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Optimization of extraction process for bioactive compounds from Litsea cubeba fruits
Chih-Lun Cheng,Gui-Bing Hong 한국화학공학회 2018 Korean Journal of Chemical Engineering Vol.35 No.1
Response surface methodology (RSM) was applied to evaluate the extraction of active compounds from Litsea cubeba fruits. A central composite design (CCD) with five levels and three process parameters (extraction temperature, extraction time and ethanol concentration) was used to investigate the influence of the extraction temperature, extraction time and ethanol concentration on the multiple response variables (TPC, DPPH and ABTS assays). The results of the ANOVA analysis show that the quadratic term of the ethanol concentration was significant for all the response variables. The quadratic model was highly significant (P<0.05) for all the response variables. After optimizing for multi-response, the optimal conditions were determined as an extraction temperature of 42.3 oC, extraction time of 126.4min and ethanol concentration of 51%. Moreover, the extract of Litsea cubeba fruit attested possessing tyrosinase inhibitory activity, with an IC50 value of 5,720 μg mL−1. The second-order kinetic model represented the kinetic data very well. In addition, the obtained values of the effective diffusivities were in the range of 2.28-5.83×10−11m2s−1.
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( Jia Cheng ),( Na Sun ),( Xin Zhao ),( Li Niu ),( Mei Qin Song ),( Yao Gui Sun ),( Jun Bing Jiang ),( Jian Hua Guo2 ),( Yuan Sheng Bai ),( Jun Ping He ),( Hong Quan Li ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.8
Seventeen compounds derived from traditional Chinese medicines (TCMs) were tested for their antiviral activity against porcine reproductive and respiratory syndrome virus (PRRSV) in vitro. Visualization with the cytopathologic effect (CPE) assay and the 3-(4, 5-dimethyithiazol- 2-yl)-2,5-diphenyltetrazolium bromide test were used to determine the 50% cytotoxic concentration (CC50) and 50% effective concentration (EC50) in cultured Marc-145 cells. Among the tested compounds, chlorogenic acid and scutellarin showed potential anti-PRRSV activity. The EC50 values were 270.8 ± 14.6 μg/ml and 28.21 ± 26.0 μg/ml and the selectivity indexes were >5.54 and 35.5, respectively. The time-of-addition and virucidal assay indicated that the anti-PRRSV activity of the two compounds could be due to their inhibiting the early stage of virus replication and/or inactivating the virus directly. The inhibition of the virus attachment was not observed in the adsorption inhibition assay. The inhibition ratios of chlorogenic acid and scutellarin were, respectively, 90.8% and 61.1% at the maximum non-cytotoxic concentrations. The results have provided a basis for further exploration of their antiviral properties and mechanisms in vivo. We believe that the chlorogenic acid and scutellarin have a great potential to be developed as new anti-PRRSV drugs for clinical application.
ScienceON
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