Expression and Prognostic Implications of FOXO3a and Ki67 in Lung Adenocarcinomas

Liu, Hong-Bin,Gao, Xiang-Xiang,Zhang, Qing,Liu, Jian,Cui, Yuan,Zhu, Yan,Liu, Yi-Fei Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.4

To investigate the significance of FOXO3a and Ki67 in human lung adenocarcinomas. Envision immunohistochemical staining and Western blotting were used to examine the protein expression of FOXO3a in 127 cases of human lung adenocarcinoma specimens. The positive rate in lung adenocarcinoma (55.9%) was lower than that in normal tissues (80%). We found that the expression of FOXO3a was closely related with the degree of differentiation, TNM staging, lymph node metastasis and survival. In addition, significant differences in the different pathological types of lung adenocarcinoma cases (P<0.01). The FOXO3a positive rate of the acini as the main type (APA) (86.7%) and the lepidic as the main type (LPA) (82.4%) was higher than the solid as the main type (SPA) (50.0%), the papilla as the main type (PPA) (42.9%) and the micropapilla as the main type (MPA) (9.4%). Moreover, the expression of FOXO3a was negatively related with Ki67 expression. Our results suggested that the expression of FOXO3a is closely correlated with the aggressiveness of lung adenocarcinoma. It was indicated that disregulation of FOXO3a might play key roles in the occurrence and development of lung a denocarcinoma and joint detection of the two markers might play an important role in diagnosing tumors.

Cadmium induces apoptosis in primary rat osteoblasts through caspase and mitogen-activated protein kinase pathways

Hong-Yan Zhao,Wei Liu,Yi Wang,Nannan Dai,Jian-Hong Gu,Yan Yuan,Xue-Zhong Liu,Jian-Chun Bian,Zong-Ping Liu 대한수의학회 2015 Journal of Veterinary Science Vol.16 No.3

Exposure to cadmium (Cd) induces apoptosis in osteoblasts (OBs); however, little information is available regarding the specific mechanismsof Cd-induced primary rat OB apoptosis. In this study, Cd reduced cell viability, damaged cell membranes and induced apoptosis in OBs. We observed decreased mitochondrial transmembrane potentials, ultrastructure collapse, enhanced caspase-3 activity, and increasedconcentrations of cleaved PARP, cleaved caspase-9 and cleaved caspase-3 following Cd treatment. Cd also increased the phosphorylationof p38-mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinases (ERK)1/2 and c-jun N-terminal kinase (JNK) in OBs. Pretreatment with the caspase inhibitor, N-benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone, ERK1/2 inhibitor (U0126), p38 inhibitor(SB203580) and JNK inhibitor (SP600125) abrogated Cd-induced cell apoptosis. Furthermore, Cd-treated OBs exhibited signs of oxidativestress protection, including increased antioxidant enzymes superoxide dismutase and glutathione reductase levels and decreased formationof reactive oxygen species. Taken together, the results of our study clarified that Cd has direct cytotoxic effects on OBs, which are mediatedby caspase- and MAPK pathways in Cd-induced apoptosis of OBs.

A FEEDBACK-DRIVEN BUBBLE G24.136+00.436: A POSSIBLE SITE OF TRIGGERED STAR FORMATION

Liu, Hong-Li,Wu, Yuefang,Li, JinZeng,Yuan, Jing-Hua,Liu, Tie,Dong, Xiaoyi IOP Publishing 2015 The Astrophysical journal Vol.798 No.1

<P>We present a multi-wavelength study of the IR bubble G24.136+00.436. The J = 1-0 observations of (CO)-C-12, (CO)-C-13, and (CO)-O-18 were carried out with the Purple Mountain Observatory 13.7 m telescope. Molecular gas with a velocity of 94.8 km s(-1) is found prominently in the southeast of the bubble, shaped as a shell with a total mass of similar to 2 x 10(4) M-circle dot. It was likely assembled during the expansion of the bubble. The expanding shell consists of six dense cores, whose dense (a few of 10(3) cm(-3)) and massive (a few of 10(3) M-circle dot) characteristics coupled with the broad linewidths (>2.5 km s(-1)) suggest that they are promising sites for forming high-mass stars or clusters. This could be further consolidated by the detection of compact H II regions in Cores A and E. We tentatively identified and classified 63 candidate young stellar objects (YSOs) based on the Spitzer and UKIDSS data. They are found to be dominantly distributed in regions with strong molecular gas emission, indicative of active star formation, especially in the shell. The H II region inside the bubble is mainly ionized by a similar to O8V star(s), of the dynamical age of similar to 1.6 Myr. The enhanced number of candidate YSOs and secondary star formation in the shell as well as the timescales involved, indicate a possible scenario for triggering star formation, signified by the 'collect and collapse' process.</P>

Emulsion-directed liquid/liquid interfacial fabrication of lanthanide ion-doped block copolymer composite thin films

Hong, Ming,Geng, Yuanyuan,Liu, Mei,Xu, Yuan,Lee, Yong-Ill,Hao, Jingcheng,Liu, Hong-Guo Elsevier 2015 JOURNAL OF COLLOID AND INTERFACE SCIENCE - Vol.438 No.-

<P><B>Abstract</B></P> <P>An emulsion-directed assembly and adsorption approach has been used to fabricate composite films of polystyrene-b-poly(acryl acid)-b-polystyrene (PS-b-PAA-b-PS) and Eu<SUP>3+</SUP> and La<SUP>3+</SUP> ions at the planar liquid/liquid interface of the polymer DMF/chloroform (1:1, v/v) mixed solution (lower phase) and aqueous solutions of the corresponding salts (upper phase). The lower phase gradually transformed to a water-in-oil (W/O) emulsion via spontaneous emulsification due to the “ouzo effect”. Polymer molecules and the metal ions assembled around emulsion droplets that adsorbed at the planar liquid/liquid interface at last, resulting in formation of composite films. The film morphologies and structures depend on Ln<SUP>3+</SUP> ions: polymer/Eu<SUP>3+</SUP> composite films were foam films composed of microcapsules ranging in size from several hundreds of nanometers to micrometers, while polymer/La<SUP>3+</SUP> composite films were composed of hollow spheres several tens of nanometers in size. Fourier transform infrared (FTIR) spectra revealed that the coordination modes of carboxyl groups to Eu<SUP>3+</SUP> and La<SUP>3+</SUP> were bridging bidentate and ionic, respectively, in the two types of composites. These results indicate that stable microcapsules can be fabricated around droplets for polymer/Eu<SUP>3+</SUP> systems, while microcapsules of polymer/La<SUP>3+</SUP> are unstable. This leads to different film morphologies and structures. Compositions of these films were characterized using energy-dispersive spectroscopy (EDS) and X-ray photoelectron spectroscopy (XPS). In addition, foam films of polymer/Eu<SUP>3+</SUP>/2,2′-bipyridine (bpy) were fabricated using this approach, and their photoluminescence properties were investigated.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Composite films of block copolymer/Ln<SUP>3+</SUP> were fabricated at liquid/liquid interfaces. </LI> <LI> Eu<SUP>3+</SUP> and La<SUP>3+</SUP> have great effects on morphologies and microstructures of the films. </LI> <LI> Polymer/Eu<SUP>3+</SUP> and polymer/Eu<SUP>3+</SUP>/bpy films exhibit good luminescent properties. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Solanum Nigrum Polysaccharide (SNL) Extract Effects in Transplanted Tumor-bearing Mice - Erythrocyte Membrane Fluidity and Blocking of Functions

Yuan, Hong-Liang,Liu, Xiao-Lei,Liu, Ying-Jie Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.23

Background: Solanum nigrum L. has been used in traditional Chinese medicine because of its diuretic and antipyretic effects. The present research concerned effects of crude polysaccharides isolated from Solanum nigrum L. on erythrocyte membranes of tumor-bearing $S_{180}$ and $H_{22}$ in mice. Materials and Methods: Fluorescence-labeled red blood cell membranes were used with DPH fluorescence spectrophotometry to examine erythrocyte membrane fluidity, and colorimetry to determine degree of erythrocyte surface membrane blocking. Extent of reaction by tumor-bearing mice with the enzyme erythrocyte membrane bubble shadow detection of red cell membrane variation in the degree of closure before and after administration. Results: Solanum nigrum polysaccharide could significantly improve the $S_{180}$ and $H_{22}$ tumor-bearing mice erythrocyte membrane fluidity, compared with the control group, the difference was significant (p<0.01), SNL can significantly improve the red blood cell membrane and then $S_{180}$ tumor-bearing mice sealing ability, compared with the negative control group, the difference was significant(p<0.05, p<0.01). $H_{22}$ tumor-bearing mice can increase red cell membrane and then sealing ability, the difference was significant (p<0.05). Solanum nigrum polysaccharide degree of fluidity and blocking two transplanted tumors in mice restored the ability to raise the red cell membrane has a significant effect. Conclusions: Solanum nigrum L.-type mice transplanted tumor can affect the red blood cell membrane fluidity and re-closed, through the red cell membrane of red blood cells to enhance the immune function of the possibility of erythrocyte immunity against tumor formation garland provide experimental basis.

Effects of Ribosomal Protein L39-L on the Drug Resistance Mechanisms of Lung Cancer A549 Cells

Liu, Hong-Sheng,Tan, Wen-Bin,Yang, Ning,Yang, Yuan-Yuan,Cheng, Peng,Liu, Li-Juan,Wang, Wei-Jie,Zhu, Chang-Liang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.7

Background: Cancer is a major threat to the public health whether in developed or in developing countries. As the most common primary malignant tumor, the morbidity and mortality rate of lung cancer continues to rise in recent ten years worldwide. Chemotherapy is one of the main methods in the treatment of lung cancer, but this is hampered by chemotherapy drug resistance, especially MDR. As a component of the 60S large ribosomal subunit, ribosomal protein L39-L gene was reported to be expressed specifically in the human testis and human cancer samples of various tissue origins. Materials and Methods: Total RNA of cultured drug-resistant and susceptible A549 cells was isolated, and real time quantitative RT-PCR were used to indicate the transcribe difference between amycin resistant and susceptible strain of A549 cells. Viability assay were used to show the amycin resistance difference in RPL39-L transfected A549 cell line than control vector and null-transfected A549 cell line. Results: The ribosomal protein L39-L transcription level was 8.2 times higher in drug-resistant human lung cancer A549 cell line than in susceptible A549 cell line by quantitative RT-PCR analysis. The ribosomal protein L39-L transfected cells showed enhanced drug resistance compared to plasmid vector-transfected or null-transfected cells as determined by methyl tritiated thymidine (3H-TdR) incorporation. Conclusions and Implications for Practice: The ribosomal protein L39-L gene may have effects on the drug resistance mechanism of lung cancer A549 cells.

INTERACTIONS OF THE INFRARED BUBBLE N4 WITH ITS SURROUNDINGS

Liu, Hong-Li,Li, Jin-Zeng,Wu, Yuefang,Yuan, Jing-Hua,Liu, Tie,Dubner, G.,Paron, S.,Ortega, M. E.,Molinari, Sergio,Huang, Maohai,Zavagno, Annie,Samal, Manash R.,Huang, Ya-Fang,Zhang, Si-Ju American Astronomical Society 2016 The Astrophysical journal Vol.818 No.1

<P>The physical mechanisms that induce the transformation of a certain mass of gas in new stars are far from being well understood. Infrared bubbles associated with H II regions have been considered to be good samples for investigating triggered star formation. In this paper we report on the investigation of the dust properties of the infrared bubble N4 around the H II. region G11.898+0.747, analyzing its interaction with its surroundings and star formation histories therein, with the aim of determining the possibility of star formation triggered by the expansion of the bubble. Using Herschel PACS and SPIRE images with a wide wavelength coverage, we reveal the dust properties over the entire bubble. Meanwhile, we are able to identify six dust clumps surrounding the bubble, with a mean size of 0.50 pc, temperature of about 22 K, mean column density of 1.7 x 10(22) cm(-2), mean volume density of about 4.4 x 10(4) cm(-3), and a mean mass of 320M(circle dot). In addition, from PAH emission seen at 8 mu m, free-free emission detected at 20 cm, and a probability density function in special regions, we could identify clear signatures of the influence of the H II region on the surroundings. There are hints of star formation, though further investigation is required to demonstrate that N4 is the triggering source.</P>

Current Evidence on the Association between rs3757318 of C6orf97 and Breast Cancer Risk: a Meta-Analysis

Hong, Yuan,Chen, Xue-Qin,Li, Jiao-Yuan,Liu, Cheng,Shen, Na,Zhu, Bei-Bei,Gong, Jing,Chen, Wei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19

Background: A common genetic variant rs3757318, located in intron of C6orf97, was firstly identified to be associated with breast cancer (BC) risk by a genome-wide association (GWA) study. However, subsequent validation studies with different ethnicities have yielded conflicting results. Materials and Methods: We performed a meta-analysis to synthesize all available data for evaluating the precise effect of this variant on BC susceptibility. Results: A total of 8 articles containing 11 studies with 62,891 cases and 65,635 controls were included in this meta-analysis. When compared to the G allele, the rs3757318-A allele was significantly associated with BC risk with the pooled OR of 1.21 (95% CI=1.15 - 1.29, P<0.001) but with obvious between-study heterogeneity (P=0.040). Stratified analysis suggested that diversity of ethnicity along with control source may explain part of the heterogeneity. Similarly, significant associations were also identified in heterozygote, homozygote, dominant and recessive genetic models. Sensitivity and publication bias analyses indicated robust stability of our results. Conclusions: Our present meta-analysis demonstrated that the variant rs3757318 is associated with increased BC risk. Nevertheless, further studies are needed to clarify the underlying biological mechanisms.

A preparation and performance study of glass-ceramic glazes derived from blast furnace slag and fly ash

Hong-xia Lu,Man He,Yuan-yuan Liu,Jing-fei Guo,Li-wei Zhang,Deliang Chen,Hai-long Wang,Hong-liang Xu,Rui Zhang 한양대학교 세라믹연구소 2011 Journal of Ceramic Processing Research Vol.12 No.5

Glass-ceramic glazes have been prepared successfully via crystallization from blast-furnace slag (BFS), fly ash (FA) fluxed with potash feldspar and borax. The crystalline behavior of glass-ceramic glazes was investigated using differential thermal analysis and thermogravimetric analysis (DTA/TG), X-ray diffraction (XRD) and scanning electron microscopy (SEM). Results revealed that the major crystalline phases are anorthite (CaAl2Si2O8) and akermanite (Ca2MgSi2O7) and crystalline phases disperse well in glassy phases with a uniform size of 1 μm. Glass-ceramic glazes possess low density, low water absorption,perfect stain resistance, acid resistance and alkali resistance. The thermal expansion coefficient of glass-ceramic glazes is steady up to 800 oC with an average value of 7.2 × 10−6 /K. Final results suggest that BFS and FA have potential to be vitrified into economically and environmentally low-cost glass-ceramic glaze materials.

Statistical Optimization of Culture Media and Conditions for Production of Mannan by Saccharomyces cerevisiae

Hong-Zhi Liu,Qiang Wang,Yuan-Yuan Liu,Fang Fang 한국생물공학회 2009 Biotechnology and Bioprocess Engineering Vol.14 No.5

In view of the increase in Saccharomyces cerevisiae mannan content, the culture medium and condition for S.cerevisiae were optimized in this study. The influence of culture medium ingredients such as carbon and nitrogen sources, inorganic ion, and enzyme activator on mannan production were evaluated using factional design. The mathematical model was established by the quadratic rotary combination design through response surface analysis. The optimized concentrations of culture medium were determined as follows: 4.98 g/100 mL, sucrose; 4.39 g/100 mL, soybean peptone; 3.10 g/100 mL, yeast extract; and 2.21 g/100 mL, glycerol. The optimized culture medium increased mannan production from 82.7 ± 3.4 mg/100 mL to 162.53 ± 3.47 mg/100 mL. The influence of original pH, inoculum size, temperature, and media volume on mannan production was evaluated and confirmed by orthogonale experimental design, with the order of effect as follows: media volume > temperature > initial pH > inoculation size. The optimized culture condition was pH, 5; inoculum size, 5 ml; temperature, 32oC; and media volume, 40 mL. The maximum mannan production increased to 258.5 ± 9.1 mg/100 mL at the optimum culture condition.=It was evident that the mannan production was affected significantly by culture medium and condition optimization (é < 0.01). In view of the increase in Saccharomyces cerevisiae mannan content, the culture medium and condition for S.cerevisiae were optimized in this study. The influence of culture medium ingredients such as carbon and nitrogen sources, inorganic ion, and enzyme activator on mannan production were evaluated using factional design. The mathematical model was established by the quadratic rotary combination design through response surface analysis. The optimized concentrations of culture medium were determined as follows: 4.98 g/100 mL, sucrose; 4.39 g/100 mL, soybean peptone; 3.10 g/100 mL, yeast extract; and 2.21 g/100 mL, glycerol. The optimized culture medium increased mannan production from 82.7 ± 3.4 mg/100 mL to 162.53 ± 3.47 mg/100 mL. The influence of original pH, inoculum size, temperature, and media volume on mannan production was evaluated and confirmed by orthogonale experimental design, with the order of effect as follows: media volume > temperature > initial pH > inoculation size. The optimized culture condition was pH, 5; inoculum size, 5 ml; temperature, 32oC; and media volume, 40 mL. The maximum mannan production increased to 258.5 ± 9.1 mg/100 mL at the optimum culture condition.=It was evident that the mannan production was affected significantly by culture medium and condition optimization (é < 0.01).