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- 저자 : Guralamatta Siddappa, Ravi Kumara (검색결과 1 건)
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Pandith, Anup,Bhattarai, Kashi Raj,Guralamatta Siddappa, Ravi Kumara,Chae, Han-Jung,Seo, Young Jun Elsevier 2019 Sensors and actuators. B Chemical Vol.282 No.-
<P><B>Abstract</B></P> <P>A doubly armed hydrazone-based <B>FLRHYDDFP</B> probe selectively detects Cu<SUP>2+</SUP> and pyrophosphate (PPi) ions through an colorimetric response-<I>“colorless → yellow → colorless</I>”- as well as “<I>on-off-on</I>” photonic switching response under physiological conditions in a sequential manner. The binding stoichiometries of the analytes Cu<SUP>2+</SUP> and PPi were 1:2 and 2:4 for <B>FLRHYDDFP</B>-Cu<SUP>2+</SUP> and Cu<SUP>2+</SUP>/PPi, respectively. The sequential sensing ability of <B>FLRHYDDFP</B> toward Cu<SUP>2+</SUP> and PPi, attributed to effective complexation-aided d→π* electron transfer (ET) from Cu<SUP>2+</SUP> to <B>FLRHYDDFP</B> and intramolecular charge/electron transfer from <B>FLRHYDDFP</B> to <B>FLRHYDDFP<SUP>+</SUP> </B>, resulted in the formation of a non-symmetric Cu<SUP>2+</SUP> chelate that provided a yellow-colored solution with a significant bathochromic shift from 376 to 446 nm in the UV–vis spectrum and quenching in the emission spectrum. Upon addition of PPi, Cu<SUP>2+</SUP> was extruded from the complex, resulting in a revival of the fluorescence centered at 572 nm. Thus, sequential addition of Cu<SUP>2+</SUP> and PPi yielded a colorless–yellow–colorless transition under visible light and <I>on-off-on</I> switching under 365-nm light (fluorescence). The lowest detection limits for Cu<SUP>2+</SUP> and PPi, when using colorimetric and fluorimetric methods, were in the sub-micromolar and nanomolar levels, respectively. By exploiting this PPi sensing strategy, invitro as well as endogenous alkaline phosphatase activity could be monitored effectively, as demonstrated by exploiting the intracellular production or residual PPi in human salivary glands (normal) and cancer cell lines.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Novel and highly sensitive colorimetric and fluorimetric probe <B>FLRHYDDFP</B> designed and synthesized to identify the Cu<SUP>2+</SUP> and PPi ions in invitro studies with revsersibility. </LI> <LI> By the aid of PPi sensing strategy, alkaline phosphatase activities (ALP) were monitored with lowest detection limit of 0.012 U/mL. </LI> <LI> For the first time endogenous PPi productions aided ALP recognition demonstrated using fluorescence confocal image guided technique in the presence of ALP inhibitor. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
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