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Realizing the significance of noncoding functionality in clinical genomics
Brian S. Gloss,Marcel E. Dinger 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-
Clinical genomics promises unprecedented precision in understanding the genetic basis of disease. Understanding the impact of variation across the genome is required to realize this potential. Currently, clinical genomics analyses focus on protein-coding genes. However, the noncoding genome is substantially larger than the protein-coding counterpart, and contains structural, regulatory, and transcribed information that needs to be incorporated into genome annotations if the full extent of the opportunity to use genomic information in healthcare is to be realized. This article reviews the challenges and opportunities in unlocking the clinical significance of coding and noncoding genomic information and translating its utility in practice.
Zhao, Shengli,Ting, Jonathan T,Atallah, Hisham E,Qiu, Li,Tan, Jie,Gloss, Bernd,Augustine, George J,Deisseroth, Karl,Luo, Minmin,Graybiel, Ann M,Feng, Guoping Nature Publishing Group, a division of Macmillan P 2011 Nature methods Vol.8 No.9
Optogenetic methods have emerged as powerful tools for dissecting neural circuit connectivity, function and dysfunction. We used a bacterial artificial chromosome (BAC) transgenic strategy to express the H134R variant of channelrhodopsin-2, ChR2(H134R), under the control of cell type??specific promoter elements. We performed an extensive functional characterization of the newly established VGAT-ChR2(H134R)-EYFP, ChAT-ChR2(H134R)-EYFP, Tph2-ChR2(H134R)-EYFP and Pvalb(H134R)-ChR2-EYFP BAC transgenic mouse lines and demonstrate the utility of these lines for precisely controlling action-potential firing of GABAergic, cholinergic, serotonergic and parvalbumin-expressing neuron subsets using blue light. This resource of cell type??specific ChR2(H134R) mouse lines will facilitate the precise mapping of neuronal connectivity and the dissection of the neural basis of behavior.
Next-generation transgenic mice for optogenetic analysis of neural circuits
Asrican, Brent,Augustine, George J.,Berglund, Ken,Chen, Susu,Chow, Nick,Deisseroth, Karl,Feng, Guoping,Gloss, Bernd,Hira, Riichiro,Hoffmann, Carolin,Kasai, Haruo,Katarya, Malvika,Kim, Jinsook,Kudolo, Frontiers Media S.A. 2013 Frontiers in neural circuits Vol.7 No.-
<P>Here we characterize several new lines of transgenic mice useful for optogenetic analysis of brain circuit function. These mice express optogenetic probes, such as enhanced halorhodopsin or several different versions of channelrhodopsins, behind various neuron-specific promoters. These mice permit photoinhibition or photostimulation both <I>in vitro</I> and <I>in vivo</I>. Our results also reveal the important influence of fluorescent tags on optogenetic probe expression and function in transgenic mice.</P>