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RISS 인기검색어
- 검색키워드
- 저자 : Govind C. Sharma (검색결과 2 건)
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Nyaku, Seloame T.,Kantety, Ramesh V.,Cebert, Ernst,Lawrence, Kathy S.,Honger, Joseph O.,Sharma, Govind C. The Korean Society of Plant Pathology 2016 Plant Pathology Journal Vol.32 No.2
U.S. cotton production is suffering from the yield loss caused by the reniform nematode (RN), Rotylenchulus reniformis. Management of this devastating pest is of utmost importance because, no upland cotton cultivar exhibits adequate resistance to RN. Nine populations of RN from distinct regions in Alabama and one population from Mississippi were studied and thirteen morphometric features were measured on 20 male and 20 female nematodes from each population. Highly correlated variables (positive) in female and male RN morphometric parameters were observed for body length (L) and distance of vulva from the lip region (V) (r = 0.7) and tail length (TL) and c' (r = 0.8), respectively. The first and second principal components for the female and male populations showed distinct clustering into three groups. These results show pattern of sub-groups within the RN populations in Alabama. A one-way ANOVA on female and male RN populations showed significant differences ($p{\leq}0.05$) among the variables. Multiple sequence alignment (MSA) of 18S rRNA sequences (421) showed lengths of 653 bp. Sites within the aligned sequences were conserved (53%), parsimony-informative (17%), singletons (28%), and indels (2%), respectively. Neighbor-Joining analysis showed intra and inter-nematodal variations within the populations as clone sequences from different nematodes irrespective of the sex of nematode isolate clustered together. Morphologically, the three groups (I, II and III) could not be distinctly associated with the molecular data from the 18S rRNA sequences. The three groups may be identified as being non-geographically contiguous.
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Seloame T. Nyaku,Ramesh V. Kantety,Ernst Cebert,Kathy S. Lawrence,Joseph O. Honger,Govind C. Sharma 한국식물병리학회 2016 Plant Pathology Journal Vol.32 No.2
U.S. cotton production is suffering from the yield losscaused by the reniform nematode (RN), Rotylenchulusreniformis. Management of this devastating pest is ofutmost importance because, no upland cotton cultivarexhibits adequate resistance to RN. Nine populationsof RN from distinct regions in Alabama and onepopulation from Mississippi were studied and thirteenmorphometric features were measured on 20 male and20 female nematodes from each population. Highly correlatedvariables (positive) in female and male RN morphometricparameters were observed for body length(L) and distance of vulva from the lip region (V) (r = 0.7)and tail length (TL) and c’ (r = 0.8), respectively. Thefirst and second principal components for the femaleand male populations showed distinct clustering intothree groups. These results show pattern of sub-groupswithin the RN populations in Alabama. A one-wayANOVA on female and male RN populations showedsignificant differences (p ≤ 0.05) among the variables.Multiple sequence alignment (MSA) of 18S rRNA sequences(421) showed lengths of 653 bp. Sites within thealigned sequences were conserved (53%), parsimonyinformative(17%), singletons (28%), and indels (2%),respectively. Neighbor-Joining analysis showed intraand inter-nematodal variations within the populationsas clone sequences from different nematodes irrespectiveof the sex of nematode isolate clustered together. Morphologically, the three groups (I, II and III) couldnot be distinctly associated with the molecular datafrom the 18S rRNA sequences. The three groups maybe identified as being non-geographically contiguous.
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