Global Histone H4 Acetylation of IGF1 and GH Genes in Lungs of Somatic Cell Cloned Calves

Zhang, L.,Wang, S.H.,Fan, B.L.,Dai, Y.P.,Fei, J.,Li, N. Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.8

Histone acetylation modification is one key mechanism in the regulation of gene activation. In this study, we investigated the global levels of histone H4 acetylation of insulin like growth factor I (IGF1) and growth hormone (GH) genes in the lungs of two somatic cell cloned calves. Data showed the levels of histone H4 acetylation of IGF1 and GH genes vary widely within different gene regions, and, in almost all regions of the two genes, acetylation levels are lower in the aberrant clone than in the normal clone. Thus we suggest that inefficient epigenetic reprogramming in the clone may affect the balance between acetylation and deacetylation, which will affect normal growth and development. These findings will also have implications for improvement of cloning success rates.

Broadband omnidirectional light detection in flexible and hierarchical ZnO/Si heterojunction photodiodes

Lim, S.,Um, D. S.,Ha, M.,Zhang, Q.,Lee, Y.,Lin, Y.,Fan, Z.,Ko, H. Springer Science + Business Media 2017 Nano research Vol.10 No.1

<P>The development of flexible photodetectors has received great attention for future optoelectronic applications including flexible image sensors, biomedical imaging, and smart, wearable systems. Previously, omnidirectional photodetectors were only achievable by integration of a hemispherical microlens assembly on multiple photodetectors. Herein, a hierarchical photodiode design of ZnO nanowires (NWs) on honeycomb-structured Si (H-Si) membranes is demonstrated to exhibit excellent omnidirectional light-absorption ability and thus maintain high photocurrents over broad spectral ranges (365 to 1,100 nm) for wide incident angles (0A degrees to 70A degrees), which enabled broadband omnidirectional light detection in flexible photodetectors. Furthermore, the stress-relieving honeycomb pattern within the photodiode micromembranes provided photodetectors with excellent mechanical flexibility (10% decrease in photocurrent at a bending radius of 3 mm) and durability (minimal change in photocurrent over 10,000 bending cycles). When employed in semiconductor thin films, the hierarchical NW/honeycomb heterostructure design acts as an efficient platform for various optoelectronic devices requiring mechanical flexibility and broadband omnidirectional light detection.</P>

Prevalence and outcomes of acute respiratory distress syndrome in patients with aneurysmal subarachnoid hemorrhage: a systematic review and meta-analysis

Fan Tracey H.,Huang Merry,Price Carrie,Premraj Lavienraj,Kannapadi Nivedha V.,Suarez Jose I.,Cho Sung-Min 대한신경집중치료학회 2022 대한신경집중치료학회지 Vol.15 No.1

Background: In this study, we aimed to investigate the prevalence, timing, risk factors, and outcomes of acute respiratory distress syndrome (ARDS) in patients with aneurysmal subarachnoid hemorrhage (aSAH). Methods: PubMed and four other databases were searched for randomized controlled trials (RCTs) and observational studies of patients 18 years or older through October 20, 2021. Study quality was assessed, using the Cochrane Risk of Bias tool for RCTs and the Newcastle-Ottawa Scale for cohort and case-control studies. High-grade aSAH was defined as a Hunt-Hess grade≥3 and/or a modified Fisher score≥3. A good neurological outcome was defined as a Glasgow Outcome Scale score ≥4. Random-effects meta-analyses were conducted to estimate the pooled outcome prevalence and 95% confidence interval (CI). Results: Eleven observational studies (n=6,107) met the inclusion criteria. Overall, 15% of the patients (95% CI=10.5–20.0; I2=97.8%) developed ARDS after aSAH, with a mean time of 3 days (95% CI=1.9–3.7; I2=54%). Overall survival at discharge was 80% (95% CI=75–86; I2=96%), and 67% of aSAH patients (95% CI=54.9–78.9; I2=94%) had a good neurological outcome at any time. The aSAH cohort without ARDS had a higher rate of survival than those with ARDS (79% vs. 49%, P=0.028). Male sex, patients with a high-grade aSAH, patients who developed pneumonia, and systemic inflammatory response syndrome during hospital admission were at a higher risk of developing ARDS.Conclusion: In this meta-analysis, approximately one in six patients developed ARDS after aSAH, with a mean time of 3 days from the initial presentation, and ARDS was associated with increased mortality.

Buckling of axial compressed cylindrical shells with stepwise variable thickness

H.G. Fan,Z.P. Chen,W.Z. Feng,F. Zhou,X.L. Shen,G.W. Cao 국제구조공학회 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.54 No.1

This paper focuses on an analytical research on the critical buckling load of cylindrical shells with stepwise variable wall thickness under axial compression. An arctan function is established to describe the thickness variation along the axial direction of this kind of cylindrical shells accurately. By using the methods of separation of variables, small parameter perturbation and Fourier series expansion, analytical formulas of the critical buckling load of cylindrical shells with arbitrary axisymmetric thickness variation under axial compression are derived. The analysis is based on the thin shell theory. Analytic results show that the critical buckling load of the uniform shell with constant thickness obtained from this paper is identical with the classical solution. Two important cases of thickness variation pattern are also investigated with these analytical formulas and the results coincide well with those obtained from other authors. The cylindrical shells with stepwise variable wall thickness, which are widely used in actual engineering, are studied by this method and the analytical formulas of critical buckling load under axial compression are obtained. Furthermore, an example is presented to illustrate the effects of each strake’s length and thickness on the critical buckling load.

Analysis of a preliminary configuration for a floating wind turbine

H.F. Wang,Y.H. Fan,Iñigo Moreno 국제구조공학회 2016 Structural Engineering and Mechanics, An Int'l Jou Vol.59 No.3

There are many theoretical analyses and experimental studies of the hydrodynamics for the tension leg platform (TLP) of a floating wind turbine. However, there has been little research on the arrangement of the TLP’s internal structure. In this study, a TLP model and a 5-MW wind turbine model as proposed by the Minstitute of Technology and the National Renewable Energy Laboratory have been adopted, respectively, to comprehensively analyze wind effects and wave and current combinations. The external additional coupling loads on the TLP and the effects of the loads on variables of the internal structure have been calculated. The study investigates preliminary layout parameters-namely, the thickness of the tension leg body, the contact mode of the top tower on the tension leg, the internal stiffening arrangement, and the formation of the spoke structure-and conducts sensitivity analyses of the TLP internal structure. Stress is found to be at a maximum at the top of the tension leg structure and the maximum stress has low sensitivity to the load application point. Different methods of reducing maximum stress have been researched and analyzed, and the effectiveness of these methods is analyzed. Filling of the spoke structure with concrete is discussed. Since the TLP structure for offshore wind power is still under early exploration, arrangements and the configuration of the internal structure, exploration and improvements are ongoing. With regard to its research and analysis process, this paper aims to guide future applications of tension leg structures for floating wind turbine.

Buckling of axial compressed cylindrical shells with stepwise variable thickness

Fan, H.G.,Chen, Z.P.,Feng, W.Z.,Zhou, F.,Shen, X.L.,Cao, G.W. Techno-Press 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.54 No.1

This paper focuses on an analytical research on the critical buckling load of cylindrical shells with stepwise variable wall thickness under axial compression. An arctan function is established to describe the thickness variation along the axial direction of this kind of cylindrical shells accurately. By using the methods of separation of variables, small parameter perturbation and Fourier series expansion, analytical formulas of the critical buckling load of cylindrical shells with arbitrary axisymmetric thickness variation under axial compression are derived. The analysis is based on the thin shell theory. Analytic results show that the critical buckling load of the uniform shell with constant thickness obtained from this paper is identical with the classical solution. Two important cases of thickness variation pattern are also investigated with these analytical formulas and the results coincide well with those obtained from other authors. The cylindrical shells with stepwise variable wall thickness, which are widely used in actual engineering, are studied by this method and the analytical formulas of critical buckling load under axial compression are obtained. Furthermore, an example is presented to illustrate the effects of each strake's length and thickness on the critical buckling load.

Studies on Filariasis in Kinmen (Quemoy) Islands, Republic of China

Fan,P.C.,Wang,Y.C.,Liu,J.C.,Lo,H.S.,Hsu,Y.P.,Yen,C.H.,Lin,C.C.,Hsu,S.T. INSTITUTE OF TROPICAL MEDICINE YONSEI UNIVERSITY 1974 YONSEI REPORTS ON TROPICAL MEDICINE Vol.5 No.1

1972年 7月 1日부터 1973年 6月 30日까지 1年間 中華民國 臺?省 金門鳥에서 絲狀 症에 關한 調査硏究를 實施한 바 다음과 같은 結果를 얻었다. 1. 金門鳥 55村落에 居住하는 20,018 住民을 對象으로 調査한 바 血中 microfilariae (mf.) 兩性率은 8.8%였고 血液 20立方mm當 mf농도는 16.2였으며 絲狀 感染으로 因 臨床例 는 19.2%였다. 金門鳥 駐屯 軍人 2,428名에서 mf.양성율은 0.1%, mf. 감염농도는 20立方mm當 26.3이었으며 臨床例는 없었다. mf.感染濃度 및 臨床例에 있어 확실히 女性보다는 男性에서 高率을 보였으나 mf.陽性率은 兩性間에 差異가 없었다. mf.陽性率과 臨床例는 年齡의 增加와 一致하였으나 mf. 감렴농도는 年齡의 증가와 無關하였다. 2. 金文鳥 駐屯 軍人에 있어 주둔 其間과 絲狀 感染과는 關係가 없었으며 駐屯基地와 村落과의 距離와도 無關하였다. 軍人들은 모기에 물리지 않도록 잘 保護되어 있었고 주둔지역은 完全히 住民들과 격리되어 있었다. 3. 51村落으로부터의 血液檢査를 받은 1,470名의 絲狀 感染陽性者 中에서 臨床病勢르 보인 例는 19.2%였으며 女性보다는 男性에서 約 5倍가 많았다. 男性에 있어서의 大部分의 臨床例는 生殖器에 症狀을 보였는데 其中 10%는 象皮症을 가진 例는 2名뿐이었다. 4. 43村落의 家屋內에서 잡힌 모기는 4屬 14種(Culex 10種, Aedes 2種, Anopheles 1種, Armigeres 1種)이었으며 이들中 Cules P. fatigans와 Armigeres subalbatus가 大部分이었으나 其他 12種은 매우 적었다. Culcx mimesticus, C. (L) vorax, C. fuscocephala 및 Acdes nocturnus등 의 4新種은 처음으로 發見되었다. Culex p.fatigans는 現存「반크롭트」 絲狀 만을 옮기는 媒介昆 으로 알려져 있다. 治療前 8個 村落으로부터 잡은 438마리의 Culex p. fatigans에서 幼 을 內包하고 있는 모기는 1마리 平均 5.9마리의 絲狀 幼 을 갖고 있는 셈이었으며 全體 感染率은 11.9%, 感染期 幼 을 가진 모기는 10.5%이었다. 上記 8個 村落 住民을 治癒한 後의 樣狀은 治療前과 아주 달라서 모기 1마리當 平均 2.1마리의 幼 을 갖고 있었고 全體感染率은 2.2%, 感染期幼 內包率은 0.09%였다. 平均 pH가 7.4(6.4∼8.0)인 8個處의 모기 産卵場所에서 잡힌 모기 幼 은 모두 11種이었다. 有機燐劑의 一種인 Sumithion을 1平方m當 0.2gm의 농도로 모기 産卵場所 水面에 撒布하였던바 모든 모기 幼 에 對한 殺 果가 좋았다. 5. 總 1.590名의 絲狀 感染者에게 成人當 總量 5gm의 diethylcarbamazine을 10∼12日 間 服用시켜 보았다. 治療率은 80.6%, mf. 減少率은 96.9%였고 反應率는 67.2%였다. 여러 가지 副作用은 있었으나 一般的으로 輕微하였으며 患者가 참을 수 있었으므로 最高用量으로 投藥했을 경우에도 大部分 거절 하지 않았다. 8個 部落에서 捕獲된 Culex P. fatigans의 絲狀 幼 全體感染率, 感染期幼 內包率 및 感染濃度는 患者治療前에 各各 11.9%, 10.5%, 5.9였는데 治療後에는 2.2%, 0.09%, 2.1로 떨어져서 diethylcarbamazine은 microfilariae에 對한 殺충효果가 높을 뿐 아니라 絲狀충 만延을 組止시키는데 좋은 藥劑라고 思料된다 The present investigation is a serial study on “Filariasis in Kinmen (Quemoy) Islands”, which was performed from July 1, 1972 to June 30, 1973. The results are summarized as follows; 1. Parasitological investigation: Of 20,018 Kinmen Chinese in 55 villages examined, the microfilariae (mf.) rate of 8.8%, mf. density of 16.2 per 20cmm blood and clinical rate 19.2% were found: the corresponding figures for a total of 2,428 military personnel were 0.1%, 29.3 and 0.0% respectively. The mf. density and clinical rate were obviously higher in the males than in the females, but no difference was found for the microfilarial rate. The mf. rate and clinical rate were correspondingly increased with age, but there was no difference in mf. density. 2. Transmission of filariasis: It seems no any relation to the length of stationing of the Chinese troops on Kinmen Islands, and also no relation to the distance between the villages and camps; because the servicemen are well protected from the mosquito bites and bites and the camps are completely isolated, to and off limit to the villagers, though they have common places of entertainment. 3. Clinical investigation: Of 1,470 filarial carriers in 51 villages examined physically, the clinical rate of 19.2% was detected. It was about 5 times higher in males than in females. In the males, most of the cases the trouble in their reproductive organs, and 10% had elephantiasis; but in the females, the lymph nodes enlargement and chyluria were more common, only 2 cases had elephantiasis. The clinical rates increased corresponding with age. 4. Entomological investigation: Fourteen(14) species of 4 genera (10 Culex, 2 Aedes, 1 Anopheles and 1 Armigeres) of household mosquitoes were collected from indoor resting sites of 43 villages on kinmen Islands, in which, Cules P. fatigans and Armigeres subalbatus were the most common species, but the remaining 12 species are faily rare. Four (4) new species: Culex mimeticus, C. (L) vorax, C, fucocephala and Aedes noturnvs were found for the first time. Culex p.fatigans is still the only vector. in transmission of bancroftian filariasis. The infection rate of 11.9%, infective rate of 10.5% and 5.9 filarial larvae per infected mosquito were detected in 438 Culex p. fatigans from 8 villages before treatment. The corresponding figures were much lower in the above same villages after treatment (2.2%, 0.09% and 2.1). Eleven (11) species of moswquito larvae were collected from 8 kinds of breeding sites; where, the pH value of 7.4(ranged 6.4∼8.0) was measured. “Sumithion”is one of the organophosphorous compounds, which was first used on the water surface with the dosage of 0.2gm per square meter.A high insecticidal effect against different species of mosquito larvae was observed. 5. Chemotherapy of filariasis: Of 1.590 filarial carriers scheme, with a total does of 5 gm of diethylcarbamazine per adult case for a period of 10∼12 days schedule was performed. The cure rate of 80.6%, mf. reduction tate of 96.9% and reaction rate of 67.2% were found. Although many kinds of side effects were encountered, they were usually mild or transient and could be tolerated by the patients, thus there was almost no refused to the administration of the drug even if the drug was increased to the maximum dosage. The natural infection rate, infective rate and filarial larval density of Culex P. fatigans in 8 villages were found much higher before control measure (11.9%, 10.5% and 5.9%) than after control (2.2%, 0.09% and 2.1). It showed that diethylcarbamazine is a powerful microfilaricidal drug in eliminating the filarial infection and reducing this transmission.

Effect of Age on Energy Requirement for Maintenance and Growth of Dorper and Hu Crossbred F1 Ewes Weighing 20 to 50 kg

H.T. Nie,Y.J. Wan,J.H. You,Z.Y. Wang,S. Lan,Y.X. Fan,F. Wang 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.8

This research aimed to define the energy requirement of Dorper and Hu Hybrid F1 ewes 20 to 50 kg of body weight, furthermore to study energy requirement changes with age and evaluate the effect of age on energy requirement parameters. In comparative slaughter trial, thirty animals were divided into three dry matter intake treatments (ad libitum, n = 18; low restricted, n = 6; high restricted, n = 6), and were all slaughtered as baseline, intermediate, and final slaughter groups, to calculate body chemical components and energy retained. In digestibility trial, twelve ewes were housed in individual metabolic cages and randomly assigned to three feeding treatments in accordance with the design of a comparative slaughter trial, to evaluate dietary energetic values at different feed intake levels. The combined data indicated that, with increasing age, the net energy requirement for maintenance (NEm) decreased from 260.62±13.21 to 250.61±11.79 kJ/kg0.75 of shrunk body weight (SBW)/d, and metabolizable energy requirement for maintenance (MEm) decreased from 401.99±20.31 to 371.23±17.47 kJ/kg0.75 of SBW/d. Partial efficiency of ME utilization for maintenance (km, 0.65 vs 0.68) and growth (kg, 0.42 vs 0.41) did not differ (p>0.05) due to age; At the similar condition of average daily gain, net energy requirements for growth (NEg) and metabolizable energy requirements for growth (MEg) for ewes during late fattening period were 23% and 25% greater than corresponding values of ewes during early fattening period. In conclusion, the effect of age upon energy requirement parameters in the present study were similar in tendency with previous recommendations, values of energy requirement for growth (NEg and MEg) for Dorper and Hu crossbred female lambs ranged between the NRC (2007) recommendation for early and later maturating growing sheep.

MODEL-BASED DESIGN OF A VARIABLE NOZZLE TURBOCHARGER CONTROLLER

H. G. ZHANG,E. H. WANG,B. Y. FAN,M. G. OUYANG,S. Z. XIA 한국자동차공학회 2011 International journal of automotive technology Vol.12 No.2

Variable Nozzle Turbocharger (VNT) was invented to solve the problem of matching an ordinary turbocharger with an engine. VNT can harness exhaust energy more efficiently, enhance intake airflow response and reduce engine emissions, especially during transient operating conditions. The difficulty of VNT control lies in how to regulate the position of the nozzle at different engine working conditions. The control strategy designed in this study is a combination of a closedloop feedback controller and an open-loop feed-forward controller. The gain-scheduled proportional-integral-derivative (PID)controller was implemented as the feedback controller to overcome the nonlinear characteristic. As it is difficult to tune the parameters of the gain-scheduled PID controller on an engine test bench, system identification was used to identify the plant model properties at different working points for a WP10 diesel engine on the test bench. The PID controller parameters were calculated based on the identified first-order-plus–dead-time (FOPDT) plant model. The joint simulation of the controller and the plant model was performed in Matlab/Simulink. The time-domain and frequency-domain performances of the entire system were evaluated. The designed VNT control system was verified with engine tests. The results indicated that the real boosting pressure traced the target boosting pressure well at different working conditions.

Effect of Age on Energy Requirement for Maintenance and Growth of Dorper and Hu Crossbred F1 Ewes Weighing 20 to 50 kg

Nie, H.T.,Wan, Y.J.,You, J.H.,Wang, Z.Y.,Lan, S.,Fan, Y.X.,Wang, F. Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.8

This research aimed to define the energy requirement of Dorper and Hu Hybrid $F_1$ ewes 20 to 50 kg of body weight, furthermore to study energy requirement changes with age and evaluate the effect of age on energy requirement parameters. In comparative slaughter trial, thirty animals were divided into three dry matter intake treatments (ad libitum, n = 18; low restricted, n = 6; high restricted, n = 6), and were all slaughtered as baseline, intermediate, and final slaughter groups, to calculate body chemical components and energy retained. In digestibility trial, twelve ewes were housed in individual metabolic cages and randomly assigned to three feeding treatments in accordance with the design of a comparative slaughter trial, to evaluate dietary energetic values at different feed intake levels. The combined data indicated that, with increasing age, the net energy requirement for maintenance ($NE_m$) decreased from $260.62{\pm}13.21$ to $250.61{\pm}11.79kJ/kg^{0.75}$ of shrunk body weight (SBW)/d, and metabolizable energy requirement for maintenance (MEm) decreased from $401.99{\pm}20.31$ to $371.23{\pm}17.47kJ/kg^{0.75}$ of SBW/d. Partial efficiency of ME utilization for maintenance ($k_m$, 0.65 vs 0.68) and growth ($k_g$, 0.42 vs 0.41) did not differ (p>0.05) due to age; At the similar condition of average daily gain, net energy requirements for growth ($NE_g$) and metabolizable energy requirements for growth ($ME_g$) for ewes during late fattening period were 23% and 25% greater than corresponding values of ewes during early fattening period. In conclusion, the effect of age upon energy requirement parameters in the present study were similar in tendency with previous recommendations, values of energy requirement for growth ($NE_g$ and $ME_g$) for Dorper and Hu crossbred female lambs ranged between the NRC (2007) recommendation for early and later maturating growing sheep.