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Daniel Wils,Fabrice Desailly,Caroline Perreau,Sophie Grard,Pariyarath Sangeetha Thondre,Lis Ahlstrom,Jonathan Tammam 한국식품영양과학회 2023 Journal of medicinal food Vol.26 No.10
Diabetes prevalence achieved 470B in 2021. Diabetics are looking for foods that allow them to better managethe postprandial glycemia. Owing to its large amylose fraction, pea starch may contribute to formulate recipes with a lowerglycemic index (GI). This study measured the rapidly, slowly digested and resistant fractions in pea starch and in a powdermix recipe. Starch fractions were determined according to the Englyst methodology. A nonblind repeat measure crossoverdesign trial in healthy humans was used to study the GI of pea starch and maltodextrin powder mix recipes against glucose. Gastrointestinal symptoms were measured. Thirteen healthy volunteers aged 18–60 years with body mass index <30 kg/m2 andfasting blood glucose <6.1 mmol/L participated in the study. They consumed 25 g available carbohydrate portions of the testproducts. Blood glucose was measured at -5 and 0 min before consumption till 180 min after starting to eat. The slowdigestible starch (SDS) content of native pea starch was 30% of the total starch content. The pea-based powder mix recipecontained 25% SDS in comparison with 9% for the maltodextrin-based recipe. The glucose response after pea starch wassignificantly lower compared with maltodextrin. The glucose response after pea starch recipe was significantly lower comparedwith maltodextrin recipe. There was no significant difference in mean scores for well-being and gastrointestinalsymptoms after consumption of pea starch and maltodextrin or between the two recipes. In conclusion, this study hasdemonstrated the presence of high SDS content in pea starch, which reduced postprandial glycemic response compared withmaltodextrin. The pea starch recipe did not induce any negative gastrointestinal symptoms. Pea starch may, therefore, prove tobe a beneficial ingredient in developing food products for improving glycemic control without undesirable side effects.
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Use of Ratiometric Probes with a Spectrofluorometer for Bacterial Viability Measurement
( Jerome Cleach ),( Denis Watier ),( Bruno Le Fur ),( Thomas Brauge ),( Guillaume Duflos ),( Thierry Grard ),( Philippe Lencel ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.11
Assessment of microorganism viability is useful in many industrial fields. A large number of methods associated with the use of fluorescent probes have been developed, including fluorimetry, fluorescence microscopy, and cytometry. In this study, a microvolume spectrofluorometer was used to measure the membrane potential variations of Escherichia coli. In order to estimate the sensitivity of the device, the membrane potential of E. coli was artificially disrupted using an ionophore agent: carbonyl cyanide 3-chlorophenylhydrazone. The membrane potential was evaluated using two ratiometric methods: a Rhodamine 123/ 4’,6-diamidino-2-phenylindole combination and a JC-10 ratiometric probe. These methods were used to study the impact of freezing on E. coli, and were compared with the conventional enumeration method. The results showed that it was beneficial to use this compact, easy-touse, and inexpensive spectrofluorometer to assess the viability of bacterial cells via their membrane potential.
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