐acetals as precursors for the target chiral tetrahydropyridines.</P>

Participation, Employment Status, and Burnout

Haejin Kim(김혜진) 한국고용노사관계학회 2008 産業關係硏究 Vol.18 No.2

이 글은, 미국 병원에 근무하는 353명의 간호사들에 대한 설문조사를 통해, 고용형태의 차이에 따른 종업원 참여 정도와 번아웃의 관계를 조사하였다. 종업원 참여 정도가 높을수 록 번아웃-특히 소진(exhaustion)과 비인간화(depersonalization) 요소들 이 낮을 것이라는 일반적 가설은 이 조사에서 유효한 것으로 검증되었다. 종업원 참여 정도와 번아웃의 이러한 역관계가 풀타임 정규직과 파트타임, 비정규직 노동자 간에 차이가 없을 것이라는 가설 역시 유효하게 검증되었다. 그러나 파트타임이나 비정규직 노동자들의 경우 종업원 참여 정도가 풀타임 정규직에 비해 낮게 나타났다. 이 연구의 주요 시사점은, 풀타임 정규직만이 아니라 파트타임이나 비정규직 노동자들에게도 종업원 참여의 기회를 주는 것이 기업들에게도 이득이 된다는 사실일 것이다. The relationship among employee participation, employment status, and burnout was examined using a survey of 353 hospital nurses. The hypothesis that employee participation was inversely related to burnout (exhaustion and depersonalization) was supported. The other hypothesis on the same inverse relationship between employee participation and burnout for regular full-time, part-time, and temporary employees was also supported, though participation levels among part-time and temporary nurses were reported lower. The major implication of this study is that organizations can benefit by providing participation opportunities for part-time and temporary as well as full-time employees.