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Nash, S . R .,Giros, B .,Kingsmore, S . F .,Kim, K . M .,EL-Mestikawy, S .,Dong, Q .,Fumagalli, F .,Seldin, M . F .,Caron, M . G . 전남대학교 약품개발연구소 1998 약품개발연구지 Vol.7 No.1
Two genes were identified and characterized that express cDNAs related to previously identified neurotransmitter and/or osmolyte to transporters. but which are expressed specifically in the kidney. RNA transcribed from one of these two genes (XT2) was found to undergo an extensive degree of alternative splicing to generate six distinct isoforms. The intron-exon structure of the XT2 gene and the site: of alternative, splicing were. identified Expression of the second gene (XT3) was found to be conserved in human kidney. and partial sequence was obtained from a human cDNA library. The expressions of both XT2 and XT3 RNAs were determined in mouse and human tissues, respectively, and the locations of the two genes within the mouse genome were identified. Screening experiments to identity the substrate(s) of these proteins failed to identify specific uptake with any of the tested compounds; however, immunofluorescent microscopy demonstrated that epitope-tagged variants of the protein products of the XT2 and XT3 cDNAs were present on the plasma membrane of transfected rolls.
Biliary Peritonitis after Radiofrequency Ablation Diagnosed by Gadoxetic Acid-Enhanced MR Imaging
Akihiro Furuta,Hiroyoshi Isoda,Takashi Koyama,Giro Todo,Yukio Osaki,Kaori Togashi 대한영상의학회 2013 Korean Journal of Radiology Vol.14 No.6
This study describes the first case of biliary peritonitis after radiofrequency ablation diagnosed by magnetic resonance (MR) imaging with gadolinium ethoxybenzyl diethylenetriaminepentaacetic acid (Gd-EOB-DTPA), a hepatocyte-specific MR imaging contrast agent. The image acquired 300 minutes after the administration of Gd-EOB-DTPA was useful to make a definite diagnosis and to identify the pathway of bile leakage. It is important to decide on the acquisition timing with consideration of the predicted location of bile duct injury.
Kim, Kyeong Man,Kingsmore, Stephen F .,Han, Hong,Yang Feng, Teresa L .,Godinot, Nathalie,Seldin, Michael F .,Caron, Marc G .,Giros, Bruno 전남대학교 약품개발연구소 1995 약품개발연구지 Vol.3 No.1
We report the molecular cloning of a cDNA encoding a high affinity human glycine transporter. An open reading frame of 1914 nucleotides encodes a 638-amino acid protein that transports glycine in a Na^+/Cl^--dependent manner. In common with other Na^+/Cl^-dependent transporters, it possesses 12 putative transmembrane domains, according to its hydropathicity profile. This protein is the human homologue of a glycine transporter previously isolated from rat [glycine transporter type 1b (GlyT-1b)]. In addition to the human GlyT-1b, we also characterized a novel functional isoform produced by alternative splicing. This isoform, GlyT-1c, which is distinct from GlyT-2 recently characterized in rat, contains an additional exon encoding 54 amino acids in the amino-terminal part of GlyT-1 b and is mainly expressed in brain. These two isoforms are products of the same gene and are localized on human chromosome 1 p31.3, as well as on mouse chromosome 4, close to the locus for the spontaneous mouse neuromuscular mutation clasper. When expressed in COS-7 cells, both the human GlyT-1b and GlyT-1c display a time- and dose-dependent uptake of glycine, which is abolished when either Na^+ or Cl^- is substituted with other ions. For both GlyT-1b and GlyT-1c the affinities for glycine are similar, with K_m values of 70-90 μm, and this uptake is inhibited by sarcosine with similar potencies. In addition to the three transporter isoforms present in the human genome, i.e., GlyT-1a, GlyT-1b, and GlyT-1c, point-mutated variants, which appear to be totally devoid of glycine uptake activity when expressed in COS-7 cells, were obtained by polymerase chain reaction amplification of mRNA from human substantia nigra. These variants point to regions of the glycine transporter that might be important in the processing or transport function of this protein.
R. M. Esparza-Merino,M. E. Macı´as-Rodrı´guez,E. Cabrera-Dı´az,A. J. Valencia-Botı´n,Y. Estrada-Giro´n 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.4
The by-products of Hibiscus sabdariffa L. (HsL), obtained after soaking or decoction of the calyces ofColima and Sudan cultivars, were used for pectin extraction. After soaking, the wastes of both cultivars gave higheryields of pectin than those obtained by decoction. Thepectin of the wastes by soaking presented high methoxylgroups, galacturonic acid content, viscosity and gellingcapacity. Pectin of this treatment also exhibited bands inthe regions of 1750 cm-1 and 1630 cm-1 that representsthe C=O stretching vibrations of methyl ester and theamounts and degree of esterification of the HsL pectin. Interestingly, the pectin retained the typical red color offresh HsL calyces. The amounts of anthocyanins andascorbic acid of pectin did not show effects againstpathogenic microorganisms. Nonetheless, pectin of theSudan HsL wastes obtained by soaking, exhibited higherproperties than those of the citric pectin, thus, demonstratingits potential for industrial applications.