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Ghim, Sa-Youl Korean Society of Life Science 2001 Journal of Life Science Vol.11 No.2
The pyrR gene of the pyrimidine biosynthesis (pyr) operon of the thermophile Bacillus caldolyticus, encoding a uracil phosphoribosyltransferase (UPRTase), turned to rely as a pyr operon regulator. It has been proposed that PyrR mediates transcriptional termination-antitermination at three intercistronic regions of the par operon (S.-Y Ghim and J. Neuhard, J. Bacteriol.,176, 3698-3707, 1994). In this research, a plasmid carrying the pyrR region of B. caldolyticus could restore a pyrimidine regulation in a pyrR mutant of B. subtilis. Expression of pyrR was found to increase 6-7 fold during pyrimidine starvation. Additionally, a highly conserved nucleotide sequence which may constitute the binding site for a PyrR protein (PyrR-binding loop) in transcript was staggested. Alternative antiterminator and terminator structures involving three conserved motifs in front of the pyrR, pyrP and pyrB genes, respectively, are proposed to account for the observed regulation pattern.
Spectrally resolved white-light interferometry for 3D inspection of a thin-film layer structure
Ghim, Young-Sik,Kim, Seung-Woo The Optical Society 2009 Applied Optics Vol.48 No.4
<P>We describe an improved scheme of spectrally resolved white-light interferometry, which provides 3D visual inspection of a thin-film layer structure with nanometer level resolutions. Compared to the authors' previous method [Appl. Phys. Lett.91, 091903 (2007)APPLAB0003-695110.1063/1.2776015], 3D tomographic information of thin films can be obtained by decoupling the film thickness and top surface profile, which is embodied by inducing spectral carrier frequency to the reference arm and applying a low-pass filter to the interferogram instead of two troublesome measurement steps of activating and deactivating a mechanical shutter. We test and verify our proposed method by measuring a patterned thin-film layer structure as well as standard specimens of thin films with various thicknesses.</P>
Synthetic Biology for Biofuels: Building Designer Microbes from the Scratch
Ghim, Cheol-Min,Kim, Tae-Sung,Mitchell, Robert J.,Lee, Sung-Kuk 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.1
The ultimate goal in the production of biofuels is to produce fuels identical or similar to petroleum-derived transportation fuels more efficiently and in commercial quantities. Synthetic biologists have been engineering microbes to synthesize biofuels, such as butanol and fatty acid- or isoprenoid-based fuels, which are nearly identical to gasoline and diesel. One of the most urgent demands along this direction is to attain a solid framework for characterizing and standardizing the biological parts and devices. It seems quite promising because biotechnologies specially based on miniaturizations have been making a big contribution to this work. Therefore, in this review, recent advances and difficulties in the biofuel field are discussed, along with the advances of synthetic biology, which will make it possible to create designer microorganisms that produce economically viable next generation biofuels, aside from bioethanol, from corn or sugar cane, and biodiesel from plant or animal oils.
Condensation heat transfer of low GWP ORC working fluids in a horizontal smooth tube
Ghim, G.,Lee, J. Pergamon Press ; Elsevier Science Ltd 2017 INTERNATIONAL JOURNAL OF HEAT AND MASS TRANSFER - Vol.104 No.-
The objective of the current study is to conduct an experimental investigation of condensation heat transfer for the ORC (organic Rankine cycle) working fluids R245fa, Novec®649 and HFE-7000 over a wide range of parameters. Quasi-local condensation heat transfer coefficients are measured in a straight horizontal 7.75-mm-ID smooth tube with flow conditions at mass flux G=150-700kg (m<SUP>2</SUP>-s)<SUP>-1</SUP>, local equilibrium quality x=0.05-0.95, and reduced pressure, P<SUB>r</SUB>=0.05-0.15. Test data reveal that the environmentally friendly fluids have lower condensation heat transfer coefficients when compared to R245fa. The 141 measured condensation heat transfer coefficients are compared to six well-known correlations. Among the six correlations verified with test data, the Shah correlation shows the best predictive performance for the three fluids tested.
RAPID PURIFICATION AND MOLECULAR CHARACTERIZATION OF HUMAN NEUTROPHIL ELASTASE
Ghim, Sa-Youl,Bae, Sung Jun,Kang, Kooil 고신대학교 의학부 1989 高神大學校 醫學部 論文集 Vol.5 No.1
Elastase에 의해 야기되는 질병의 예방 및 치료 응용을 위하여는, 먼저 이 효소가 고순도로 분리되고 그 근본적인 성질의 해명이 이루어져야 한다. 고순도의 elastase를 얻기위해, 사람의 전혈로부터 Hypaque-Ficoll step gradient에 의해 순수한 neutrophil을 얻은 다음 이것을 extract로 만들어 액체크로마토그래피를 실시하였다. Ultrogel AcA54 gel filtration을 거친 후 CM-sephadex 이온교환 크로마토그래피를 적용하였는데, 이때 유출 완충액 속에 0.1% Brij 35를 첨가하여 elastase의 순도를 더욱 높일 수 있었다. 이 정제된 elastase는 SDS-PAGE를 통해 MW 28,000, 29,000, 30,000의 서로 다른 3개 의 밴드로 확인되었으며, 그간 수많은 연구자들간에 elastase의 분자량에 대해 심한 견해 차이를 보여온 것은 그 분자적 성질이나 분자량이 유사한 cathepsin G가 혼재하거나 elastase분자내 혹은 분자간 S-S결합의 차이에 의함을 알 수 있었다.
Ghim, G.,Lee, J. Pergamon ; Elsevier Science Ltd 2016 Applied thermal engineering Vol.106 No.-
The existence of temperature gliding in non-azeotropic mixture working fluids provides a promising opportunity for greatly improved resource utilization in organic Rankine cycle (ORC) systems for geothermal applications. The condensation heat transfer degradation associated with working fluid mixtures is an unavoidable penalty that may reduce the utilization and system benefits by necessitating larger heat exchangers. The objective of the current study is an experimental investigation of condensation heat transfer for R245fa, n-pentane, and a composition of their mixture. Quasi-local condensation heat transfer coefficients are measured in a straight horizontal smooth tube with flow conditions at mass flux G=100 and 150kg(m<SUP>2</SUP>s)<SUP>-1</SUP>, local equilibrium quality x=0.05-0.80, and reduced pressures P<SUB>r</SUB>=0.05-0.15. The reduced condensation heat transfer coefficients from the test are compared with the predictions from already established mixture correction methods in the literature. Pure components R245fa and n-pentane condensation heat transfer data are well predicted by Shah (1979) correlations for all test conditions. However, their non-azeotropic mixture condensation heat transfer data require additional correction parameter to account for the heat transfer degradation. This correction parameter is correlated by the temperature gliding gradient of mixture fluids. Test data with mixture working fluids from current study are reasonably well predicted by the corrected in-tube correlation.